Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3193 and Psyr_3194 overlap by 8 nucleotides Psyr_3194 and Psyr_3195 are separated by 186 nucleotides Psyr_3195 and Psyr_3196 are separated by 528 nucleotides
Psyr_3193: Psyr_3193 - Transcription factor jumonji, jmjC, at 3,822,877 to 3,824,043
_3193
Psyr_3194: Psyr_3194 - GCN5-related N-acetyltransferase, at 3,824,036 to 3,824,458
_3194
Psyr_3195: Psyr_3195 - NolW-like protein, at 3,824,645 to 3,825,388
_3195
Psyr_3196: Psyr_3196 - isocitrate lyase, at 3,825,917 to 3,827,242
_3196
Position (kb)
3824
3825
3826 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3824.042 kb on - strand at 3824.042 kb on - strand at 3824.042 kb on - strand at 3824.052 kb on + strand at 3824.052 kb on + strand at 3824.053 kb on - strand at 3824.053 kb on - strand at 3824.053 kb on - strand at 3824.053 kb on - strand at 3824.053 kb on - strand at 3824.186 kb on + strand, within Psyr_3194 at 3824.375 kb on + strand, within Psyr_3194 at 3824.440 kb on + strand at 3824.493 kb on - strand at 3824.493 kb on - strand at 3824.496 kb on + strand at 3824.496 kb on + strand at 3824.497 kb on - strand at 3824.497 kb on - strand at 3824.653 kb on - strand at 3824.741 kb on + strand, within Psyr_3195 at 3825.080 kb on + strand, within Psyr_3195 at 3825.080 kb on + strand, within Psyr_3195 at 3825.081 kb on - strand, within Psyr_3195 at 3825.081 kb on - strand, within Psyr_3195 at 3825.117 kb on + strand, within Psyr_3195 at 3825.118 kb on - strand, within Psyr_3195 at 3825.131 kb on + strand, within Psyr_3195 at 3825.131 kb on + strand, within Psyr_3195 at 3825.131 kb on + strand, within Psyr_3195 at 3825.131 kb on + strand, within Psyr_3195 at 3825.132 kb on - strand, within Psyr_3195 at 3825.366 kb on + strand at 3825.412 kb on - strand at 3825.412 kb on - strand at 3825.455 kb on + strand at 3825.455 kb on + strand at 3825.455 kb on + strand at 3825.456 kb on - strand at 3825.459 kb on + strand at 3825.460 kb on - strand at 3825.460 kb on - strand at 3825.667 kb on + strand at 3825.796 kb on - strand at 3825.840 kb on + strand at 3825.840 kb on + strand at 3825.841 kb on - strand at 3825.841 kb on - strand at 3825.841 kb on - strand at 3825.896 kb on + strand at 3825.896 kb on + strand at 3825.896 kb on + strand at 3825.896 kb on + strand at 3825.897 kb on - strand at 3825.897 kb on - strand at 3825.903 kb on - strand at 3825.903 kb on - strand at 3825.911 kb on - strand at 3825.943 kb on - strand at 3826.001 kb on + strand at 3826.001 kb on + strand at 3826.002 kb on - strand at 3826.002 kb on - strand at 3826.002 kb on - strand at 3826.205 kb on + strand, within Psyr_3196 at 3826.205 kb on + strand, within Psyr_3196 at 3826.205 kb on + strand, within Psyr_3196 at 3826.206 kb on - strand, within Psyr_3196 at 3826.206 kb on - strand, within Psyr_3196 at 3826.206 kb on - strand at 3826.206 kb on - strand, within Psyr_3196 at 3826.206 kb on - strand, within Psyr_3196 at 3826.206 kb on - strand, within Psyr_3196 at 3826.206 kb on - strand, within Psyr_3196 at 3826.379 kb on + strand, within Psyr_3196 at 3826.379 kb on + strand, within Psyr_3196 at 3826.379 kb on + strand, within Psyr_3196 at 3826.380 kb on - strand, within Psyr_3196 at 3826.380 kb on - strand, within Psyr_3196 at 3826.380 kb on - strand, within Psyr_3196 at 3826.388 kb on + strand, within Psyr_3196
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 3,824,042 - +0.4 3,824,042 - -0.8 3,824,042 - -0.6 3,824,052 + +2.4 3,824,052 + +0.2 3,824,053 - +0.9 3,824,053 - +0.7 3,824,053 - +0.5 3,824,053 - -0.2 3,824,053 - +1.0 3,824,186 + Psyr_3194 0.35 +0.7 3,824,375 + Psyr_3194 0.80 -1.9 3,824,440 + +0.2 3,824,493 - -1.0 3,824,493 - +2.2 3,824,496 + -0.1 3,824,496 + +0.7 3,824,497 - -1.6 3,824,497 - +1.4 3,824,653 - -0.1 3,824,741 + Psyr_3195 0.13 +0.6 3,825,080 + Psyr_3195 0.58 -1.5 3,825,080 + Psyr_3195 0.58 +2.6 3,825,081 - Psyr_3195 0.59 -0.1 3,825,081 - Psyr_3195 0.59 +1.2 3,825,117 + Psyr_3195 0.63 -1.0 3,825,118 - Psyr_3195 0.64 +2.7 3,825,131 + Psyr_3195 0.65 +0.6 3,825,131 + Psyr_3195 0.65 +0.1 3,825,131 + Psyr_3195 0.65 +0.8 3,825,131 + Psyr_3195 0.65 +1.5 3,825,132 - Psyr_3195 0.65 +1.4 3,825,366 + +1.4 3,825,412 - +0.2 3,825,412 - +1.4 3,825,455 + -1.6 3,825,455 + +1.4 3,825,455 + +1.7 3,825,456 - -0.6 3,825,459 + -0.4 3,825,460 - -0.3 3,825,460 - -3.4 3,825,667 + +0.4 3,825,796 - -0.6 3,825,840 + -0.0 3,825,840 + +0.3 3,825,841 - +0.8 3,825,841 - -0.3 3,825,841 - -0.4 3,825,896 + +1.0 3,825,896 + +0.1 3,825,896 + -0.3 3,825,896 + +1.2 3,825,897 - -0.5 3,825,897 - +0.3 3,825,903 - +1.0 3,825,903 - +1.2 3,825,911 - -1.3 3,825,943 - -0.7 3,826,001 + -0.5 3,826,001 + +1.0 3,826,002 - +0.4 3,826,002 - +0.4 3,826,002 - +3.0 3,826,205 + Psyr_3196 0.22 -0.3 3,826,205 + Psyr_3196 0.22 -0.7 3,826,205 + Psyr_3196 0.22 +1.0 3,826,206 - Psyr_3196 0.22 +0.4 3,826,206 - Psyr_3196 0.22 +1.6 3,826,206 - +0.6 3,826,206 - Psyr_3196 0.22 -0.0 3,826,206 - Psyr_3196 0.22 +1.7 3,826,206 - Psyr_3196 0.22 +0.5 3,826,206 - Psyr_3196 0.22 -0.1 3,826,379 + Psyr_3196 0.35 +1.9 3,826,379 + Psyr_3196 0.35 +1.0 3,826,379 + Psyr_3196 0.35 +1.0 3,826,380 - Psyr_3196 0.35 +0.2 3,826,380 - Psyr_3196 0.35 +0.9 3,826,380 - Psyr_3196 0.35 -1.0 3,826,388 + Psyr_3196 0.36 -0.1
Or see this region's nucleotide sequence