Experiment: L-Malic Acid (C) with ammonium chloride; high light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1322 and Rru_A1323 are separated by 17 nucleotides Rru_A1323 and Rru_A1324 are separated by 13 nucleotides Rru_A1324 and Rru_A1325 are separated by 41 nucleotides Rru_A1325 and Rru_A1326 are separated by 33 nucleotides
Rru_A1322: Rru_A1322 - alpha subunit of malonate decarboxylase (NCBI), at 1,560,682 to 1,562,355
_A1322
Rru_A1323: Rru_A1323 - Malonate decarboxylase delta subunit (NCBI), at 1,562,373 to 1,562,690
_A1323
Rru_A1324: Rru_A1324 - beta subunit of malonate decarboxylase (NCBI), at 1,562,704 to 1,563,660
_A1324
Rru_A1325: Rru_A1325 - hypothetical protein (NCBI), at 1,563,702 to 1,564,595
_A1325
Rru_A1326: Rru_A1326 - hypothetical protein (NCBI), at 1,564,629 to 1,565,729
_A1326
Position (kb)
1562
1563
1564 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1561.720 kb on + strand, within Rru_A1322 at 1561.720 kb on + strand, within Rru_A1322 at 1561.720 kb on + strand, within Rru_A1322 at 1561.721 kb on - strand, within Rru_A1322 at 1562.054 kb on - strand, within Rru_A1322 at 1562.054 kb on - strand, within Rru_A1322 at 1562.098 kb on + strand, within Rru_A1322 at 1562.098 kb on + strand, within Rru_A1322 at 1562.098 kb on + strand, within Rru_A1322 at 1562.098 kb on + strand, within Rru_A1322 at 1562.099 kb on - strand, within Rru_A1322 at 1562.323 kb on + strand at 1562.323 kb on + strand at 1562.324 kb on - strand at 1562.324 kb on - strand at 1562.356 kb on + strand at 1562.356 kb on + strand at 1562.688 kb on + strand at 1562.688 kb on + strand at 1562.688 kb on + strand at 1562.704 kb on - strand at 1563.376 kb on + strand, within Rru_A1324 at 1563.376 kb on + strand, within Rru_A1324 at 1563.376 kb on + strand, within Rru_A1324 at 1563.376 kb on + strand, within Rru_A1324 at 1563.376 kb on + strand, within Rru_A1324 at 1563.377 kb on - strand, within Rru_A1324 at 1563.377 kb on - strand, within Rru_A1324 at 1563.377 kb on - strand, within Rru_A1324 at 1563.377 kb on - strand, within Rru_A1324 at 1563.377 kb on - strand, within Rru_A1324 at 1563.394 kb on - strand, within Rru_A1324 at 1563.394 kb on - strand, within Rru_A1324 at 1563.394 kb on - strand, within Rru_A1324 at 1563.397 kb on + strand, within Rru_A1324 at 1563.398 kb on - strand, within Rru_A1324 at 1563.398 kb on - strand, within Rru_A1324 at 1563.398 kb on - strand, within Rru_A1324 at 1563.677 kb on + strand at 1563.678 kb on - strand at 1563.782 kb on + strand at 1563.783 kb on - strand at 1563.918 kb on + strand, within Rru_A1325 at 1563.918 kb on + strand, within Rru_A1325 at 1563.918 kb on + strand, within Rru_A1325 at 1563.919 kb on - strand, within Rru_A1325 at 1563.948 kb on + strand, within Rru_A1325 at 1563.948 kb on + strand, within Rru_A1325 at 1563.948 kb on + strand, within Rru_A1325 at 1563.949 kb on - strand, within Rru_A1325 at 1563.949 kb on - strand, within Rru_A1325 at 1563.969 kb on + strand, within Rru_A1325 at 1563.970 kb on - strand, within Rru_A1325 at 1563.970 kb on - strand, within Rru_A1325 at 1563.970 kb on - strand, within Rru_A1325 at 1564.101 kb on + strand, within Rru_A1325 at 1564.102 kb on - strand, within Rru_A1325 at 1564.578 kb on + strand at 1564.579 kb on - strand at 1564.601 kb on + strand at 1564.602 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) with ammonium chloride; high light remove 1,561,720 + Rru_A1322 0.62 -0.2 1,561,720 + Rru_A1322 0.62 -0.2 1,561,720 + Rru_A1322 0.62 -0.2 1,561,721 - Rru_A1322 0.62 -0.2 1,562,054 - Rru_A1322 0.82 -0.2 1,562,054 - Rru_A1322 0.82 -0.2 1,562,098 + Rru_A1322 0.85 -0.2 1,562,098 + Rru_A1322 0.85 -0.4 1,562,098 + Rru_A1322 0.85 -0.2 1,562,098 + Rru_A1322 0.85 -0.2 1,562,099 - Rru_A1322 0.85 -0.2 1,562,323 + -0.7 1,562,323 + -1.1 1,562,324 - -0.2 1,562,324 - -0.2 1,562,356 + -2.5 1,562,356 + -3.4 1,562,688 + -0.6 1,562,688 + +0.2 1,562,688 + -1.5 1,562,704 - -2.9 1,563,376 + Rru_A1324 0.70 -1.8 1,563,376 + Rru_A1324 0.70 -2.0 1,563,376 + Rru_A1324 0.70 -1.8 1,563,376 + Rru_A1324 0.70 -1.2 1,563,376 + Rru_A1324 0.70 -0.9 1,563,377 - Rru_A1324 0.70 -0.2 1,563,377 - Rru_A1324 0.70 -0.2 1,563,377 - Rru_A1324 0.70 -1.0 1,563,377 - Rru_A1324 0.70 -0.2 1,563,377 - Rru_A1324 0.70 -0.2 1,563,394 - Rru_A1324 0.72 -0.2 1,563,394 - Rru_A1324 0.72 +0.1 1,563,394 - Rru_A1324 0.72 -0.2 1,563,397 + Rru_A1324 0.72 -0.2 1,563,398 - Rru_A1324 0.73 -1.8 1,563,398 - Rru_A1324 0.73 +0.1 1,563,398 - Rru_A1324 0.73 -0.2 1,563,677 + -3.2 1,563,678 - -0.2 1,563,782 + -1.2 1,563,783 - -1.2 1,563,918 + Rru_A1325 0.24 -0.2 1,563,918 + Rru_A1325 0.24 -1.3 1,563,918 + Rru_A1325 0.24 -0.2 1,563,919 - Rru_A1325 0.24 -0.2 1,563,948 + Rru_A1325 0.28 -0.4 1,563,948 + Rru_A1325 0.28 -1.2 1,563,948 + Rru_A1325 0.28 -1.3 1,563,949 - Rru_A1325 0.28 -0.2 1,563,949 - Rru_A1325 0.28 -0.2 1,563,969 + Rru_A1325 0.30 -0.2 1,563,970 - Rru_A1325 0.30 -0.2 1,563,970 - Rru_A1325 0.30 -1.7 1,563,970 - Rru_A1325 0.30 -0.2 1,564,101 + Rru_A1325 0.45 -0.2 1,564,102 - Rru_A1325 0.45 +0.0 1,564,578 + -0.2 1,564,579 - -1.2 1,564,601 + -0.2 1,564,602 - -1.7
Or see this region's nucleotide sequence