Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_20830 and AO356_20835 are separated by 174 nucleotides AO356_20835 and AO356_20840 are separated by 147 nucleotides AO356_20840 and AO356_20845 are separated by 281 nucleotides
AO356_20830: AO356_20830 - phosphoadenosine phosphosulfate reductase, at 4,640,680 to 4,641,423
_20830
AO356_20835: AO356_20835 - phosphoserine phosphatase, at 4,641,598 to 4,642,215
_20835
AO356_20840: AO356_20840 - aminodeoxychorismate synthase component I, at 4,642,363 to 4,643,706
_20840
AO356_20845: AO356_20845 - alpha-L-glutamate ligase, at 4,643,988 to 4,644,974
_20845
Position (kb)
4642
4643
4644 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4641.383 kb on + strand at 4641.383 kb on + strand at 4641.462 kb on + strand at 4641.462 kb on + strand at 4641.462 kb on + strand at 4641.462 kb on + strand at 4641.462 kb on + strand at 4641.462 kb on + strand at 4641.462 kb on + strand at 4641.462 kb on + strand at 4641.463 kb on - strand at 4641.463 kb on - strand at 4641.463 kb on - strand at 4641.463 kb on - strand at 4641.878 kb on - strand, within AO356_20835 at 4642.027 kb on + strand, within AO356_20835 at 4642.027 kb on + strand, within AO356_20835 at 4642.028 kb on - strand, within AO356_20835 at 4642.028 kb on - strand, within AO356_20835 at 4642.028 kb on - strand, within AO356_20835 at 4642.031 kb on - strand, within AO356_20835 at 4642.057 kb on + strand, within AO356_20835 at 4642.057 kb on + strand, within AO356_20835 at 4642.057 kb on + strand, within AO356_20835 at 4642.058 kb on - strand, within AO356_20835 at 4642.213 kb on + strand at 4642.214 kb on - strand at 4642.214 kb on - strand at 4642.214 kb on - strand at 4642.214 kb on - strand at 4642.214 kb on - strand at 4642.321 kb on + strand at 4642.321 kb on + strand at 4642.322 kb on - strand at 4642.324 kb on - strand at 4642.364 kb on + strand at 4642.364 kb on + strand at 4642.542 kb on - strand, within AO356_20840 at 4642.542 kb on - strand, within AO356_20840 at 4642.542 kb on - strand, within AO356_20840 at 4643.123 kb on + strand, within AO356_20840 at 4643.123 kb on + strand, within AO356_20840 at 4643.124 kb on - strand, within AO356_20840 at 4643.124 kb on - strand, within AO356_20840 at 4643.134 kb on - strand, within AO356_20840 at 4643.330 kb on + strand, within AO356_20840 at 4643.330 kb on + strand, within AO356_20840 at 4643.411 kb on + strand, within AO356_20840 at 4643.420 kb on + strand, within AO356_20840 at 4643.689 kb on + strand at 4643.690 kb on - strand at 4643.690 kb on - strand at 4643.746 kb on + strand at 4643.746 kb on + strand at 4643.746 kb on + strand at 4643.746 kb on + strand at 4643.756 kb on + strand at 4643.794 kb on + strand at 4643.794 kb on + strand at 4643.795 kb on - strand at 4643.795 kb on - strand at 4644.008 kb on + strand at 4644.008 kb on + strand at 4644.008 kb on + strand at 4644.009 kb on - strand at 4644.009 kb on - strand at 4644.009 kb on - strand at 4644.009 kb on - strand at 4644.220 kb on + strand, within AO356_20845 at 4644.220 kb on + strand, within AO356_20845 at 4644.221 kb on - strand, within AO356_20845 at 4644.241 kb on + strand, within AO356_20845 at 4644.241 kb on + strand, within AO356_20845 at 4644.242 kb on - strand, within AO356_20845 at 4644.242 kb on - strand, within AO356_20845 at 4644.242 kb on - strand, within AO356_20845 at 4644.242 kb on - strand, within AO356_20845 at 4644.505 kb on + strand, within AO356_20845 at 4644.505 kb on + strand, within AO356_20845 at 4644.506 kb on - strand, within AO356_20845 at 4644.583 kb on + strand, within AO356_20845
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,641,383 + -0.6 4,641,383 + -0.3 4,641,462 + -0.0 4,641,462 + +0.1 4,641,462 + +1.0 4,641,462 + +0.3 4,641,462 + +0.3 4,641,462 + -1.4 4,641,462 + +0.4 4,641,462 + -0.1 4,641,463 - -0.3 4,641,463 - +0.8 4,641,463 - +0.3 4,641,463 - -0.3 4,641,878 - AO356_20835 0.45 -1.2 4,642,027 + AO356_20835 0.69 -0.7 4,642,027 + AO356_20835 0.69 +0.9 4,642,028 - AO356_20835 0.70 +1.1 4,642,028 - AO356_20835 0.70 -0.8 4,642,028 - AO356_20835 0.70 -0.5 4,642,031 - AO356_20835 0.70 +0.3 4,642,057 + AO356_20835 0.74 +0.3 4,642,057 + AO356_20835 0.74 -0.2 4,642,057 + AO356_20835 0.74 -0.6 4,642,058 - AO356_20835 0.74 -0.2 4,642,213 + +0.1 4,642,214 - -0.4 4,642,214 - -0.6 4,642,214 - -0.9 4,642,214 - +0.4 4,642,214 - +0.0 4,642,321 + -1.3 4,642,321 + -0.9 4,642,322 - +0.0 4,642,324 - +0.5 4,642,364 + +0.8 4,642,364 + -0.1 4,642,542 - AO356_20840 0.13 -0.9 4,642,542 - AO356_20840 0.13 +0.3 4,642,542 - AO356_20840 0.13 -0.3 4,643,123 + AO356_20840 0.57 -1.2 4,643,123 + AO356_20840 0.57 -0.8 4,643,124 - AO356_20840 0.57 +1.5 4,643,124 - AO356_20840 0.57 -0.4 4,643,134 - AO356_20840 0.57 +0.3 4,643,330 + AO356_20840 0.72 +0.0 4,643,330 + AO356_20840 0.72 -0.5 4,643,411 + AO356_20840 0.78 -0.7 4,643,420 + AO356_20840 0.79 +0.1 4,643,689 + -0.5 4,643,690 - +1.8 4,643,690 - +0.9 4,643,746 + -0.7 4,643,746 + -0.8 4,643,746 + -1.6 4,643,746 + +0.1 4,643,756 + +0.4 4,643,794 + -2.2 4,643,794 + -1.9 4,643,795 - +0.3 4,643,795 - +0.5 4,644,008 + -0.2 4,644,008 + +0.3 4,644,008 + -0.0 4,644,009 - -0.2 4,644,009 - -0.5 4,644,009 - -0.6 4,644,009 - -2.1 4,644,220 + AO356_20845 0.24 -1.6 4,644,220 + AO356_20845 0.24 -0.5 4,644,221 - AO356_20845 0.24 -0.5 4,644,241 + AO356_20845 0.26 -2.6 4,644,241 + AO356_20845 0.26 -0.0 4,644,242 - AO356_20845 0.26 -2.3 4,644,242 - AO356_20845 0.26 -1.1 4,644,242 - AO356_20845 0.26 -1.1 4,644,242 - AO356_20845 0.26 -1.1 4,644,505 + AO356_20845 0.52 -1.7 4,644,505 + AO356_20845 0.52 -2.0 4,644,506 - AO356_20845 0.52 -1.5 4,644,583 + AO356_20845 0.60 -1.1
Or see this region's nucleotide sequence