Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1431 and Shew_1432 are separated by 110 nucleotides Shew_1432 and Shew_1433 are separated by 44 nucleotides Shew_1433 and Shew_1434 overlap by 1 nucleotides
Shew_1431: Shew_1431 - hypothetical protein (RefSeq), at 1,646,533 to 1,646,988
_1431
Shew_1432: Shew_1432 - ferredoxin-type protein NapF (RefSeq), at 1,647,099 to 1,647,575
_1432
Shew_1433: Shew_1433 - UDP-glucose 4-epimerase (RefSeq), at 1,647,620 to 1,648,633
_1433
Shew_1434: Shew_1434 - UTP-glucose-1-phosphate uridylyltransferase GalU (RefSeq), at 1,648,633 to 1,649,553
_1434
Position (kb)
1647
1648
1649 Strain fitness (log2 ratio)
-2
-1
0
1 at 1646.698 kb on + strand, within Shew_1431 at 1646.901 kb on - strand, within Shew_1431 at 1646.901 kb on - strand, within Shew_1431 at 1647.279 kb on - strand, within Shew_1432 at 1647.640 kb on + strand at 1647.759 kb on + strand, within Shew_1433 at 1647.834 kb on + strand, within Shew_1433 at 1647.847 kb on - strand, within Shew_1433 at 1648.313 kb on - strand, within Shew_1433 at 1648.315 kb on + strand, within Shew_1433 at 1648.315 kb on + strand, within Shew_1433 at 1648.323 kb on - strand, within Shew_1433 at 1648.585 kb on - strand at 1648.637 kb on + strand at 1649.220 kb on - strand, within Shew_1434
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 1,646,698 + Shew_1431 0.36 +0.3 1,646,901 - Shew_1431 0.81 -2.5 1,646,901 - Shew_1431 0.81 -0.7 1,647,279 - Shew_1432 0.38 +0.7 1,647,640 + +0.9 1,647,759 + Shew_1433 0.14 -1.0 1,647,834 + Shew_1433 0.21 -0.8 1,647,847 - Shew_1433 0.22 -0.9 1,648,313 - Shew_1433 0.68 -0.8 1,648,315 + Shew_1433 0.69 -1.4 1,648,315 + Shew_1433 0.69 -0.5 1,648,323 - Shew_1433 0.69 -0.1 1,648,585 - -0.9 1,648,637 + -1.0 1,649,220 - Shew_1434 0.64 -0.3
Or see this region's nucleotide sequence