Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4672 and Psyr_4673 overlap by 4 nucleotides Psyr_4673 and Psyr_4674 overlap by 8 nucleotides Psyr_4674 and Psyr_4675 overlap by 38 nucleotides
Psyr_4672: Psyr_4672 - Coenzyme PQQ synthesis C, at 5,538,337 to 5,539,092
_4672
Psyr_4673: Psyr_4673 - Coenzyme PQQ synthesis D, at 5,539,089 to 5,539,373
_4673
Psyr_4674: Psyr_4674 - Radical SAM, at 5,539,366 to 5,540,535
_4674
Psyr_4675: Psyr_4675 - Peptidase S9, prolyl oligopeptidase active site region, at 5,540,498 to 5,542,324
_4675
Position (kb) 5539
5540
5541 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5538.400 kb on + strand at 5538.400 kb on + strand at 5538.400 kb on + strand at 5538.401 kb on - strand at 5538.401 kb on - strand at 5538.401 kb on - strand at 5538.401 kb on - strand at 5538.403 kb on + strand at 5538.403 kb on + strand at 5538.403 kb on + strand at 5538.403 kb on + strand at 5538.403 kb on + strand at 5538.403 kb on + strand at 5538.404 kb on - strand at 5538.404 kb on - strand at 5538.412 kb on + strand at 5538.412 kb on + strand at 5538.412 kb on + strand at 5538.412 kb on + strand at 5538.412 kb on + strand at 5538.412 kb on + strand at 5538.412 kb on + strand at 5538.412 kb on + strand at 5538.412 kb on + strand at 5538.412 kb on + strand at 5538.412 kb on + strand at 5538.413 kb on - strand, within Psyr_4672 at 5538.413 kb on - strand, within Psyr_4672 at 5538.413 kb on - strand, within Psyr_4672 at 5538.413 kb on - strand, within Psyr_4672 at 5538.413 kb on - strand, within Psyr_4672 at 5538.413 kb on - strand, within Psyr_4672 at 5538.413 kb on - strand, within Psyr_4672 at 5538.436 kb on + strand, within Psyr_4672 at 5538.436 kb on + strand, within Psyr_4672 at 5538.436 kb on + strand, within Psyr_4672 at 5538.436 kb on + strand, within Psyr_4672 at 5538.437 kb on - strand, within Psyr_4672 at 5538.437 kb on - strand, within Psyr_4672 at 5538.437 kb on - strand, within Psyr_4672 at 5538.490 kb on + strand, within Psyr_4672 at 5538.490 kb on + strand, within Psyr_4672 at 5538.490 kb on + strand, within Psyr_4672 at 5538.490 kb on + strand, within Psyr_4672 at 5538.491 kb on - strand, within Psyr_4672 at 5538.491 kb on - strand, within Psyr_4672 at 5538.491 kb on - strand, within Psyr_4672 at 5538.491 kb on - strand, within Psyr_4672 at 5538.491 kb on - strand, within Psyr_4672 at 5538.493 kb on + strand, within Psyr_4672 at 5538.493 kb on + strand, within Psyr_4672 at 5538.494 kb on - strand, within Psyr_4672 at 5538.719 kb on - strand, within Psyr_4672 at 5538.853 kb on + strand, within Psyr_4672 at 5538.853 kb on + strand, within Psyr_4672 at 5538.859 kb on + strand, within Psyr_4672 at 5538.860 kb on - strand, within Psyr_4672 at 5538.860 kb on - strand, within Psyr_4672 at 5538.860 kb on - strand, within Psyr_4672 at 5538.860 kb on - strand, within Psyr_4672 at 5538.860 kb on - strand, within Psyr_4672 at 5538.896 kb on + strand, within Psyr_4672 at 5538.896 kb on + strand, within Psyr_4672 at 5538.896 kb on + strand, within Psyr_4672 at 5538.896 kb on + strand, within Psyr_4672 at 5538.896 kb on + strand, within Psyr_4672 at 5538.896 kb on + strand, within Psyr_4672 at 5538.896 kb on + strand, within Psyr_4672 at 5538.897 kb on - strand, within Psyr_4672 at 5538.897 kb on - strand, within Psyr_4672 at 5538.897 kb on - strand, within Psyr_4672 at 5538.897 kb on - strand, within Psyr_4672 at 5538.920 kb on + strand, within Psyr_4672 at 5538.921 kb on - strand, within Psyr_4672 at 5538.928 kb on + strand, within Psyr_4672 at 5538.928 kb on + strand, within Psyr_4672 at 5538.928 kb on + strand, within Psyr_4672 at 5538.928 kb on + strand, within Psyr_4672 at 5538.928 kb on + strand, within Psyr_4672 at 5538.928 kb on + strand, within Psyr_4672 at 5538.928 kb on + strand, within Psyr_4672 at 5538.929 kb on - strand, within Psyr_4672 at 5538.929 kb on - strand, within Psyr_4672 at 5538.929 kb on - strand, within Psyr_4672 at 5538.929 kb on - strand, within Psyr_4672 at 5538.929 kb on - strand, within Psyr_4672 at 5538.929 kb on - strand, within Psyr_4672 at 5538.929 kb on - strand, within Psyr_4672 at 5538.929 kb on - strand, within Psyr_4672 at 5538.933 kb on + strand, within Psyr_4672 at 5538.933 kb on + strand, within Psyr_4672 at 5538.933 kb on + strand, within Psyr_4672 at 5538.933 kb on + strand, within Psyr_4672 at 5538.933 kb on + strand, within Psyr_4672 at 5538.933 kb on + strand, within Psyr_4672 at 5538.934 kb on - strand, within Psyr_4672 at 5538.934 kb on - strand, within Psyr_4672 at 5538.934 kb on - strand, within Psyr_4672 at 5538.934 kb on - strand, within Psyr_4672 at 5538.934 kb on - strand, within Psyr_4672 at 5538.934 kb on - strand, within Psyr_4672 at 5538.934 kb on - strand, within Psyr_4672 at 5538.934 kb on - strand, within Psyr_4672 at 5538.934 kb on - strand, within Psyr_4672 at 5538.934 kb on - strand, within Psyr_4672 at 5538.934 kb on - strand, within Psyr_4672 at 5538.934 kb on - strand, within Psyr_4672 at 5538.937 kb on + strand, within Psyr_4672 at 5538.937 kb on + strand, within Psyr_4672 at 5538.937 kb on + strand, within Psyr_4672 at 5538.937 kb on + strand, within Psyr_4672 at 5538.937 kb on + strand, within Psyr_4672 at 5538.938 kb on - strand, within Psyr_4672 at 5538.938 kb on - strand, within Psyr_4672 at 5538.938 kb on - strand, within Psyr_4672 at 5538.955 kb on - strand, within Psyr_4672 at 5538.955 kb on - strand, within Psyr_4672 at 5538.955 kb on - strand, within Psyr_4672 at 5538.955 kb