Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0620 and Shew_0621 are separated by 80 nucleotides Shew_0621 and Shew_0622 are separated by 60 nucleotides Shew_0622 and Shew_0623 are separated by 207 nucleotides Shew_0623 and Shew_0624 are separated by 70 nucleotides
Shew_0620: Shew_0620 - ion transport 2 domain-containing protein (RefSeq), at 705,768 to 706,562
_0620
Shew_0621: Shew_0621 - hypothetical protein (RefSeq), at 706,643 to 706,975
_0621
Shew_0622: Shew_0622 - hypothetical protein (RefSeq), at 707,036 to 707,680
_0622
Shew_0623: Shew_0623 - phage shock protein A, PspA (RefSeq), at 707,888 to 708,574
_0623
Shew_0624: Shew_0624 - hypothetical protein (RefSeq), at 708,645 to 709,058
_0624
Position (kb) 707
708 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 706.222 kb on - strand, within Shew_0620 at 706.768 kb on - strand, within Shew_0621 at 706.790 kb on - strand, within Shew_0621 at 706.831 kb on - strand, within Shew_0621 at 706.990 kb on - strand at 707.029 kb on + strand at 707.029 kb on + strand at 707.111 kb on - strand, within Shew_0622 at 707.495 kb on - strand, within Shew_0622 at 707.848 kb on + strand at 707.917 kb on - strand at 708.203 kb on - strand, within Shew_0623
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 706,222 - Shew_0620 0.57 -0.3 706,768 - Shew_0621 0.38 -2.8 706,790 - Shew_0621 0.44 -0.8 706,831 - Shew_0621 0.56 -1.0 706,990 - -1.1 707,029 + -0.9 707,029 + -1.1 707,111 - Shew_0622 0.12 -1.5 707,495 - Shew_0622 0.71 -1.8 707,848 + -2.6 707,917 - -2.3 708,203 - Shew_0623 0.46 -2.4
Or see this region's nucleotide sequence