Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25570 and AO356_25575 are separated by 52 nucleotides AO356_25575 and AO356_25580 are separated by 84 nucleotides AO356_25580 and AO356_25585 are separated by 215 nucleotides
AO356_25570: AO356_25570 - MFS transporter, at 5,780,018 to 5,781,322
_25570
AO356_25575: AO356_25575 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 5,781,375 to 5,781,767
_25575
AO356_25580: AO356_25580 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 5,781,852 to 5,782,709
_25580
AO356_25585: AO356_25585 - 2-hydroxymuconic semialdehyde dehydrogenase, at 5,782,925 to 5,784,385
_25585
Position (kb) 5781
5782
5783 Strain fitness (log2 ratio) -1
0
1
2 at 5781.592 kb on + strand, within AO356_25575 at 5781.746 kb on - strand at 5781.804 kb on + strand at 5781.805 kb on - strand at 5782.313 kb on - strand, within AO356_25580 at 5782.313 kb on - strand, within AO356_25580 at 5782.442 kb on - strand, within AO356_25580 at 5782.519 kb on + strand, within AO356_25580 at 5782.520 kb on - strand, within AO356_25580 at 5782.661 kb on - strand at 5782.884 kb on + strand at 5782.884 kb on + strand at 5782.896 kb on + strand at 5782.896 kb on + strand at 5782.896 kb on + strand at 5782.943 kb on + strand at 5782.944 kb on - strand at 5783.004 kb on + strand at 5783.005 kb on - strand at 5783.005 kb on - strand at 5783.155 kb on - strand, within AO356_25585 at 5783.670 kb on + strand, within AO356_25585
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 5,781,592 + AO356_25575 0.55 -0.6 5,781,746 - -0.4 5,781,804 + -0.7 5,781,805 - +0.3 5,782,313 - AO356_25580 0.54 +0.4 5,782,313 - AO356_25580 0.54 -0.1 5,782,442 - AO356_25580 0.69 +1.8 5,782,519 + AO356_25580 0.78 +1.7 5,782,520 - AO356_25580 0.78 -0.4 5,782,661 - +0.8 5,782,884 + -0.9 5,782,884 + -0.6 5,782,896 + +0.4 5,782,896 + +1.1 5,782,896 + -0.7 5,782,943 + -0.7 5,782,944 - +0.2 5,783,004 + -0.7 5,783,005 - +0.0 5,783,005 - -1.1 5,783,155 - AO356_25585 0.16 -1.6 5,783,670 + AO356_25585 0.51 +0.5
Or see this region's nucleotide sequence