on - strand, within Psyr_4672 at 5538.955 kb on - strand, within Psyr_4672 at 5539.021 kb on + strand at 5539.074 kb on + strand at 5539.088 kb on + strand at 5539.089 kb on - strand at 5539.089 kb on - strand at 5539.089 kb on - strand at 5539.089 kb on - strand at 5539.185 kb on + strand, within Psyr_4673 at 5539.186 kb on - strand, within Psyr_4673 at 5539.376 kb on + strand at 5539.376 kb on + strand at 5539.376 kb on + strand at 5539.376 kb on + strand at 5539.376 kb on + strand at 5539.376 kb on + strand at 5539.377 kb on - strand at 5539.377 kb on - strand at 5539.377 kb on - strand at 5539.377 kb on - strand at 5539.377 kb on - strand at 5539.377 kb on - strand at 5539.377 kb on - strand at 5539.377 kb on - strand at 5539.377 kb on - strand at 5539.377 kb on - strand at 5539.377 kb on - strand at 5539.377 kb on - strand at 5539.399 kb on + strand at 5539.400 kb on - strand at 5539.400 kb on - strand at 5539.456 kb on + strand at 5539.456 kb on + strand at 5539.457 kb on - strand at 5539.457 kb on - strand at 5539.457 kb on - strand at 5539.481 kb on - strand at 5539.660 kb on + strand, within Psyr_4674 at 5539.660 kb on + strand, within Psyr_4674 at 5539.660 kb on + strand, within Psyr_4674 at 5539.966 kb on + strand, within Psyr_4674 at 5539.966 kb on + strand, within Psyr_4674 at 5539.967 kb on - strand, within Psyr_4674 at 5539.967 kb on - strand, within Psyr_4674 at 5539.967 kb on - strand, within Psyr_4674 at 5540.107 kb on + strand, within Psyr_4674 at 5540.107 kb on + strand, within Psyr_4674 at 5540.108 kb on - strand, within Psyr_4674 at 5540.108 kb on - strand, within Psyr_4674 at 5540.111 kb on - strand, within Psyr_4674 at 5540.151 kb on + strand, within Psyr_4674 at 5540.152 kb on - strand, within Psyr_4674 at 5540.261 kb on - strand, within Psyr_4674 at 5540.434 kb on - strand at 5540.434 kb on - strand at 5540.704 kb on - strand, within Psyr_4675 at 5540.717 kb on + strand, within Psyr_4675 at 5540.717 kb on + strand, within Psyr_4675 at 5540.717 kb on + strand, within Psyr_4675 at 5540.717 kb on + strand, within Psyr_4675 at 5540.717 kb on + strand, within Psyr_4675 at 5540.718 kb on - strand, within Psyr_4675 at 5540.718 kb on - strand, within Psyr_4675 at 5540.718 kb on - strand, within Psyr_4675 at 5540.718 kb on - strand, within Psyr_4675 at 5540.718 kb on - strand, within Psyr_4675 at 5540.718 kb on - strand, within Psyr_4675 at 5540.723 kb on + strand, within Psyr_4675 at 5540.792 kb on + strand, within Psyr_4675 at 5540.976 kb on - strand, within Psyr_4675 at 5541.176 kb on + strand, within Psyr_4675 at 5541.176 kb on + strand, within Psyr_4675 at 5541.177 kb on - strand, within Psyr_4675 at 5541.446 kb on + strand, within Psyr_4675 at 5541.447 kb on - strand, within Psyr_4675 at 5541.447 kb on - strand, within Psyr_4675
