Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1988 and Shew_1989 are separated by 110 nucleotides Shew_1989 and Shew_1990 are separated by 6 nucleotides Shew_1990 and Shew_1991 overlap by 4 nucleotides Shew_1991 and Shew_1992 overlap by 4 nucleotides Shew_1992 and Shew_1993 are separated by 55 nucleotides
Shew_1988: Shew_1988 - cytochrome c oxidase, cbb3-type, subunit III (RefSeq), at 2,299,988 to 2,300,959
_1988
Shew_1989: Shew_1989 - hypothetical protein (RefSeq), at 2,301,070 to 2,301,549
_1989
Shew_1990: Shew_1990 - heavy metal translocating P-type ATPase (RefSeq), at 2,301,556 to 2,303,979
_1990
Shew_1991: Shew_1991 - cytochrome oxidase maturation protein, cbb3-type (RefSeq), at 2,303,976 to 2,304,161
_1991
Shew_1992: Shew_1992 - hypothetical protein (RefSeq), at 2,304,158 to 2,304,850
_1992
Shew_1993: Shew_1993 - fumarate/nitrate reduction transcriptional regulator (RefSeq), at 2,304,906 to 2,305,655
_1993
Position (kb)
2301
2302
2303
2304 Strain fitness (log2 ratio)
-2
-1
0
1 at 2301.326 kb on - strand, within Shew_1989 at 2302.498 kb on - strand, within Shew_1990 at 2303.529 kb on - strand, within Shew_1990 at 2304.173 kb on - strand at 2304.475 kb on + strand, within Shew_1992 at 2304.475 kb on + strand, within Shew_1992 at 2304.488 kb on - strand, within Shew_1992 at 2304.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 2,301,326 - Shew_1989 0.53 -0.5 2,302,498 - Shew_1990 0.39 -0.9 2,303,529 - Shew_1990 0.81 -0.7 2,304,173 - -1.3 2,304,475 + Shew_1992 0.46 -1.1 2,304,475 + Shew_1992 0.46 -0.8 2,304,488 - Shew_1992 0.48 -0.7 2,304,944 - -2.1
Or see this region's nucleotide sequence