Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2059 and Shew_R0071 are separated by 125 nucleotides Shew_R0071 and Shew_R0072 are separated by 31 nucleotides Shew_R0072 and Shew_R0073 are separated by 40 nucleotides Shew_R0073 and Shew_2060 are separated by 293 nucleotides Shew_2060 and Shew_2061 are separated by 141 nucleotides Shew_2061 and Shew_2062 are separated by 184 nucleotides
Shew_2059: Shew_2059 - hypothetical protein (RefSeq), at 2,378,163 to 2,378,651
_2059
Shew_R0071: Shew_R0071 - tRNA-Tyr (RefSeq), at 2,378,777 to 2,378,861
_R0071
Shew_R0072: Shew_R0072 - tRNA-Tyr (RefSeq), at 2,378,893 to 2,378,977
_R0072
Shew_R0073: Shew_R0073 - tRNA-Tyr (RefSeq), at 2,379,018 to 2,379,102
_R0073
Shew_2060: Shew_2060 - pyridoxal-5'-phosphate-dependent enzyme, beta subunit (RefSeq), at 2,379,396 to 2,380,457
_2060
Shew_2061: Shew_2061 - radical SAM domain-containing protein (RefSeq), at 2,380,599 to 2,381,267
_2061
Shew_2062: Shew_2062 - exsB protein (RefSeq), at 2,381,452 to 2,382,135
_2062
Position (kb)
2379
2380
2381 Strain fitness (log2 ratio)
-2
-1
0
1 at 2378.499 kb on - strand, within Shew_2059 at 2378.569 kb on - strand, within Shew_2059 at 2379.431 kb on + strand at 2379.908 kb on + strand, within Shew_2060 at 2381.019 kb on + strand, within Shew_2061 at 2381.118 kb on + strand, within Shew_2061 at 2381.331 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 2,378,499 - Shew_2059 0.69 +0.3 2,378,569 - Shew_2059 0.83 -0.6 2,379,431 + -2.6 2,379,908 + Shew_2060 0.48 -0.8 2,381,019 + Shew_2061 0.63 +0.8 2,381,118 + Shew_2061 0.78 +0.8 2,381,331 - +0.5
Or see this region's nucleotide sequence