Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3371 and Psyr_3372 overlap by 4 nucleotides Psyr_3372 and Psyr_3373 are separated by 74 nucleotides Psyr_3373 and Psyr_3374 are separated by 170 nucleotides Psyr_3374 and Psyr_3375 overlap by 22 nucleotides
Psyr_3371: Psyr_3371 - Oxidoreductase, molybdopterin binding protein, at 4,030,704 to 4,031,474
_3371
Psyr_3372: Psyr_3372 - conserved hypothetical protein, at 4,031,471 to 4,032,091
_3372
Psyr_3373: Psyr_3373 - conserved hypothetical protein, at 4,032,166 to 4,032,771
_3373
Psyr_3374: Psyr_3374 - Heavy metal response regulator, at 4,032,942 to 4,033,631
_3374
Psyr_3375: Psyr_3375 - Heavy metal sensor kinase, at 4,033,610 to 4,034,998
_3375
Position (kb)
4032
4033 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4031.176 kb on + strand, within Psyr_3371 at 4031.176 kb on + strand, within Psyr_3371 at 4031.176 kb on + strand, within Psyr_3371 at 4031.176 kb on + strand, within Psyr_3371 at 4031.176 kb on + strand, within Psyr_3371 at 4031.177 kb on - strand, within Psyr_3371 at 4031.177 kb on - strand, within Psyr_3371 at 4031.177 kb on - strand, within Psyr_3371 at 4031.177 kb on - strand, within Psyr_3371 at 4031.228 kb on + strand, within Psyr_3371 at 4031.230 kb on + strand, within Psyr_3371 at 4031.230 kb on + strand, within Psyr_3371 at 4031.230 kb on + strand, within Psyr_3371 at 4031.231 kb on - strand, within Psyr_3371 at 4031.231 kb on - strand, within Psyr_3371 at 4031.231 kb on - strand, within Psyr_3371 at 4031.231 kb on - strand, within Psyr_3371 at 4031.453 kb on + strand at 4031.453 kb on + strand at 4031.459 kb on + strand at 4031.533 kb on + strand, within Psyr_3372 at 4031.534 kb on - strand, within Psyr_3372 at 4031.607 kb on - strand, within Psyr_3372 at 4031.699 kb on + strand, within Psyr_3372 at 4031.724 kb on + strand, within Psyr_3372 at 4031.724 kb on + strand, within Psyr_3372 at 4031.724 kb on + strand, within Psyr_3372 at 4031.724 kb on + strand, within Psyr_3372 at 4031.724 kb on + strand, within Psyr_3372 at 4031.724 kb on + strand, within Psyr_3372 at 4031.725 kb on - strand, within Psyr_3372 at 4031.725 kb on - strand, within Psyr_3372 at 4031.725 kb on - strand, within Psyr_3372 at 4031.725 kb on - strand, within Psyr_3372 at 4031.725 kb on - strand, within Psyr_3372 at 4031.725 kb on - strand, within Psyr_3372 at 4031.871 kb on + strand, within Psyr_3372 at 4031.872 kb on - strand, within Psyr_3372 at 4031.872 kb on - strand, within Psyr_3372 at 4031.872 kb on - strand, within Psyr_3372 at 4031.994 kb on + strand, within Psyr_3372 at 4032.019 kb on + strand, within Psyr_3372 at 4032.019 kb on + strand, within Psyr_3372 at 4032.019 kb on + strand, within Psyr_3372 at 4032.019 kb on + strand, within Psyr_3372 at 4032.019 kb on + strand, within Psyr_3372 at 4032.019 kb on + strand, within Psyr_3372 at 4032.019 kb on + strand, within Psyr_3372 at 4032.020 kb on - strand, within Psyr_3372 at 4032.020 kb on - strand, within Psyr_3372 at 4032.020 kb on - strand, within Psyr_3372 at 4032.020 kb on - strand, within Psyr_3372 at 4032.020 kb on - strand, within Psyr_3372 at 4032.167 kb on + strand at 4032.167 kb on + strand at 4032.167 kb on + strand at 4032.167 kb on + strand at 4032.168 kb on - strand at 4032.168 kb on - strand at 4032.168 kb on - strand at 4032.168 kb on - strand at 4032.238 kb on + strand, within Psyr_3373 at 4032.341 kb on + strand, within Psyr_3373 at 4032.341 kb on + strand, within Psyr_3373 at 4032.341 kb on + strand, within Psyr_3373 at 4032.341 kb on + strand, within Psyr_3373 at 4032.341 kb on + strand, within Psyr_3373 at 4032.342 kb on - strand, within Psyr_3373 at 4032.342 kb on - strand, within Psyr_3373 at 4032.342 kb on - strand, within Psyr_3373 at 4032.342 kb on - strand, within Psyr_3373 at 4032.342 kb on - strand, within Psyr_3373 at 4032.609 kb on + strand, within Psyr_3373 at 4032.750 kb on + strand at 4032.751 kb on - strand at 4032.751 kb on - strand at 4032.751 kb on - strand at 4032.753 kb on + strand at 4032.753 kb on + strand at 4032.775 kb on + strand at 4032.776 kb on - strand at 4032.829 kb on + strand at 4032.829 kb on + strand at 4032.829 kb on + strand at 4032.829 kb on + strand at 4032.830 kb on - strand at 4032.830 kb on - strand at 4032.830 kb on - strand at 4032.888 kb on - strand at 4032.947 kb on + strand at 4032.955 kb on + strand at 4032.956 kb on - strand at 4032.956 kb on - strand at 4033.230 kb on + strand, within Psyr_3374 at 4033.301 kb on - strand, within Psyr_3374 at 4033.442 kb on - strand, within Psyr_3374 at 4033.442 kb on - strand, within Psyr_3374 at 4033.442 kb on - strand, within Psyr_3374 at 4033.442 kb on - strand, within Psyr_3374 at 4033.590 kb on + strand at 4033.590 kb on + strand at 4033.591 kb on - strand at 4033.694 kb on + strand at 4033.695 kb on - strand at 4033.695 kb on - strand at 4033.700 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 4,031,176 + Psyr_3371 0.61 -2.0 4,031,176 + Psyr_3371 0.61 -0.1 4,031,176 + Psyr_3371 0.61 +0.7 4,031,176 + Psyr_3371 0.61 +0.9 4,031,176 + Psyr_3371 0.61 -0.6 4,031,177 - Psyr_3371 0.61 +0.5 4,031,177 - Psyr_3371 0.61 -0.3 4,031,177 - Psyr_3371 0.61 +1.1 4,031,177 - Psyr_3371 0.61 -0.3 4,031,228 + Psyr_3371 0.68 -0.2 4,031,230 + Psyr_3371 0.68 -0.4 4,031,230 + Psyr_3371 0.68 +0.0 4,031,230 + Psyr_3371 0.68 -0.2 4,031,231 - Psyr_3371 0.68 +0.3 4,031,231 - Psyr_3371 0.68 +0.0 4,031,231 - Psyr_3371 0.68 +0.5 4,031,231 - Psyr_3371 0.68 -0.7 4,031,453 + -0.3 4,031,453 + -0.8 4,031,459 + -2.5 4,031,533 + Psyr_3372 0.10 +0.1 4,031,534 - Psyr_3372 0.10 +0.8 4,031,607 - Psyr_3372 0.22 -1.1 4,031,699 + Psyr_3372 0.37 -0.3 4,031,724 + Psyr_3372 0.41 -0.8 4,031,724 + Psyr_3372 0.41 -0.0 4,031,724 + Psyr_3372 0.41 +0.6 4,031,724 + Psyr_3372 0.41 +0.2 4,031,724 + Psyr_3372 0.41 +0.8 4,031,724 + Psyr_3372 0.41 +0.6 4,031,725 - Psyr_3372 0.41 +1.1 4,031,725 - Psyr_3372 0.41 -1.5 4,031,725 - Psyr_3372 0.41 -0.3 4,031,725 - Psyr_3372 0.41 -0.1 4,031,725 - Psyr_3372 0.41 +1.5 4,031,725 - Psyr_3372 0.41 +1.4 4,031,871 + Psyr_3372 0.64 +1.5 4,031,872 - Psyr_3372 0.65 -1.6 4,031,872 - Psyr_3372 0.65 +0.7 4,031,872 - Psyr_3372 0.65 +0.5 4,031,994 + Psyr_3372 0.84 -1.9 4,032,019 + Psyr_3372 0.88 -0.9 4,032,019 + Psyr_3372 0.88 +0.9 4,032,019 + Psyr_3372 0.88 +0.5 4,032,019 + Psyr_3372 0.88 -1.0 4,032,019 + Psyr_3372 0.88 +0.3 4,032,019 + Psyr_3372 0.88 -0.3 4,032,019 + Psyr_3372 0.88 +0.5 4,032,020 - Psyr_3372 0.88 -0.8 4,032,020 - Psyr_3372 0.88 -0.1 4,032,020 - Psyr_3372 0.88 +1.1 4,032,020 - Psyr_3372 0.88 -1.2 4,032,020 - Psyr_3372 0.88 -0.8 4,032,167 + +1.1 4,032,167 + -0.2 4,032,167 + +0.4 4,032,167 + +0.5 4,032,168 - -0.6 4,032,168 - +0.5 4,032,168 - +2.2 4,032,168 - +0.4 4,032,238 + Psyr_3373 0.12 +1.7 4,032,341 + Psyr_3373 0.29 +1.8 4,032,341 + Psyr_3373 0.29 -0.1 4,032,341 + Psyr_3373 0.29 +2.1 4,032,341 + Psyr_3373 0.29 +0.5 4,032,341 + Psyr_3373 0.29 -0.4 4,032,342 - Psyr_3373 0.29 +0.9 4,032,342 - Psyr_3373 0.29 +0.9 4,032,342 - Psyr_3373 0.29 +0.1 4,032,342 - Psyr_3373 0.29 +0.4 4,032,342 - Psyr_3373 0.29 +1.5 4,032,609 + Psyr_3373 0.73 +2.1 4,032,750 + -0.5 4,032,751 - -0.5 4,032,751 - +1.1 4,032,751 - -1.2 4,032,753 + -0.1 4,032,753 + -0.1 4,032,775 + +1.8 4,032,776 - +1.9 4,032,829 + -0.2 4,032,829 + -0.5 4,032,829 + -0.5 4,032,829 + +1.5 4,032,830 - -0.1 4,032,830 - +2.5 4,032,830 - +0.4 4,032,888 - +0.5 4,032,947 + -1.1 4,032,955 + -0.0 4,032,956 - +1.7 4,032,956 - +0.5 4,033,230 + Psyr_3374 0.42 +0.5 4,033,301 - Psyr_3374 0.52 -1.3 4,033,442 - Psyr_3374 0.72 -0.5 4,033,442 - Psyr_3374 0.72 -0.1 4,033,442 - Psyr_3374 0.72 -1.6 4,033,442 - Psyr_3374 0.72 +1.0 4,033,590 + -0.1 4,033,590 + +0.3 4,033,591 - +1.4 4,033,694 + +1.5 4,033,695 - -0.3 4,033,695 - -0.1 4,033,700 + -0.3
Or see this region's nucleotide sequence