Experiment: MoLS4 with L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glpK and mdaB are separated by 262 nucleotides mdaB and DVU3136 are separated by 55 nucleotides DVU3136 and fabG are separated by 627 nucleotides
DVU3134: glpK - glycerol kinase (TIGR), at 3,281,017 to 3,282,510
glpK
DVU3135: mdaB - flavodoxin-like fold domain protein (TIGR), at 3,282,773 to 3,283,381
mdaB
DVU3136: DVU3136 - nitroreductase family protein (TIGR), at 3,283,437 to 3,284,093
DVU3136
DVU3137: fabG - oxidoreductase, short chain dehydrogenase/reductase family (TIGR), at 3,284,721 to 3,285,497
fabG
Position (kb)
3283
3284
3285 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3282.492 kb on + strand at 3282.543 kb on - strand at 3282.599 kb on + strand at 3282.599 kb on + strand at 3282.599 kb on + strand at 3282.609 kb on - strand at 3282.747 kb on + strand at 3282.755 kb on - strand at 3282.813 kb on + strand at 3282.865 kb on + strand, within mdaB at 3282.935 kb on - strand, within mdaB at 3282.943 kb on + strand, within mdaB at 3283.085 kb on + strand, within mdaB at 3283.105 kb on - strand, within mdaB at 3283.131 kb on - strand, within mdaB at 3283.176 kb on + strand, within mdaB at 3283.180 kb on - strand, within mdaB at 3283.181 kb on + strand, within mdaB at 3283.214 kb on + strand, within mdaB at 3283.269 kb on - strand, within mdaB at 3283.420 kb on + strand at 3283.463 kb on - strand at 3283.466 kb on - strand at 3283.511 kb on - strand, within DVU3136 at 3283.533 kb on - strand, within DVU3136 at 3283.586 kb on + strand, within DVU3136 at 3283.608 kb on + strand, within DVU3136 at 3283.608 kb on + strand, within DVU3136 at 3283.610 kb on + strand, within DVU3136 at 3283.616 kb on - strand, within DVU3136 at 3283.644 kb on - strand, within DVU3136 at 3283.665 kb on - strand, within DVU3136 at 3283.680 kb on + strand, within DVU3136 at 3283.699 kb on + strand, within DVU3136 at 3283.705 kb on - strand, within DVU3136 at 3283.709 kb on + strand, within DVU3136 at 3283.717 kb on - strand, within DVU3136 at 3283.895 kb on + strand, within DVU3136 at 3283.955 kb on + strand, within DVU3136 at 3284.108 kb on + strand at 3284.178 kb on + strand at 3284.462 kb on - strand at 3284.468 kb on + strand at 3284.514 kb on + strand at 3284.641 kb on + strand at 3284.663 kb on + strand at 3284.790 kb on + strand at 3284.791 kb on - strand at 3284.791 kb on - strand at 3284.791 kb on - strand at 3284.798 kb on - strand at 3284.864 kb on - strand, within fabG at 3284.875 kb on + strand, within fabG at 3284.875 kb on + strand, within fabG at 3284.875 kb on + strand, within fabG at 3284.878 kb on - strand, within fabG at 3284.890 kb on - strand, within fabG at 3284.891 kb on - strand, within fabG at 3284.913 kb on + strand, within fabG at 3284.918 kb on + strand, within fabG at 3284.926 kb on - strand, within fabG at 3285.007 kb on - strand, within fabG at 3285.049 kb on - strand, within fabG at 3285.066 kb on - strand, within fabG at 3285.073 kb on - strand, within fabG at 3285.087 kb on + strand, within fabG at 3285.087 kb on + strand, within fabG at 3285.092 kb on + strand, within fabG
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glutamine (N) remove 3,282,492 + -1.6 3,282,543 - -0.6 3,282,599 + +0.2 3,282,599 + +0.1 3,282,599 + -3.2 3,282,609 - -0.0 3,282,747 + -0.3 3,282,755 - -1.7 3,282,813 + +0.4 3,282,865 + mdaB DVU3135 0.15 +0.2 3,282,935 - mdaB DVU3135 0.27 +0.0 3,282,943 + mdaB DVU3135 0.28 +0.6 3,283,085 + mdaB DVU3135 0.51 -3.1 3,283,105 - mdaB DVU3135 0.55 -0.1 3,283,131 - mdaB DVU3135 0.59 +0.1 3,283,176 + mdaB DVU3135 0.66 +0.5 3,283,180 - mdaB DVU3135 0.67 -0.2 3,283,181 + mdaB DVU3135 0.67 +0.8 3,283,214 + mdaB DVU3135 0.72 +0.4 3,283,269 - mdaB DVU3135 0.81 -4.5 3,283,420 + -0.1 3,283,463 - +0.8 3,283,466 - -0.0 3,283,511 - DVU3136 0.11 +0.4 3,283,533 - DVU3136 0.15 +0.1 3,283,586 + DVU3136 0.23 -0.5 3,283,608 + DVU3136 0.26 -0.3 3,283,608 + DVU3136 0.26 -0.3 3,283,610 + DVU3136 0.26 -1.7 3,283,616 - DVU3136 0.27 +1.0 3,283,644 - DVU3136 0.32 -1.6 3,283,665 - DVU3136 0.35 +0.6 3,283,680 + DVU3136 0.37 +0.1 3,283,699 + DVU3136 0.40 -2.2 3,283,705 - DVU3136 0.41 -4.2 3,283,709 + DVU3136 0.41 -3.0 3,283,717 - DVU3136 0.43 -0.0 3,283,895 + DVU3136 0.70 -3.0 3,283,955 + DVU3136 0.79 -0.0 3,284,108 + -0.1 3,284,178 + -0.1 3,284,462 - -0.7 3,284,468 + -0.1 3,284,514 + +0.5 3,284,641 + +0.1 3,284,663 + +0.1 3,284,790 + -2.9 3,284,791 - -0.4 3,284,791 - -0.7 3,284,791 - +0.8 3,284,798 - +0.4 3,284,864 - fabG DVU3137 0.18 +0.1 3,284,875 + fabG DVU3137 0.20 +0.2 3,284,875 + fabG DVU3137 0.20 +0.2 3,284,875 + fabG DVU3137 0.20 +1.8 3,284,878 - fabG DVU3137 0.20 -2.2 3,284,890 - fabG DVU3137 0.22 +0.2 3,284,891 - fabG DVU3137 0.22 -0.6 3,284,913 + fabG DVU3137 0.25 -0.2 3,284,918 + fabG DVU3137 0.25 +0.4 3,284,926 - fabG DVU3137 0.26 -0.3 3,285,007 - fabG DVU3137 0.37 +0.3 3,285,049 - fabG DVU3137 0.42 +0.2 3,285,066 - fabG DVU3137 0.44 +0.1 3,285,073 - fabG DVU3137 0.45 -0.3 3,285,087 + fabG DVU3137 0.47 +0.0 3,285,087 + fabG DVU3137 0.47 -2.7 3,285,092 + fabG DVU3137 0.48 -1.7
Or see this region's nucleotide sequence