Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0223 and Shew_0224 are separated by 21 nucleotides Shew_0224 and Shew_0225 overlap by 4 nucleotides Shew_0225 and Shew_0226 are separated by 68 nucleotides Shew_0226 and Shew_0227 overlap by 4 nucleotides
Shew_0223: Shew_0223 - hypothetical protein (RefSeq), at 237,090 to 237,959
_0223
Shew_0224: Shew_0224 - transport-associated (RefSeq), at 237,981 to 238,550
_0224
Shew_0225: Shew_0225 - phosphoheptose isomerase (RefSeq), at 238,547 to 239,140
_0225
Shew_0226: Shew_0226 - hypothetical protein (RefSeq), at 239,209 to 239,553
_0226
Shew_0227: Shew_0227 - LppC family lipoprotein (RefSeq), at 239,550 to 241,454
_0227
Position (kb) 238
239
240 Strain fitness (log2 ratio) -1
0
1
2
3 at 237.693 kb on + strand, within Shew_0223 at 237.693 kb on + strand, within Shew_0223 at 237.705 kb on + strand, within Shew_0223 at 237.711 kb on - strand, within Shew_0223 at 237.768 kb on + strand, within Shew_0223 at 238.094 kb on - strand, within Shew_0224 at 238.167 kb on - strand, within Shew_0224 at 238.292 kb on + strand, within Shew_0224 at 238.300 kb on - strand, within Shew_0224 at 239.042 kb on + strand, within Shew_0225 at 239.254 kb on - strand, within Shew_0226 at 239.495 kb on - strand, within Shew_0226 at 240.078 kb on + strand, within Shew_0227
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 237,693 + Shew_0223 0.69 -1.5 237,693 + Shew_0223 0.69 +0.8 237,705 + Shew_0223 0.71 -0.4 237,711 - Shew_0223 0.71 -0.8 237,768 + Shew_0223 0.78 +0.1 238,094 - Shew_0224 0.20 +0.1 238,167 - Shew_0224 0.33 +0.5 238,292 + Shew_0224 0.55 +0.2 238,300 - Shew_0224 0.56 +0.0 239,042 + Shew_0225 0.83 -0.6 239,254 - Shew_0226 0.13 -0.4 239,495 - Shew_0226 0.83 +0.1 240,078 + Shew_0227 0.28 +3.2
Or see this region's nucleotide sequence