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 5,538,400 + +0.3 5,538,400 + +0.7 5,538,400 + +0.4 5,538,401 - +0.9 5,538,401 - +1.0 5,538,401 - -0.5 5,538,401 - -0.5 5,538,403 + -0.0 5,538,403 + -0.7 5,538,403 + -0.1 5,538,403 + +0.4 5,538,403 + -0.7 5,538,403 + -0.5 5,538,404 - +1.0 5,538,404 - +2.3 5,538,412 + -1.6 5,538,412 + +0.5 5,538,412 + +0.7 5,538,412 + -0.9 5,538,412 + -0.6 5,538,412 + -0.4 5,538,412 + +1.2 5,538,412 + +0.1 5,538,412 + -0.4 5,538,412 + +0.3 5,538,412 + -0.3 5,538,413 - Psyr_4672 0.10 -0.1 5,538,413 - Psyr_4672 0.10 +1.1 5,538,413 - Psyr_4672 0.10 +0.2 5,538,413 - Psyr_4672 0.10 -0.4 5,538,413 - Psyr_4672 0.10 +0.4 5,538,413 - Psyr_4672 0.10 -0.4 5,538,413 - Psyr_4672 0.10 -0.4 5,538,436 + Psyr_4672 0.13 +0.3 5,538,436 + Psyr_4672 0.13 +0.2 5,538,436 + Psyr_4672 0.13 -0.3 5,538,436 + Psyr_4672 0.13 +0.3 5,538,437 - Psyr_4672 0.13 -0.8 5,538,437 - Psyr_4672 0.13 +2.0 5,538,437 - Psyr_4672 0.13 +1.3 5,538,490 + Psyr_4672 0.20 -2.7 5,538,490 + Psyr_4672 0.20 -1.0 5,538,490 + Psyr_4672 0.20 -0.9 5,538,490 + Psyr_4672 0.20 +0.7 5,538,491 - Psyr_4672 0.20 +0.9 5,538,491 - Psyr_4672 0.20 -0.1 5,538,491 - Psyr_4672 0.20 +0.2 5,538,491 - Psyr_4672 0.20 -0.6 5,538,491 - Psyr_4672 0.20 -0.7 5,538,493 + Psyr_4672 0.21 -0.8 5,538,493 + Psyr_4672 0.21 +0.6 5,538,494 - Psyr_4672 0.21 -0.5 5,538,719 - Psyr_4672 0.51 +0.2 5,538,853 + Psyr_4672 0.68 -1.0 5,538,853 + Psyr_4672 0.68 +1.9 5,538,859 + Psyr_4672 0.69 +0.9 5,538,860 - Psyr_4672 0.69 +0.2 5,538,860 - Psyr_4672 0.69 -0.3 5,538,860 - Psyr_4672 0.69 +0.1 5,538,860 - Psyr_4672 0.69 -0.3 5,538,860 - Psyr_4672 0.69 +1.1 5,538,896 + Psyr_4672 0.74 -1.1 5,538,896 + Psyr_4672 0.74 -0.5 5,538,896 + Psyr_4672 0.74 +1.8 5,538,896 + Psyr_4672 0.74 -0.8 5,538,896 + Psyr_4672 0.74 +0.4 5,538,896 + Psyr_4672 0.74 -0.7 5,538,896 + Psyr_4672 0.74 -0.5 5,538,897 - Psyr_4672 0.74 +1.7 5,538,897 - Psyr_4672 0.74 +0.2 5,538,897 - Psyr_4672 0.74 +0.3 5,538,897 - Psyr_4672 0.74 +0.9 5,538,920 + Psyr_4672 0.77 -0.7 5,538,921 - Psyr_4672 0.77 +0.3 5,538,928 + Psyr_4672 0.78 +0.3 5,538,928 + Psyr_4672 0.78 +0.8 5,538,928 + Psyr_4672 0.78 -0.4 5,538,928 + Psyr_4672 0.78 +1.0 5,538,928 + Psyr_4672 0.78 +0.1 5,538,928 + Psyr_4672 0.78 +0.4 5,538,928 + Psyr_4672 0.78 -0.2 5,538,929 - Psyr_4672 0.78 +1.2 5,538,929 - Psyr_4672 0.78 -1.4 5,538,929 - Psyr_4672 0.78 -0.3 5,538,929 - Psyr_4672 0.78 -1.1 5,538,929 - Psyr_4672 0.78 +0.1 5,538,929 - Psyr_4672 0.78 -1.3 5,538,929 - Psyr_4672 0.78 -0.7 5,538,929 - Psyr_4672 0.78 +1.9 5,538,933 + Psyr_4672 0.79 -0.4 5,538,933 + Psyr_4672 0.79 +0.8 5,538,933 + Psyr_4672 0.79 +0.3 5,538,933 + Psyr_4672 0.79 -0.5 5,538,933 + Psyr_4672 0.79 +0.6 5,538,933 + Psyr_4672 0.79 -0.9 5,538,934 - Psyr_4672 0.79 +0.3 5,538,934 - Psyr_4672 0.79 -0.3 5,538,934 - Psyr_4672 0.79 -1.1 5,538,934 - Psyr_4672 0.79 -0.1 5,538,934 - Psyr_4672 0.79 -0.3 5,538,934 - Psyr_4672 0.79 -1.0 5,538,934 - Psyr_4672 0.79 +0.8 5,538,934 - Psyr_4672 0.79 +1.5 5,538,934 - Psyr_4672 0.79 -0.1 5,538,934 - Psyr_4672 0.79 +0.8 5,538,934 - Psyr_4672 0.79 -0.4 5,538,934 - Psyr_4672 0.79 -0.5 5,538,937 + Psyr_4672 0.79 -1.1 5,538,937 + Psyr_4672 0.79 +0.8 5,538,937 + Psyr_4672 0.79 -0.3 5,538,937 + Psyr_4672 0.79 -0.3 5,538,937 + Psyr_4672 0.79 +0.3 5,538,938 - Psyr_4672 0.79 +0.4 5,538,938 - Psyr_4672 0.79 -0.9 5,538,938 - Psyr_4672 0.79 +0.0 5,538,955 - Psyr_4672 0.82 +2.5 5,538,955 - Psyr_4672 0.82 +0.1 5,538,955 - Psyr_4672 0.82 +0.5 5,538,955 - Psyr_4672 0.82 -0.5 5,538,955 - Psyr_4672 0.82 -0.2 5,539,021 + +1.2 5,539,074 + -0.2 5,539,088 + +0.4 5,539,089 - +1.0 5,539,089 - +1.9 5,539,089 - +0.9 5,539,089 - +1.1 5,539,185 + Psyr_4673 0.34 -0.3 5,539,186 - Psyr_4673 0.34 -0.0 5,539,376 + +1.9 5,539,376 + -1.6 5,539,376 + -0.5 5,539,376 + -0.3 5,539,376 + +0.0 5,539,376 + -0.5 5,539,377 - +0.4 5,539,377 - +0.6 5,539,377 - +0.4 5,539,377 - +1.9 5,539,377 - -0.3 5,539,377 - +0.6 5,539,377 - +1.7 5,539,377 - +0.2 5,539,377 - +0.2 5,539,377 - +0.0 5,539,377 - -0.1 5,539,377 - +2.8 5,539,399 + -0.7 5,539,400 - -0.7 5,539,400 - -0.3 5,539,456 + -0.7 5,539,456 + +0.7 5,539,457 - +0.0 5,539,457 - +0.5 5,539,457 - +2.3 5,539,481 - +0.7 5,539,660 + Psyr_4674 0.25 -0.2 5,539,660 + Psyr_4674 0.25 -0.5 5,539,660 + Psyr_4674 0.25 -0.1 5,539,966 + Psyr_4674 0.51 +0.6 5,539,966 + Psyr_4674 0.51 +0.9 5,539,967 - Psyr_4674 0.51 +0.7 5,539,967 - Psyr_4674 0.51 +0.2 5,539,967 - Psyr_4674 0.51 +0.5 5,540,107 + Psyr_4674 0.63 +0.7 5,540,107 + Psyr_4674 0.63 +0.3 5,540,108 - Psyr_4674 0.63 +1.4 5,540,108 - Psyr_4674 0.63 +1.3 5,540,111 - Psyr_4674 0.64 -1.7 5,540,151 + Psyr_4674 0.67 -0.1 5,540,152 - Psyr_4674 0.67 -0.6 5,540,261 - Psyr_4674 0.76 +0.9 5,540,434 - -0.1 5,540,434 - -0.3 5,540,704 - Psyr_4675 0.11 +1.2 5,540,717 + Psyr_4675 0.12 +0.5 5,540,717 + Psyr_4675 0.12 +0.4 5,540,717 + Psyr_4675 0.12 -0.1 5,540,717 + Psyr_4675 0.12 +0.9 5,540,717 + Psyr_4675 0.12 -2.5 5,540,718 - Psyr_4675 0.12 +1.0 5,540,718 - Psyr_4675 0.12 +0.2 5,540,718 - Psyr_4675 0.12 +0.8 5,540,718 - Psyr_4675 0.12 +1.1 5,540,718 - Psyr_4675 0.12 +0.3 5,540,718 - Psyr_4675 0.12 +0.4 5,540,723 + Psyr_4675 0.12 -0.0 5,540,792 + Psyr_4675 0.16 -1.0 5,540,976 - Psyr_4675 0.26 -2.5 5,541,176 + Psyr_4675 0.37 -0.3 5,541,176 + Psyr_4675 0.37 +0.7 5,541,177 - Psyr_4675 0.37 -0.7 5,541,446 + Psyr_4675 0.52 -1.4 5,541,447 - Psyr_4675 0.52 +1.7 5,541,447 - Psyr_4675 0.52 -1.2
Or see this region's nucleotide sequence