Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3972 and Psyr_3973 are separated by 27 nucleotides Psyr_3973 and Psyr_3974 overlap by 4 nucleotides Psyr_3974 and Psyr_3975 overlap by 4 nucleotides Psyr_3975 and Psyr_3976 are separated by 11 nucleotides
Psyr_3972: Psyr_3972 - ABC transporter, periplasmic substrate-binding protein, at 4,719,943 to 4,721,010
_3972
Psyr_3973: Psyr_3973 - Binding-protein-dependent transport systems inner membrane component, at 4,721,038 to 4,721,853
_3973
Psyr_3974: Psyr_3974 - ABC transporter, at 4,721,850 to 4,722,632
_3974
Psyr_3975: Psyr_3975 - conserved hypothetical protein, at 4,722,629 to 4,723,354
_3975
Psyr_3976: Psyr_3976 - conserved hypothetical protein, at 4,723,366 to 4,723,983
_3976
Position (kb)
4721
4722
4723 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4720.974 kb on + strand at 4720.974 kb on + strand at 4720.974 kb on + strand at 4720.975 kb on - strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4721.008 kb on + strand at 4721.008 kb on + strand at 4721.009 kb on - strand at 4721.009 kb on - strand at 4721.022 kb on + strand at 4721.023 kb on - strand at 4721.081 kb on + strand at 4721.120 kb on - strand, within Psyr_3973 at 4721.120 kb on - strand, within Psyr_3973 at 4721.243 kb on - strand, within Psyr_3973 at 4721.729 kb on - strand, within Psyr_3973 at 4721.755 kb on + strand, within Psyr_3973 at 4721.755 kb on + strand, within Psyr_3973 at 4721.755 kb on + strand, within Psyr_3973 at 4721.755 kb on + strand, within Psyr_3973 at 4721.755 kb on + strand, within Psyr_3973 at 4721.756 kb on - strand, within Psyr_3973 at 4721.756 kb on - strand, within Psyr_3973 at 4721.756 kb on - strand, within Psyr_3973 at 4721.887 kb on - strand at 4722.258 kb on + strand, within Psyr_3974 at 4722.499 kb on + strand, within Psyr_3974 at 4722.500 kb on - strand, within Psyr_3974 at 4722.500 kb on - strand, within Psyr_3974 at 4722.546 kb on + strand, within Psyr_3974 at 4722.546 kb on + strand, within Psyr_3974 at 4722.547 kb on - strand, within Psyr_3974 at 4722.547 kb on - strand, within Psyr_3974 at 4722.547 kb on - strand, within Psyr_3974 at 4722.547 kb on - strand, within Psyr_3974 at 4722.705 kb on + strand, within Psyr_3975 at 4722.706 kb on - strand, within Psyr_3975 at 4722.860 kb on + strand, within Psyr_3975 at 4722.861 kb on - strand, within Psyr_3975 at 4722.902 kb on - strand, within Psyr_3975 at 4722.902 kb on - strand, within Psyr_3975 at 4722.963 kb on - strand, within Psyr_3975 at 4723.154 kb on + strand, within Psyr_3975 at 4723.154 kb on + strand, within Psyr_3975 at 4723.154 kb on + strand, within Psyr_3975 at 4723.154 kb on + strand, within Psyr_3975 at 4723.155 kb on - strand, within Psyr_3975 at 4723.155 kb on - strand, within Psyr_3975 at 4723.155 kb on - strand, within Psyr_3975 at 4723.155 kb on - strand, within Psyr_3975 at 4723.155 kb on - strand, within Psyr_3975 at 4723.167 kb on - strand, within Psyr_3975 at 4723.233 kb on - strand, within Psyr_3975 at 4723.565 kb on - strand, within Psyr_3976 at 4723.565 kb on - strand, within Psyr_3976
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 4,720,974 + -1.2 4,720,974 + -0.7 4,720,974 + +0.7 4,720,975 - +1.0 4,720,978 + -3.2 4,720,978 + +0.6 4,720,978 + -0.0 4,720,978 + -0.2 4,720,978 + +0.7 4,720,978 + +0.4 4,720,978 + +0.6 4,720,978 + +0.7 4,720,978 + -0.0 4,720,978 + -0.6 4,720,979 - +1.3 4,720,979 - -0.6 4,720,979 - +0.2 4,720,979 - +0.2 4,720,979 - +0.4 4,720,979 - +1.1 4,720,979 - +0.3 4,720,979 - +0.2 4,720,979 - +0.7 4,721,008 + +0.0 4,721,008 + -0.4 4,721,009 - +0.4 4,721,009 - -0.3 4,721,022 + +0.2 4,721,023 - +0.2 4,721,081 + -0.3 4,721,120 - Psyr_3973 0.10 -0.4 4,721,120 - Psyr_3973 0.10 -0.6 4,721,243 - Psyr_3973 0.25 +0.5 4,721,729 - Psyr_3973 0.85 -0.1 4,721,755 + Psyr_3973 0.88 -1.6 4,721,755 + Psyr_3973 0.88 -0.2 4,721,755 + Psyr_3973 0.88 +0.5 4,721,755 + Psyr_3973 0.88 -0.0 4,721,755 + Psyr_3973 0.88 -0.9 4,721,756 - Psyr_3973 0.88 +0.7 4,721,756 - Psyr_3973 0.88 +0.2 4,721,756 - Psyr_3973 0.88 -0.6 4,721,887 - +0.4 4,722,258 + Psyr_3974 0.52 +1.6 4,722,499 + Psyr_3974 0.83 -0.6 4,722,500 - Psyr_3974 0.83 +0.1 4,722,500 - Psyr_3974 0.83 +1.2 4,722,546 + Psyr_3974 0.89 +0.3 4,722,546 + Psyr_3974 0.89 -0.3 4,722,547 - Psyr_3974 0.89 -1.7 4,722,547 - Psyr_3974 0.89 +0.4 4,722,547 - Psyr_3974 0.89 -0.3 4,722,547 - Psyr_3974 0.89 +1.8 4,722,705 + Psyr_3975 0.10 -0.7 4,722,706 - Psyr_3975 0.11 +0.9 4,722,860 + Psyr_3975 0.32 +1.0 4,722,861 - Psyr_3975 0.32 -0.3 4,722,902 - Psyr_3975 0.38 -0.3 4,722,902 - Psyr_3975 0.38 -0.5 4,722,963 - Psyr_3975 0.46 -0.4 4,723,154 + Psyr_3975 0.72 +0.1 4,723,154 + Psyr_3975 0.72 -0.8 4,723,154 + Psyr_3975 0.72 -0.1 4,723,154 + Psyr_3975 0.72 +1.3 4,723,155 - Psyr_3975 0.72 +2.9 4,723,155 - Psyr_3975 0.72 -1.1 4,723,155 - Psyr_3975 0.72 +0.1 4,723,155 - Psyr_3975 0.72 -0.3 4,723,155 - Psyr_3975 0.72 +0.3 4,723,167 - Psyr_3975 0.74 +1.2 4,723,233 - Psyr_3975 0.83 +0.0 4,723,565 - Psyr_3976 0.32 -0.4 4,723,565 - Psyr_3976 0.32 -0.3
Or see this region's nucleotide sequence