Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livH and DVU0549 are separated by 27 nucleotides DVU0549 and livG overlap by 1 nucleotides livG and livF overlap by 8 nucleotides
DVU0548: livH - high-affinity branched-chain amino acid ABC transporter, permease protein (TIGR), at 618,218 to 619,126
livH
DVU0549: DVU0549 - proline/arginine ABC transporter, permease component <small>(from data)</small>, at 619,154 to 620,377
DVU0549
DVU0550: livG - high-affinity branched-chain amino acid ABC transporter, ATP binding protein (TIGR), at 620,377 to 621,165
livG
DVU0551: livF - high-affinity branched-chain amino acid ABC transporter ATP-binding protein (TIGR), at 621,158 to 621,862
livF
Position (kb) 619
620
621 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 618.292 kb on + strand at 618.292 kb on + strand at 618.297 kb on + strand at 618.333 kb on - strand, within livH at 618.449 kb on + strand, within livH at 618.464 kb on + strand, within livH at 618.464 kb on + strand, within livH at 618.501 kb on - strand, within livH at 618.521 kb on - strand, within livH at 618.588 kb on - strand, within livH at 618.699 kb on + strand, within livH at 618.706 kb on + strand, within livH at 618.716 kb on - strand, within livH at 618.722 kb on + strand, within livH at 618.739 kb on + strand, within livH at 618.744 kb on + strand, within livH at 618.747 kb on - strand, within livH at 618.747 kb on - strand, within livH at 618.747 kb on - strand, within livH at 618.777 kb on + strand, within livH at 618.801 kb on + strand, within livH at 618.815 kb on - strand, within livH at 618.924 kb on - strand, within livH at 619.060 kb on + strand at 619.113 kb on - strand at 619.245 kb on + strand at 619.361 kb on - strand, within DVU0549 at 619.457 kb on + strand, within DVU0549 at 619.471 kb on + strand, within DVU0549 at 619.518 kb on - strand, within DVU0549 at 619.518 kb on - strand, within DVU0549 at 619.615 kb on - strand, within DVU0549 at 619.616 kb on - strand, within DVU0549 at 619.660 kb on - strand, within DVU0549 at 619.660 kb on - strand, within DVU0549 at 619.678 kb on - strand, within DVU0549 at 619.717 kb on + strand, within DVU0549 at 619.794 kb on + strand, within DVU0549 at 619.877 kb on + strand, within DVU0549 at 619.965 kb on + strand, within DVU0549 at 620.000 kb on + strand, within DVU0549 at 620.007 kb on + strand, within DVU0549 at 620.091 kb on + strand, within DVU0549 at 620.095 kb on - strand, within DVU0549 at 620.100 kb on - strand, within DVU0549 at 620.184 kb on + strand, within DVU0549 at 620.192 kb on - strand, within DVU0549 at 620.322 kb on + strand at 620.455 kb on - strand at 620.459 kb on + strand, within livG at 620.481 kb on + strand, within livG at 620.667 kb on + strand, within livG at 620.797 kb on + strand, within livG at 620.826 kb on + strand, within livG at 620.850 kb on + strand, within livG at 620.850 kb on + strand, within livG at 620.850 kb on + strand, within livG at 620.858 kb on - strand, within livG at 620.858 kb on - strand, within livG at 620.858 kb on - strand, within livG at 620.916 kb on - strand, within livG at 621.000 kb on + strand, within livG at 621.044 kb on - strand, within livG at 621.098 kb on + strand at 621.101 kb on - strand at 621.130 kb on + strand at 621.167 kb on - strand at 621.167 kb on - strand at 621.177 kb on - strand at 621.177 kb on - strand at 621.221 kb on - strand at 621.280 kb on - strand, within livF at 621.280 kb on - strand, within livF at 621.287 kb on - strand, within livF at 621.311 kb on + strand, within livF at 621.321 kb on + strand, within livF
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 618,292 + +1.9 618,292 + +1.4 618,297 + +1.5 618,333 - livH DVU0548 0.13 +0.9 618,449 + livH DVU0548 0.25 +0.9 618,464 + livH DVU0548 0.27 +1.6 618,464 + livH DVU0548 0.27 +0.5 618,501 - livH DVU0548 0.31 +1.3 618,521 - livH DVU0548 0.33 +1.2 618,588 - livH DVU0548 0.41 +0.6 618,699 + livH DVU0548 0.53 +1.4 618,706 + livH DVU0548 0.54 +1.6 618,716 - livH DVU0548 0.55 +1.3 618,722 + livH DVU0548 0.55 +1.2 618,739 + livH DVU0548 0.57 +1.7 618,744 + livH DVU0548 0.58 +1.1 618,747 - livH DVU0548 0.58 +0.8 618,747 - livH DVU0548 0.58 +1.4 618,747 - livH DVU0548 0.58 +1.0 618,777 + livH DVU0548 0.61 -2.5 618,801 + livH DVU0548 0.64 +0.7 618,815 - livH DVU0548 0.66 +1.4 618,924 - livH DVU0548 0.78 +0.8 619,060 + +0.9 619,113 - +1.2 619,245 + +1.1 619,361 - DVU0549 0.17 +2.1 619,457 + DVU0549 0.25 +1.0 619,471 + DVU0549 0.26 +1.1 619,518 - DVU0549 0.30 +1.7 619,518 - DVU0549 0.30 +2.3 619,615 - DVU0549 0.38 +0.9 619,616 - DVU0549 0.38 +0.6 619,660 - DVU0549 0.41 +0.8 619,660 - DVU0549 0.41 +0.6 619,678 - DVU0549 0.43 +2.3 619,717 + DVU0549 0.46 +0.5 619,794 + DVU0549 0.52 +0.6 619,877 + DVU0549 0.59 -1.7 619,965 + DVU0549 0.66 +1.0 620,000 + DVU0549 0.69 +1.9 620,007 + DVU0549 0.70 -3.3 620,091 + DVU0549 0.77 +1.2 620,095 - DVU0549 0.77 +0.9 620,100 - DVU0549 0.77 +1.1 620,184 + DVU0549 0.84 +0.7 620,192 - DVU0549 0.85 +0.8 620,322 + +0.3 620,455 - +1.1 620,459 + livG DVU0550 0.10 +1.7 620,481 + livG DVU0550 0.13 +0.0 620,667 + livG DVU0550 0.37 +1.2 620,797 + livG DVU0550 0.53 +1.2 620,826 + livG DVU0550 0.57 +0.6 620,850 + livG DVU0550 0.60 +0.4 620,850 + livG DVU0550 0.60 -2.3 620,850 + livG DVU0550 0.60 +1.3 620,858 - livG DVU0550 0.61 +0.6 620,858 - livG DVU0550 0.61 +1.4 620,858 - livG DVU0550 0.61 +2.0 620,916 - livG DVU0550 0.68 +0.7 621,000 + livG DVU0550 0.79 +0.6 621,044 - livG DVU0550 0.85 +1.1 621,098 + +1.1 621,101 - +1.3 621,130 + -2.8 621,167 - -2.1 621,167 - -2.7 621,177 - +0.8 621,177 - -2.0 621,221 - +0.0 621,280 - livF DVU0551 0.17 +0.9 621,280 - livF DVU0551 0.17 +0.4 621,287 - livF DVU0551 0.18 +0.0 621,311 + livF DVU0551 0.22 +1.3 621,321 + livF DVU0551 0.23 +1.2
Or see this region's nucleotide sequence