Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3809 and Shew_3810 are separated by 21 nucleotides Shew_3810 and Shew_3811 are separated by 10 nucleotides Shew_3811 and Shew_R0123 are separated by 702 nucleotides Shew_R0123 and Shew_R0124 are separated by 99 nucleotides
Shew_3809: Shew_3809 - protein of unknown function DUF610, YibQ (RefSeq), at 4,532,857 to 4,533,612
_3809
Shew_3810: Shew_3810 - BadM/Rrf2 family transcriptional regulator (RefSeq), at 4,533,634 to 4,534,086
_3810
Shew_3811: Shew_3811 - hypothetical protein (RefSeq), at 4,534,097 to 4,534,351
_3811
Shew_R0123: Shew_R0123 - 5S ribosomal RNA (RefSeq), at 4,535,054 to 4,535,174
_R0123
Shew_R0124: Shew_R0124 - 23S ribosomal RNA (RefSeq), at 4,535,274 to 4,538,166
_R0124
Position (kb)
4534
4535 Strain fitness (log2 ratio)
-2
-1
0
1 at 4533.372 kb on + strand, within Shew_3809 at 4533.465 kb on + strand, within Shew_3809 at 4533.473 kb on - strand, within Shew_3809 at 4533.674 kb on + strand at 4533.764 kb on + strand, within Shew_3810 at 4533.772 kb on - strand, within Shew_3810 at 4533.950 kb on + strand, within Shew_3810 at 4534.130 kb on - strand, within Shew_3811 at 4534.299 kb on - strand, within Shew_3811 at 4534.431 kb on - strand at 4534.464 kb on + strand at 4534.501 kb on - strand at 4534.698 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 4,533,372 + Shew_3809 0.68 -0.0 4,533,465 + Shew_3809 0.80 -0.8 4,533,473 - Shew_3809 0.81 -0.7 4,533,674 + -0.7 4,533,764 + Shew_3810 0.29 +0.0 4,533,772 - Shew_3810 0.30 +0.2 4,533,950 + Shew_3810 0.70 -0.1 4,534,130 - Shew_3811 0.13 -1.8 4,534,299 - Shew_3811 0.79 -0.8 4,534,431 - -0.7 4,534,464 + -0.4 4,534,501 - +0.2 4,534,698 - -0.2
Or see this region's nucleotide sequence