Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4997 and Psyr_4998 are separated by 156 nucleotides Psyr_4998 and Psyr_4999 are separated by 63 nucleotides Psyr_4999 and Psyr_5000 are separated by 376 nucleotides Psyr_5000 and Psyr_5001 are separated by 77 nucleotides
Psyr_4997: Psyr_4997 - YD repeat protein, at 5,926,344 to 5,928,011
_4997
Psyr_4998: Psyr_4998 - hypothetical protein, at 5,928,168 to 5,928,878
_4998
Psyr_4999: Psyr_4999 - hypothetical protein, at 5,928,942 to 5,929,493
_4999
Psyr_5000: Psyr_5000 - conserved hypothetical protein, at 5,929,870 to 5,930,298
_5000
Psyr_5001: Psyr_5001 - conserved hypothetical protein, at 5,930,376 to 5,932,823
_5001
Position (kb) 5928
5929
5930 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5927.952 kb on + strand at 5927.953 kb on - strand at 5927.955 kb on + strand at 5927.955 kb on + strand at 5927.955 kb on + strand at 5927.955 kb on + strand at 5927.956 kb on - strand at 5927.956 kb on - strand at 5927.956 kb on - strand at 5927.956 kb on - strand at 5927.956 kb on - strand at 5927.956 kb on - strand at 5927.956 kb on - strand at 5927.977 kb on + strand at 5927.990 kb on + strand at 5927.991 kb on - strand at 5928.062 kb on - strand at 5928.062 kb on - strand at 5928.084 kb on + strand at 5928.085 kb on - strand at 5928.091 kb on - strand at 5928.095 kb on - strand at 5928.099 kb on - strand at 5928.103 kb on + strand at 5928.161 kb on + strand at 5928.232 kb on + strand at 5928.233 kb on - strand at 5928.277 kb on + strand, within Psyr_4998 at 5928.298 kb on + strand, within Psyr_4998 at 5928.313 kb on + strand, within Psyr_4998 at 5928.316 kb on - strand, within Psyr_4998 at 5928.316 kb on - strand, within Psyr_4998 at 5928.398 kb on + strand, within Psyr_4998 at 5928.398 kb on + strand, within Psyr_4998 at 5928.411 kb on + strand, within Psyr_4998 at 5928.412 kb on - strand, within Psyr_4998 at 5928.412 kb on - strand, within Psyr_4998 at 5928.416 kb on + strand, within Psyr_4998 at 5928.417 kb on - strand, within Psyr_4998 at 5928.510 kb on + strand, within Psyr_4998 at 5928.511 kb on - strand, within Psyr_4998 at 5928.557 kb on + strand, within Psyr_4998 at 5928.558 kb on - strand, within Psyr_4998 at 5928.567 kb on + strand, within Psyr_4998 at 5928.567 kb on + strand, within Psyr_4998 at 5928.568 kb on - strand, within Psyr_4998 at 5928.568 kb on - strand, within Psyr_4998 at 5928.570 kb on - strand, within Psyr_4998 at 5928.570 kb on - strand, within Psyr_4998 at 5928.583 kb on - strand, within Psyr_4998 at 5928.661 kb on + strand, within Psyr_4998 at 5928.661 kb on + strand, within Psyr_4998 at 5928.662 kb on - strand, within Psyr_4998 at 5928.683 kb on + strand, within Psyr_4998 at 5928.787 kb on - strand, within