Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1527 and Shew_1528 are separated by 34 nucleotides Shew_1528 and Shew_1529 are separated by 19 nucleotides Shew_1529 and Shew_R0050 are separated by 159 nucleotides Shew_R0050 and Shew_R0051 are separated by 38 nucleotides Shew_R0051 and Shew_R0052 are separated by 38 nucleotides Shew_R0052 and Shew_R0053 are separated by 174 nucleotides Shew_R0053 and Shew_R0054 are separated by 174 nucleotides
Shew_1527: Shew_1527 - TolB domain-containing protein (RefSeq), at 1,750,081 to 1,751,406
_1527
Shew_1528: Shew_1528 - OmpA domain-containing protein (RefSeq), at 1,751,441 to 1,751,983
_1528
Shew_1529: Shew_1529 - TPR repeat-containing protein (RefSeq), at 1,752,003 to 1,752,725
_1529
Shew_R0050: Shew_R0050 - tRNA-Lys (RefSeq), at 1,752,885 to 1,752,960
_R0050
Shew_R0051: Shew_R0051 - tRNA-Lys (RefSeq), at 1,752,999 to 1,753,074
_R0051
Shew_R0052: Shew_R0052 - tRNA-Lys (RefSeq), at 1,753,113 to 1,753,188
_R0052
Shew_R0053: Shew_R0053 - tRNA-Lys (RefSeq), at 1,753,363 to 1,753,438
_R0053
Shew_R0054: Shew_R0054 - tRNA-Lys (RefSeq), at 1,753,613 to 1,753,688
_R0054
Position (kb)
1752
1753 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1751.140 kb on - strand, within Shew_1527 at 1751.255 kb on + strand, within Shew_1527 at 1751.601 kb on + strand, within Shew_1528 at 1751.984 kb on + strand at 1752.201 kb on - strand, within Shew_1529 at 1752.334 kb on + strand, within Shew_1529 at 1752.334 kb on + strand, within Shew_1529 at 1752.547 kb on - strand, within Shew_1529
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 1,751,140 - Shew_1527 0.80 +3.1 1,751,255 + Shew_1527 0.89 +1.9 1,751,601 + Shew_1528 0.29 +1.7 1,751,984 + -0.4 1,752,201 - Shew_1529 0.27 -1.1 1,752,334 + Shew_1529 0.46 -0.5 1,752,334 + Shew_1529 0.46 -0.8 1,752,547 - Shew_1529 0.75 -0.4
Or see this region's nucleotide sequence