Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2062 and Shew_2063 overlap by 7 nucleotides Shew_2063 and Shew_2064 are separated by 126 nucleotides Shew_2064 and Shew_R0074 are separated by 744 nucleotides Shew_R0074 and Shew_R0075 are separated by 42 nucleotides Shew_R0075 and Shew_R0076 are separated by 26 nucleotides
Shew_2062: Shew_2062 - exsB protein (RefSeq), at 2,381,452 to 2,382,135
_2062
Shew_2063: Shew_2063 - rhomboid family protein (RefSeq), at 2,382,129 to 2,382,713
_2063
Shew_2064: Shew_2064 - excinuclease ABC subunit B (RefSeq), at 2,382,840 to 2,384,858
_2064
Shew_R0074: Shew_R0074 - tRNA-Asn (RefSeq), at 2,385,603 to 2,385,678
_R0074
Shew_R0075: Shew_R0075 - tRNA-Asn (RefSeq), at 2,385,721 to 2,385,796
_R0075
Shew_R0076: Shew_R0076 - tRNA-Asn (RefSeq), at 2,385,823 to 2,385,898
_R0076
Position (kb)
2382
2383
2384
2385 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2381.978 kb on + strand, within Shew_2062 at 2382.216 kb on - strand, within Shew_2063 at 2382.559 kb on + strand, within Shew_2063 at 2383.002 kb on - strand at 2383.212 kb on + strand, within Shew_2064 at 2383.341 kb on - strand, within Shew_2064 at 2383.806 kb on - strand, within Shew_2064 at 2384.126 kb on + strand, within Shew_2064 at 2384.281 kb on - strand, within Shew_2064 at 2384.868 kb on + strand at 2384.876 kb on - strand at 2385.063 kb on - strand at 2385.307 kb on + strand at 2385.371 kb on + strand at 2385.393 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 2,381,978 + Shew_2062 0.77 -0.7 2,382,216 - Shew_2063 0.15 -3.7 2,382,559 + Shew_2063 0.74 -2.6 2,383,002 - -0.3 2,383,212 + Shew_2064 0.18 -0.2 2,383,341 - Shew_2064 0.25 -0.3 2,383,806 - Shew_2064 0.48 -0.2 2,384,126 + Shew_2064 0.64 -1.9 2,384,281 - Shew_2064 0.71 -1.0 2,384,868 + +0.3 2,384,876 - +0.0 2,385,063 - +0.3 2,385,307 + +0.6 2,385,371 + -0.5 2,385,393 + +0.1
Or see this region's nucleotide sequence