Experiment: PYE with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00030 and CCNA_00031 overlap by 18 nucleotides CCNA_00031 and CCNA_00032 are separated by 0 nucleotides CCNA_00032 and CCNA_03912 are separated by 105 nucleotides CCNA_03912 and CCNA_00033 are separated by 156 nucleotides
CCNA_00030: CCNA_00030 - ribonuclease T2, at 33,595 to 34,329
_00030
CCNA_00031: CCNA_00031 - toxin protein VapC, at 34,312 to 34,698
_00031
CCNA_00032: CCNA_00032 - antitoxin protein VapB, at 34,699 to 34,941
_00032
CCNA_03912: CCNA_03912 - hypothetical protein, at 35,047 to 35,667
_03912
CCNA_00033: CCNA_00033 - polyribonucleotide nucleotidyltransferase/polynucleotide adenylyltransferase, at 35,824 to 37,962
_00033
Position (kb)
34
35 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 33.704 kb on - strand, within CCNA_00030 at 33.749 kb on + strand, within CCNA_00030 at 33.757 kb on - strand, within CCNA_00030 at 33.757 kb on - strand, within CCNA_00030 at 33.777 kb on - strand, within CCNA_00030 at 33.781 kb on + strand, within CCNA_00030 at 33.789 kb on - strand, within CCNA_00030 at 33.789 kb on - strand, within CCNA_00030 at 33.794 kb on - strand, within CCNA_00030 at 33.796 kb on + strand, within CCNA_00030 at 33.796 kb on + strand, within CCNA_00030 at 33.804 kb on - strand, within CCNA_00030 at 33.825 kb on - strand, within CCNA_00030 at 33.856 kb on + strand, within CCNA_00030 at 33.871 kb on + strand, within CCNA_00030 at 33.871 kb on + strand, within CCNA_00030 at 33.871 kb on - strand, within CCNA_00030 at 33.879 kb on - strand, within CCNA_00030 at 33.879 kb on - strand, within CCNA_00030 at 33.879 kb on - strand, within CCNA_00030 at 33.917 kb on + strand, within CCNA_00030 at 33.942 kb on + strand, within CCNA_00030 at 33.950 kb on - strand, within CCNA_00030 at 33.974 kb on - strand, within CCNA_00030 at 33.974 kb on - strand, within CCNA_00030 at 33.999 kb on + strand, within CCNA_00030 at 34.007 kb on - strand, within CCNA_00030 at 34.012 kb on + strand, within CCNA_00030 at 34.042 kb on - strand, within CCNA_00030 at 34.068 kb on - strand, within CCNA_00030 at 34.135 kb on + strand, within CCNA_00030 at 34.135 kb on + strand, within CCNA_00030 at 34.155 kb on + strand, within CCNA_00030 at 34.172 kb on + strand, within CCNA_00030 at 34.176 kb on + strand, within CCNA_00030 at 34.177 kb on + strand, within CCNA_00030 at 34.180 kb on - strand, within CCNA_00030 at 34.180 kb on - strand, within CCNA_00030 at 34.184 kb on - strand, within CCNA_00030 at 34.191 kb on + strand, within CCNA_00030 at 34.199 kb on - strand, within CCNA_00030 at 34.203 kb on - strand, within CCNA_00030 at 34.214 kb on + strand, within CCNA_00030 at 34.224 kb on - strand, within CCNA_00030 at 34.226 kb on + strand, within CCNA_00030 at 34.234 kb on - strand, within CCNA_00030 at 34.239 kb on - strand, within CCNA_00030 at 34.278 kb on - strand at 34.316 kb on - strand at 34.387 kb on + strand, within CCNA_00031 at 34.388 kb on + strand, within CCNA_00031 at 34.403 kb on - strand, within CCNA_00031 at 34.414 kb on + strand, within CCNA_00031 at 34.434 kb on - strand, within CCNA_00031 at 34.455 kb on - strand, within CCNA_00031 at 34.526 kb on - strand, within CCNA_00031 at 34.526 kb on - strand at 34.544 kb on + strand, within CCNA_00031 at 34.552 kb on - strand, within CCNA_00031 at 34.568 kb on + strand, within CCNA_00031 at 34.581 kb on - strand, within CCNA_00031 at 34.600 kb on - strand, within CCNA_00031 at 34.615 kb on + strand, within CCNA_00031 at 34.615 kb on + strand, within CCNA_00031 at 34.615 kb on + strand, within CCNA_00031 at 34.615 kb on + strand, within CCNA_00031 at 34.622 kb on + strand, within CCNA_00031 at 34.623 kb on - strand, within CCNA_00031 at 34.623 kb on - strand, within CCNA_00031 at 34.623 kb on - strand, within CCNA_00031 at 34.623 kb on - strand, within CCNA_00031 at 34.623 kb on - strand, within CCNA_00031 at 34.623 kb on - strand, within CCNA_00031 at 34.623 kb on - strand, within CCNA_00031 at 34.623 kb on - strand, within CCNA_00031 at 34.623 kb on - strand, within CCNA_00031 at 34.623 kb on - strand, within CCNA_00031 at 34.623 kb on - strand, within CCNA_00031 at 34.630 kb on - strand, within CCNA_00031 at 34.630 kb on - strand, within CCNA_00031 at 34.630 kb on - strand, within CCNA_00031 at 34.705 kb on - strand at 34.819 kb on - strand, within CCNA_00032 at 34.824 kb on - strand, within CCNA_00032 at 34.824 kb on - strand, within CCNA_00032 at 34.824 kb on - strand, within CCNA_00032 at 34.824 kb on - strand, within CCNA_00032 at 34.856 kb on - strand, within CCNA_00032 at 34.867 kb on + strand, within CCNA_00032 at 34.992 kb on - strand at 35.019 kb on + strand at 35.026 kb on + strand at 35.055 kb on + strand at 35.055 kb on + strand at 35.151 kb on + strand, within CCNA_03912 at 35.174 kb on - strand, within CCNA_03912 at 35.189 kb on - strand, within CCNA_03912 at 35.265 kb on - strand, within CCNA_03912 at 35.279 kb on + strand, within CCNA_03912 at 35.282 kb on - strand, within CCNA_03912 at 35.300 kb on + strand, within CCNA_03912 at 35.353 kb on + strand, within CCNA_03912 at 35.361 kb on - strand, within CCNA_03912 at 35.361 kb on - strand, within CCNA_03912 at 35.397 kb on - strand, within CCNA_03912 at 35.425 kb on + strand, within CCNA_03912 at 35.425 kb on + strand, within CCNA_03912 at 35.487 kb on + strand, within CCNA_03912 at 35.491 kb on - strand, within CCNA_03912 at 35.501 kb on + strand, within CCNA_03912 at 35.514 kb on - strand, within CCNA_03912 at 35.535 kb on + strand, within CCNA_03912 at 35.558 kb on + strand, within CCNA_03912 at 35.558 kb on + strand, within CCNA_03912 at 35.585 kb on + strand, within CCNA_03912 at 35.592 kb on + strand, within CCNA_03912 at 35.593 kb on + strand, within CCNA_03912 at 35.604 kb on + strand, within CCNA_03912 at 35.612 kb on - strand at 35.612 kb on - strand at 35.623 kb on - strand at 35.639 kb on + strand at 35.658 kb on - strand at 35.661 kb on + strand at 35.669 kb on - strand at 35.741 kb on - strand at 35.822 kb on + strand at 35.830 kb on - strand at 35.830 kb on - strand at 35.854 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Dimethyl Sulfoxide 7.5 vol% remove 33,704 - CCNA_00030 0.15 -0.3 33,749 + CCNA_00030 0.21 +1.6 33,757 - CCNA_00030 0.22 -0.0 33,757 - CCNA_00030 0.22 -0.0 33,777 - CCNA_00030 0.25 -1.8 33,781 + CCNA_00030 0.25 -0.5 33,789 - CCNA_00030 0.26 -0.8 33,789 - CCNA_00030 0.26 -0.2 33,794 - CCNA_00030 0.27 -0.3 33,796 + CCNA_00030 0.27 +0.8 33,796 + CCNA_00030 0.27 +0.7 33,804 - CCNA_00030 0.28 +2.0 33,825 - CCNA_00030 0.31 -1.1 33,856 + CCNA_00030 0.36 -2.1 33,871 + CCNA_00030 0.38 +0.2 33,871 + CCNA_00030 0.38 -1.5 33,871 - CCNA_00030 0.38 -0.0 33,879 - CCNA_00030 0.39 +2.0 33,879 - CCNA_00030 0.39 -2.3 33,879 - CCNA_00030 0.39 +1.2 33,917 + CCNA_00030 0.44 -0.3 33,942 + CCNA_00030 0.47 -0.6 33,950 - CCNA_00030 0.48 +0.1 33,974 - CCNA_00030 0.52 -0.7 33,974 - CCNA_00030 0.52 -0.3 33,999 + CCNA_00030 0.55 -1.2 34,007 - CCNA_00030 0.56 +0.4 34,012 + CCNA_00030 0.57 +0.6 34,042 - CCNA_00030 0.61 -1.2 34,068 - CCNA_00030 0.64 +0.1 34,135 + CCNA_00030 0.73 -1.6 34,135 + CCNA_00030 0.73 +0.7 34,155 + CCNA_00030 0.76 -0.3 34,172 + CCNA_00030 0.79 +0.7 34,176 + CCNA_00030 0.79 +0.2 34,177 + CCNA_00030 0.79 -2.3 34,180 - CCNA_00030 0.80 -0.4 34,180 - CCNA_00030 0.80 -0.6 34,184 - CCNA_00030 0.80 -0.4 34,191 + CCNA_00030 0.81 -0.3 34,199 - CCNA_00030 0.82 +0.1 34,203 - CCNA_00030 0.83 -1.0 34,214 + CCNA_00030 0.84 +2.1 34,224 - CCNA_00030 0.86 -0.5 34,226 + CCNA_00030 0.86 +2.7 34,234 - CCNA_00030 0.87 +0.3 34,239 - CCNA_00030 0.88 +1.7 34,278 - -2.0 34,316 - -1.1 34,387 + CCNA_00031 0.19 +0.4 34,388 + CCNA_00031 0.20 -1.2 34,403 - CCNA_00031 0.24 +1.7 34,414 + CCNA_00031 0.26 +1.0 34,434 - CCNA_00031 0.32 +2.4 34,455 - CCNA_00031 0.37 +2.2 34,526 - CCNA_00031 0.55 +2.9 34,526 - +3.6 34,544 + CCNA_00031 0.60 -2.5 34,552 - CCNA_00031 0.62 +2.3 34,568 + CCNA_00031 0.66 +0.3 34,581 - CCNA_00031 0.70 +2.5 34,600 - CCNA_00031 0.74 +2.4 34,615 + CCNA_00031 0.78 -1.2 34,615 + CCNA_00031 0.78 -0.0 34,615 + CCNA_00031 0.78 -0.3 34,615 + CCNA_00031 0.78 -1.6 34,622 + CCNA_00031 0.80 -2.3 34,623 - CCNA_00031 0.80 +2.5 34,623 - CCNA_00031 0.80 +1.8 34,623 - CCNA_00031 0.80 +1.9 34,623 - CCNA_00031 0.80 +2.4 34,623 - CCNA_00031 0.80 +2.5 34,623 - CCNA_00031 0.80 +2.2 34,623 - CCNA_00031 0.80 +2.8 34,623 - CCNA_00031 0.80 +2.8 34,623 - CCNA_00031 0.80 +2.2 34,623 - CCNA_00031 0.80 +2.5 34,623 - CCNA_00031 0.80 +2.7 34,630 - CCNA_00031 0.82 +3.0 34,630 - CCNA_00031 0.82 +2.6 34,630 - CCNA_00031 0.82 +2.2 34,705 - +0.3 34,819 - CCNA_00032 0.49 +2.6 34,824 - CCNA_00032 0.51 +2.0 34,824 - CCNA_00032 0.51 +2.2 34,824 - CCNA_00032 0.51 +2.5 34,824 - CCNA_00032 0.51 +3.1 34,856 - CCNA_00032 0.65 +2.6 34,867 + CCNA_00032 0.69 -0.0 34,992 - -0.8 35,019 + -0.8 35,026 + -2.8 35,055 + -0.1 35,055 + +0.3 35,151 + CCNA_03912 0.17 -0.1 35,174 - CCNA_03912 0.20 +0.5 35,189 - CCNA_03912 0.23 +0.5 35,265 - CCNA_03912 0.35 +0.8 35,279 + CCNA_03912 0.37 +0.4 35,282 - CCNA_03912 0.38 +1.0 35,300 + CCNA_03912 0.41 +0.2 35,353 + CCNA_03912 0.49 -0.0 35,361 - CCNA_03912 0.51 -1.3 35,361 - CCNA_03912 0.51 +0.3 35,397 - CCNA_03912 0.56 -0.3 35,425 + CCNA_03912 0.61 -0.6 35,425 + CCNA_03912 0.61 -1.6 35,487 + CCNA_03912 0.71 -2.0 35,491 - CCNA_03912 0.71 -0.0 35,501 + CCNA_03912 0.73 -1.0 35,514 - CCNA_03912 0.75 -0.0 35,535 + CCNA_03912 0.79 -1.0 35,558 + CCNA_03912 0.82 +2.0 35,558 + CCNA_03912 0.82 +0.7 35,585 + CCNA_03912 0.87 +1.0 35,592 + CCNA_03912 0.88 +0.6 35,593 + CCNA_03912 0.88 +0.1 35,604 + CCNA_03912 0.90 +0.4 35,612 - +0.4 35,612 - +1.5 35,623 - +1.2 35,639 + -1.0 35,658 - -0.2 35,661 + +0.2 35,669 - +0.6 35,741 - +0.1 35,822 + -1.1 35,830 - +2.4 35,830 - +2.8 35,854 + -0.1
Or see this region's nucleotide sequence