Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3544 and Psyr_3545 are separated by 101 nucleotides Psyr_3545 and Psyr_3546 are separated by 138 nucleotides
Psyr_3544: Psyr_3544 - putative integral membrane protein, at 4,221,692 to 4,222,666
_3544
Psyr_3545: Psyr_3545 - Short-chain dehydrogenase/reductase SDR, at 4,222,768 to 4,223,511
_3545
Psyr_3546: Psyr_3546 - transcriptional regulator, AraC family, at 4,223,650 to 4,224,558
_3546
Position (kb)
4222
4223
4224 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 4221.827 kb on - strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.832 kb on + strand, within Psyr_3544 at 4221.832 kb on + strand, within Psyr_3544 at 4221.832 kb on + strand, within Psyr_3544 at 4221.833 kb on - strand, within Psyr_3544 at 4221.835 kb on - strand, within Psyr_3544 at 4221.989 kb on - strand, within Psyr_3544 at 4222.007 kb on - strand, within Psyr_3544 at 4222.393 kb on - strand, within Psyr_3544 at 4222.412 kb on - strand, within Psyr_3544 at 4222.412 kb on - strand, within Psyr_3544 at 4222.647 kb on + strand at 4222.706 kb on + strand at 4222.738 kb on + strand at 4222.739 kb on - strand at 4222.746 kb on + strand at 4222.746 kb on + strand at 4222.747 kb on - strand at 4222.747 kb on - strand at 4222.978 kb on + strand, within Psyr_3545 at 4223.007 kb on + strand, within Psyr_3545 at 4223.007 kb on + strand, within Psyr_3545 at 4223.007 kb on + strand, within Psyr_3545 at 4223.008 kb on - strand, within Psyr_3545 at 4223.009 kb on + strand, within Psyr_3545 at 4223.010 kb on - strand, within Psyr_3545 at 4223.049 kb on - strand, within Psyr_3545 at 4223.417 kb on + strand, within Psyr_3545 at 4223.422 kb on - strand, within Psyr_3545 at 4223.422 kb on - strand, within Psyr_3545 at 4223.497 kb on + strand at 4223.498 kb on - strand at 4223.720 kb on + strand at 4223.720 kb on + strand at 4223.721 kb on - strand at 4223.721 kb on - strand at 4223.721 kb on - strand at 4223.760 kb on + strand, within Psyr_3546 at 4223.760 kb on + strand, within Psyr_3546 at 4223.760 kb on + strand, within Psyr_3546 at 4223.760 kb on + strand, within Psyr_3546 at 4223.760 kb on + strand, within Psyr_3546 at 4223.761 kb on - strand, within Psyr_3546 at 4223.761 kb on - strand, within Psyr_3546 at 4223.761 kb on - strand, within Psyr_3546 at 4223.761 kb on - strand, within Psyr_3546 at 4223.761 kb on - strand, within Psyr_3546 at 4223.761 kb on - strand, within Psyr_3546 at 4223.761 kb on - strand, within Psyr_3546 at 4223.761 kb on - strand, within Psyr_3546 at 4223.860 kb on + strand, within Psyr_3546 at 4223.861 kb on - strand, within Psyr_3546 at 4223.927 kb on - strand, within Psyr_3546 at 4224.136 kb on - strand, within Psyr_3546 at 4224.136 kb on - strand, within Psyr_3546 at 4224.350 kb on - strand, within Psyr_3546
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 4,221,827 - Psyr_3544 0.14 -1.2 4,221,828 + Psyr_3544 0.14 +1.2 4,221,828 + Psyr_3544 0.14 +1.0 4,221,828 + Psyr_3544 0.14 -0.2 4,221,828 + Psyr_3544 0.14 +0.3 4,221,828 + Psyr_3544 0.14 -0.4 4,221,828 + Psyr_3544 0.14 +2.1 4,221,828 + Psyr_3544 0.14 -0.4 4,221,828 + Psyr_3544 0.14 +0.1 4,221,828 + Psyr_3544 0.14 -0.7 4,221,828 + Psyr_3544 0.14 +1.5 4,221,829 - Psyr_3544 0.14 +1.0 4,221,829 - Psyr_3544 0.14 -1.0 4,221,829 - Psyr_3544 0.14 +0.6 4,221,829 - Psyr_3544 0.14 -0.6 4,221,829 - Psyr_3544 0.14 -0.3 4,221,829 - Psyr_3544 0.14 -0.4 4,221,829 - Psyr_3544 0.14 +0.7 4,221,832 + Psyr_3544 0.14 +0.1 4,221,832 + Psyr_3544 0.14 +0.0 4,221,832 + Psyr_3544 0.14 +0.1 4,221,833 - Psyr_3544 0.14 -0.1 4,221,835 - Psyr_3544 0.15 +0.2 4,221,989 - Psyr_3544 0.30 +1.9 4,222,007 - Psyr_3544 0.32 +1.0 4,222,393 - Psyr_3544 0.72 +1.0 4,222,412 - Psyr_3544 0.74 -0.1 4,222,412 - Psyr_3544 0.74 +0.3 4,222,647 + +0.8 4,222,706 + +2.0 4,222,738 + -0.4 4,222,739 - +1.5 4,222,746 + -0.1 4,222,746 + +1.1 4,222,747 - +0.4 4,222,747 - +1.0 4,222,978 + Psyr_3545 0.28 -0.8 4,223,007 + Psyr_3545 0.32 -0.2 4,223,007 + Psyr_3545 0.32 +0.1 4,223,007 + Psyr_3545 0.32 +2.9 4,223,008 - Psyr_3545 0.32 +0.4 4,223,009 + Psyr_3545 0.32 +1.8 4,223,010 - Psyr_3545 0.33 +0.8 4,223,049 - Psyr_3545 0.38 -1.7 4,223,417 + Psyr_3545 0.87 +0.8 4,223,422 - Psyr_3545 0.88 -0.4 4,223,422 - Psyr_3545 0.88 +2.4 4,223,497 + -0.2 4,223,498 - +0.6 4,223,720 + +2.6 4,223,720 + -0.0 4,223,721 - -0.2 4,223,721 - +0.6 4,223,721 - -0.6 4,223,760 + Psyr_3546 0.12 +0.2 4,223,760 + Psyr_3546 0.12 -0.3 4,223,760 + Psyr_3546 0.12 -1.1 4,223,760 + Psyr_3546 0.12 -1.5 4,223,760 + Psyr_3546 0.12 +0.9 4,223,761 - Psyr_3546 0.12 +0.6 4,223,761 - Psyr_3546 0.12 -1.3 4,223,761 - Psyr_3546 0.12 -0.1 4,223,761 - Psyr_3546 0.12 +0.7 4,223,761 - Psyr_3546 0.12 +1.6 4,223,761 - Psyr_3546 0.12 -1.1 4,223,761 - Psyr_3546 0.12 +0.1 4,223,761 - Psyr_3546 0.12 -0.4 4,223,860 + Psyr_3546 0.23 -0.4 4,223,861 - Psyr_3546 0.23 +0.3 4,223,927 - Psyr_3546 0.30 +3.9 4,224,136 - Psyr_3546 0.53 -0.4 4,224,136 - Psyr_3546 0.53 -0.7 4,224,350 - Psyr_3546 0.77 -0.3
Or see this region's nucleotide sequence