Psyr_4998 at 5928.787 kb on - strand, within Psyr_4998 at 5928.787 kb on - strand, within Psyr_4998 at 5928.798 kb on + strand, within Psyr_4998 at 5928.862 kb on + strand at 5928.862 kb on + strand at 5928.983 kb on + strand at 5928.983 kb on + strand at 5928.984 kb on - strand at 5928.984 kb on - strand at 5928.984 kb on - strand at 5928.984 kb on - strand at 5928.994 kb on - strand at 5929.026 kb on + strand, within Psyr_4999 at 5929.063 kb on + strand, within Psyr_4999 at 5929.063 kb on + strand, within Psyr_4999 at 5929.064 kb on - strand, within Psyr_4999 at 5929.064 kb on - strand, within Psyr_4999 at 5929.104 kb on + strand, within Psyr_4999 at 5929.104 kb on + strand, within Psyr_4999 at 5929.105 kb on - strand, within Psyr_4999 at 5929.159 kb on + strand, within Psyr_4999 at 5929.305 kb on + strand, within Psyr_4999 at 5929.305 kb on + strand, within Psyr_4999 at 5929.305 kb on + strand, within Psyr_4999 at 5929.305 kb on + strand, within Psyr_4999 at 5929.305 kb on + strand, within Psyr_4999 at 5929.306 kb on - strand, within Psyr_4999 at 5929.391 kb on + strand, within Psyr_4999 at 5929.491 kb on + strand at 5929.711 kb on - strand at 5929.712 kb on + strand at 5929.712 kb on + strand at 5929.712 kb on + strand at 5929.712 kb on + strand at 5929.712 kb on + strand at 5929.713 kb on - strand at 5929.713 kb on - strand at 5929.754 kb on + strand at 5929.754 kb on + strand at 5929.757 kb on + strand at 5929.757 kb on + strand at 5929.777 kb on + strand at 5929.777 kb on + strand at 5929.777 kb on + strand at 5929.777 kb on + strand at 5929.778 kb on - strand at 5929.778 kb on - strand at 5929.778 kb on - strand at 5929.778 kb on - strand at 5929.778 kb on - strand at 5929.778 kb on - strand at 5929.794 kb on + strand at 5929.863 kb on + strand at 5929.863 kb on + strand at 5929.863 kb on + strand at 5929.863 kb on + strand at 5929.863 kb on + strand at 5929.864 kb on - strand at 5929.864 kb on - strand at 5929.948 kb on + strand, within Psyr_5000 at 5929.948 kb on + strand, within Psyr_5000 at 5929.948 kb on + strand, within Psyr_5000 at 5929.948 kb on + strand, within Psyr_5000 at 5929.948 kb on + strand, within Psyr_5000 at 5929.948 kb on + strand, within Psyr_5000 at 5929.948 kb on + strand, within Psyr_5000 at 5929.948 kb on + strand, within Psyr_5000 at 5929.948 kb on + strand, within Psyr_5000 at 5929.948 kb on + strand, within Psyr_5000 at 5929.948 kb on + strand, within Psyr_5000 at 5929.949 kb on - strand, within Psyr_5000 at 5929.949 kb on - strand, within Psyr_5000 at 5929.949 kb on - strand, within Psyr_5000 at 5929.949 kb on - strand, within Psyr_5000 at 5929.949 kb on - strand, within Psyr_5000 at 5929.949 kb on - strand, within Psyr_5000 at 5929.949 kb on - strand, within Psyr_5000 at 5929.949 kb on - strand, within Psyr_5000 at 5929.949 kb on - strand, within Psyr_5000 at 5929.949 kb on - strand, within Psyr_5000 at 5930.010 kb on + strand, within Psyr_5000 at 5930.010 kb on + strand, within Psyr_5000 at 5930.011 kb on - strand, within Psyr_5000 at 5930.011 kb on - strand, within Psyr_5000 at 5930.011 kb on - strand, within Psyr_5000 at 5930.060 kb on - strand, within Psyr_5000 at 5930.060 kb on - strand, within Psyr_5000 at 5930.060 kb on - strand, within Psyr_5000 at 5930.133 kb on - strand, within Psyr_5000 at 5930.208 kb on + strand, within Psyr_5000 at 5930.208 kb on + strand, within Psyr_5000 at 5930.209 kb on - strand, within Psyr_5000 at 5930.209 kb on - strand, within Psyr_5000 at 5930.212 kb on + strand, within Psyr_5000 at 5930.212 kb on + strand, within Psyr_5000 at 5930.212 kb on + strand, within Psyr_5000 at 5930.212 kb on + strand, within Psyr_5000 at 5930.212 kb on + strand, within Psyr_5000 at 5930.213 kb on - strand, within Psyr_5000 at 5930.213 kb on - strand, within Psyr_5000 at 5930.213 kb on - strand, within Psyr_5000 at 5930.213 kb on - strand, within Psyr_5000 at 5930.213 kb on - strand, within Psyr_5000 at 5930.213 kb on - strand, within Psyr_5000 at 5930.230 kb on + strand, within Psyr_5000 at 5930.230 kb on + strand, within Psyr_5000 at 5930.230 kb on + strand, within Psyr_5000 at 5930.230 kb on + strand, within Psyr_5000 at 5930.230 kb on + strand, within Psyr_5000 at 5930.230 kb on + strand, within Psyr_5000 at 5930.230 kb on + strand, within Psyr_5000 at 5930.230 kb on + strand, within Psyr_5000 at 5930.231 kb on - strand, within Psyr_5000 at 5930.231 kb on - strand, within Psyr_5000 at 5930.236 kb on + strand, within Psyr_5000 at 5930.236 kb on + strand, within Psyr_5000 at 5930.236 kb on + strand, within Psyr_5000 at 5930.236 kb on + strand, within Psyr_5000 at 5930.236 kb on + strand, within Psyr_5000 at 5930.237 kb on - strand, within Psyr_5000 at 5930.237 kb on - strand, within Psyr_5000 at 5930.239 kb on + strand, within Psyr_5000 at 5930.239 kb on + strand, within Psyr_5000 at 5930.239 kb on + strand, within Psyr_5000 at 5930.240 kb on - strand, within Psyr_5000 at 5930.240 kb on - strand, within Psyr_5000 at 5930.240 kb on - strand, within Psyr_5000 at 5930.240 kb on - strand, within Psyr_5000 at 5930.240 kb on - strand, within Psyr_5000 at 5930.309 kb on + strand at 5930.309 kb on + strand at 5930.309 kb on + strand at 5930.377 kb on + strand at 5930.377 kb on + strand at 5930.377 kb on + strand at 5930.377 kb on + strand at 5930.378 kb on - strand at 5930.378 kb on - strand at 5930.378 kb on - strand at 5930.416 kb on + strand at 5930.416 kb on + strand at 5930.416 kb on + strand at 5930.416 kb on + strand at 5930.416 kb on + strand at 5930.416 kb on + strand at 5930.417 kb on - strand at 5930.417 kb on - strand at 5930.417 kb on - strand at 5930.