Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0658 and Shew_0659 are separated by 105 nucleotides Shew_0659 and Shew_0660 overlap by 4 nucleotides Shew_0660 and Shew_0661 are separated by 70 nucleotides
Shew_0658: Shew_0658 - lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase (RefSeq), at 748,289 to 749,215
_0658
Shew_0659: Shew_0659 - bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (RefSeq), at 749,321 to 750,751
_0659
Shew_0660: Shew_0660 - hypothetical protein (RefSeq), at 750,748 to 751,584
_0660
Shew_0661: Shew_0661 - hypothetical protein (RefSeq), at 751,655 to 751,969
_0661
Position (kb) 749
750
751 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 748.820 kb on + strand, within Shew_0658 at 748.946 kb on - strand, within Shew_0658 at 749.400 kb on + strand at 749.639 kb on - strand, within Shew_0659 at 749.832 kb on - strand, within Shew_0659 at 750.227 kb on - strand, within Shew_0659 at 750.302 kb on + strand, within Shew_0659 at 750.811 kb on + strand at 750.819 kb on - strand at 751.026 kb on + strand, within Shew_0660 at 751.034 kb on - strand, within Shew_0660 at 751.178 kb on + strand, within Shew_0660 at 751.185 kb on + strand, within Shew_0660 at 751.238 kb on + strand, within Shew_0660 at 751.246 kb on - strand, within Shew_0660 at 751.246 kb on - strand, within Shew_0660 at 751.246 kb on - strand, within Shew_0660 at 751.370 kb on + strand, within Shew_0660 at 751.524 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 748,820 + Shew_0658 0.57 -0.9 748,946 - Shew_0658 0.71 +2.5 749,400 + -1.8 749,639 - Shew_0659 0.22 -1.2 749,832 - Shew_0659 0.36 -1.3 750,227 - Shew_0659 0.63 -3.8 750,302 + Shew_0659 0.69 -1.7 750,811 + -1.0 750,819 - +0.0 751,026 + Shew_0660 0.33 -0.1 751,034 - Shew_0660 0.34 +0.1 751,178 + Shew_0660 0.51 +0.4 751,185 + Shew_0660 0.52 -0.3 751,238 + Shew_0660 0.59 +0.2 751,246 - Shew_0660 0.59 -0.2 751,246 - Shew_0660 0.59 -0.1 751,246 - Shew_0660 0.59 -0.1 751,370 + Shew_0660 0.74 +0.5 751,524 + +0.3
Or see this region's nucleotide sequence