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 5,927,952 + +0.7 5,927,953 - +0.1 5,927,955 + -0.1 5,927,955 + -1.7 5,927,955 + +0.3 5,927,955 + -0.5 5,927,956 - -0.3 5,927,956 - -1.2 5,927,956 - -0.4 5,927,956 - +0.4 5,927,956 - +0.4 5,927,956 - -0.6 5,927,956 - -2.1 5,927,977 + -0.9 5,927,990 + -0.4 5,927,991 - +1.1 5,928,062 - +2.6 5,928,062 - -0.5 5,928,084 + +0.4 5,928,085 - +2.0 5,928,091 - +0.5 5,928,095 - +0.6 5,928,099 - +0.2 5,928,103 + +0.3 5,928,161 + +1.0 5,928,232 + -1.6 5,928,233 - -0.7 5,928,277 + Psyr_4998 0.15 -0.0 5,928,298 + Psyr_4998 0.18 +0.1 5,928,313 + Psyr_4998 0.20 +1.2 5,928,316 - Psyr_4998 0.21 +0.3 5,928,316 - Psyr_4998 0.21 -0.2 5,928,398 + Psyr_4998 0.32 -0.0 5,928,398 + Psyr_4998 0.32 -0.4 5,928,411 + Psyr_4998 0.34 -0.1 5,928,412 - Psyr_4998 0.34 +0.3 5,928,412 - Psyr_4998 0.34 +0.9 5,928,416 + Psyr_4998 0.35 -0.0 5,928,417 - Psyr_4998 0.35 +0.5 5,928,510 + Psyr_4998 0.48 -0.5 5,928,511 - Psyr_4998 0.48 +1.0 5,928,557 + Psyr_4998 0.55 +0.6 5,928,558 - Psyr_4998 0.55 +0.0 5,928,567 + Psyr_4998 0.56 -2.0 5,928,567 + Psyr_4998 0.56 +0.1 5,928,568 - Psyr_4998 0.56 +0.8 5,928,568 - Psyr_4998 0.56 +0.3 5,928,570 - Psyr_4998 0.57 -0.1 5,928,570 - Psyr_4998 0.57 -1.3 5,928,583 - Psyr_4998 0.58 -1.1 5,928,661 + Psyr_4998 0.69 +1.2 5,928,661 + Psyr_4998 0.69 +0.7 5,928,662 - Psyr_4998 0.69 +0.2 5,928,683 + Psyr_4998 0.72 +0.8 5,928,787 - Psyr_4998 0.87 +0.4 5,928,787 - Psyr_4998 0.87 +0.6 5,928,787 - Psyr_4998 0.87 -2.3 5,928,798 + Psyr_4998 0.89 +1.9 5,928,862 + +0.3 5,928,862 + +0.6 5,928,983 + -0.5 5,928,983 + +0.0 5,928,984 - +2.2 5,928,984 - +0.5 5,928,984 - +0.3 5,928,984 - -1.3 5,928,994 - +1.3 5,929,026 + Psyr_4999 0.15 +1.3 5,929,063 + Psyr_4999 0.22 -0.3 5,929,063 + Psyr_4999 0.22 +1.0 5,929,064 - Psyr_4999 0.22 +0.3 5,929,064 - Psyr_4999 0.22 +2.2 5,929,104 + Psyr_4999 0.29 +1.0 5,929,104 + Psyr_4999 0.29 -2.1 5,929,105 - Psyr_4999 0.30 -0.0 5,929,159 + Psyr_4999 0.39 -1.0 5,929,305 + Psyr_4999 0.66 +0.9 5,929,305 + Psyr_4999 0.66 +1.7 5,929,305 + Psyr_4999 0.66 -1.1 5,929,305 + Psyr_4999 0.66 +1.3 5,929,305 + Psyr_4999 0.66 +0.8 5,929,306 - Psyr_4999 0.66 +0.0 5,929,391 + Psyr_4999 0.81 +0.6 5,929,491 + +0.4 5,929,711 - +0.7 5,929,712 + -0.1 5,929,712 + -0.1 5,929,712 + -0.6 5,929,712 + +0.3 5,929,712 + +1.5 5,929,713 - -0.6 5,929,713 - +0.6 5,929,754 + -0.6 5,929,754 + +3.6 5,929,757 + +0.5 5,929,757 + +0.6 5,929,777 + +0.9 5,929,777 + -0.2 5,929,777 + -1.0 5,929,777 + +0.4 5,929,778 - -0.7 5,929,778 - -0.7 5,929,778 - +0.1 5,929,778 - -0.7 5,929,778 - -0.4 5,929,778 - -1.1 5,929,794 + +1.3 5,929,863 + +0.6 5,929,863 + -0.3 5,929,863 + -0.3 5,929,863 + +0.6 5,929,863 + -0.9 5,929,864 - -0.3 5,929,864 - -0.2 5,929,948 + Psyr_5000 0.18 +2.3 5,929,948 + Psyr_5000 0.18 +0.6 5,929,948 + Psyr_5000 0.18 +2.8 5,929,948 + Psyr_5000 0.18 +0.5 5,929,948 + Psyr_5000 0.18 +1.0 5,929,948 + Psyr_5000 0.18 +1.5 5,929,948 + Psyr_5000 0.18 +1.5 5,929,948 + Psyr_5000 0.18 +0.7 5,929,948 + Psyr_5000 0.18 -1.6 5,929,948 + Psyr_5000 0.18 +0.4 5,929,948 + Psyr_5000 0.18 +0.2 5,929,949 - Psyr_5000 0.18 +0.3 5,929,949 - Psyr_5000 0.18 -0.0 5,929,949 - Psyr_5000 0.18 +0.4 5,929,949 - Psyr_5000 0.18 +0.8 5,929,949 - Psyr_5000 0.18 +1.3 5,929,949 - Psyr_5000 0.18 -0.6 5,929,949 - Psyr_5000 0.18 +0.0 5,929,949 - Psyr_5000 0.18 +0.7 5,929,949 - Psyr_5000 0.18 +0.1 5,929,949 - Psyr_5000 0.18 +0.2 5,930,010 + Psyr_5000 0.33 +0.4 5,930,010 + Psyr_5000 0.33 -1.0 5,930,011 - Psyr_5000 0.33 +0.0 5,930,011 - Psyr_5000 0.33 +1.0 5,930,011 - Psyr_5000 0.33 +0.2 5,930,060 - Psyr_5000 0.44 +0.7 5,930,060 - Psyr_5000 0.44 +0.3 5,930,060 - Psyr_5000 0.44 -0.3 5,930,133 - Psyr_5000 0.61 +1.6 5,930,208 + Psyr_5000 0.79 -0.5 5,930,208 + Psyr_5000 0.79 +0.4 5,930,209 - Psyr_5000 0.79 -0.1 5,930,209 - Psyr_5000 0.79 -0.5 5,930,212 + Psyr_5000 0.80 +1.0 5,930,212 + Psyr_5000 0.80 -0.2 5,930,212 + Psyr_5000 0.80 +0.9 5,930,212 + Psyr_5000 0.80 -1.3 5,930,212 + Psyr_5000 0.80 +0.3 5,930,213 - Psyr_5000 0.80 -0.2 5,930,213 - Psyr_5000 0.80 +1.6 5,930,213 - Psyr_5000 0.80 +1.6 5,930,213 - Psyr_5000 0.80 -0.1 5,930,213 - Psyr_5000 0.80 -0.9 5,930,213 - Psyr_5000 0.80 +1.3 5,930,230 + Psyr_5000 0.84 +0.7 5,930,230 + Psyr_5000 0.84 +0.7 5,930,230 + Psyr_5000 0.84 -0.4 5,930,230 + Psyr_5000 0.84 +1.5 5,930,230 + Psyr_5000 0.84 -0.6 5,930,230 + Psyr_5000 0.84 -0.8 5,930,230 + Psyr_5000 0.84 -0.8 5,930,230 + Psyr_5000 0.84 +0.0 5,930,231 - Psyr_5000 0.84 -0.6 5,930,231 - Psyr_5000 0.84 +1.4 5,930,236 + Psyr_5000 0.85 -0.4 5,930,236 + Psyr_5000 0.85 -0.3 5,930,236 + Psyr_5000 0.85 -1.2 5,930,236 + Psyr_5000 0.85 -0.4 5,930,236 + Psyr_5000 0.85 +0.4 5,930,237 - Psyr_5000 0.86 -0.6 5,930,237 - Psyr_5000 0.86 -0.2 5,930,239 + Psyr_5000 0.86 +0.8 5,930,239 + Psyr_5000 0.86 -1.6 5,930,239 + Psyr_5000 0.86 -0.6 5,930,240 - Psyr_5000 0.86 +0.7 5,930,240 - Psyr_5000 0.86 -0.7 5,930,240 - Psyr_5000 0.86 +0.0 5,930,240 - Psyr_5000 0.86 +0.6 5,930,240 - Psyr_5000 0.86 +0.5 5,930,309 + +0.5 5,930,309 + -0.3 5,930,309 + +1.4 5,930,377 + +1.9 5,930,377 + +0.1 5,930,377 + +0.2 5,930,377 + +1.9 5,930,378 - -0.4 5,930,378 - -0.4 5,930,378 - +0.1 5,930,416 + +0.3 5,930,416 + +1.0 5,930,416 + -0.6 5,930,416 + +0.0 5,930,416 + -0.2 5,930,416 + +0.0 5,930,417 - +1.3 5,930,417 - +1.5 5,930,417 - +0.3 5,930,417 - +3.3
Or see this region's nucleotide sequence