Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2467 and Psyr_2468 overlap by 4 nucleotides Psyr_2468 and Psyr_2469 are separated by 14 nucleotides Psyr_2469 and Psyr_2470 are separated by 13 nucleotides
Psyr_2467: Psyr_2467 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 2,858,815 to 2,860,764
_2467
Psyr_2468: Psyr_2468 - Enoyl-CoA hydratase/isomerase, at 2,860,761 to 2,861,636
_2468
Psyr_2469: Psyr_2469 - Propionyl-CoA carboxylase, at 2,861,651 to 2,863,258
_2469
Psyr_2470: Psyr_2470 - isovaleryl-CoA dehydrogenase, at 2,863,272 to 2,864,435
_2470
Position (kb)
2861
2862
2863
2864 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2861.141 kb on - strand, within Psyr_2468 at 2861.347 kb on + strand, within Psyr_2468 at 2861.347 kb on + strand, within Psyr_2468 at 2861.347 kb on + strand, within Psyr_2468 at 2861.348 kb on - strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.393 kb on - strand, within Psyr_2468 at 2861.393 kb on - strand, within Psyr_2468 at 2861.497 kb on - strand, within Psyr_2468 at 2861.497 kb on - strand, within Psyr_2468 at 2861.611 kb on - strand at 2861.678 kb on - strand at 2861.784 kb on + strand at 2861.805 kb on + strand at 2861.957 kb on + strand, within Psyr_2469 at 2861.958 kb on - strand, within Psyr_2469 at 2861.994 kb on + strand, within Psyr_2469 at 2862.105 kb on + strand, within Psyr_2469 at 2862.105 kb on + strand, within Psyr_2469 at 2862.105 kb on + strand, within Psyr_2469 at 2862.106 kb on - strand, within Psyr_2469 at 2862.106 kb on - strand, within Psyr_2469 at 2862.350 kb on + strand, within Psyr_2469 at 2862.351 kb on - strand, within Psyr_2469 at 2862.351 kb on - strand, within Psyr_2469 at 2862.516 kb on + strand, within Psyr_2469 at 2862.728 kb on - strand, within Psyr_2469 at 2862.837 kb on + strand, within Psyr_2469 at 2862.837 kb on + strand, within Psyr_2469 at 2862.837 kb on + strand, within Psyr_2469 at 2862.837 kb on + strand, within Psyr_2469 at 2862.838 kb on - strand, within Psyr_2469 at 2862.838 kb on - strand, within Psyr_2469 at 2862.838 kb on - strand, within Psyr_2469 at 2862.909 kb on + strand, within Psyr_2469 at 2863.014 kb on + strand, within Psyr_2469 at 2863.015 kb on - strand, within Psyr_2469 at 2863.016 kb on + strand, within Psyr_2469 at 2863.016 kb on + strand, within Psyr_2469 at 2863.016 kb on + strand, within Psyr_2469 at 2863.017 kb on - strand, within Psyr_2469 at 2863.017 kb on - strand, within Psyr_2469 at 2863.082 kb on - strand, within Psyr_2469 at 2863.082 kb on - strand, within Psyr_2469 at 2863.345 kb on + strand at 2863.394 kb on - strand, within Psyr_2470 at 2863.394 kb on - strand, within Psyr_2470 at 2863.394 kb on - strand, within Psyr_2470 at 2863.456 kb on + strand, within Psyr_2470 at 2863.456 kb on + strand, within Psyr_2470 at 2863.522 kb on + strand, within Psyr_2470 at 2863.522 kb on + strand, within Psyr_2470 at 2863.523 kb on - strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.555 kb on + strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.556 kb on - strand, within Psyr_2470 at 2863.578 kb on + strand, within Psyr_2470 at 2863.578 kb on + strand, within Psyr_2470 at 2863.579 kb on - strand, within Psyr_2470 at 2863.630 kb on + strand, within Psyr_2470 at 2863.631 kb on - strand, within Psyr_2470 at 2863.687 kb on - strand, within Psyr_2470 at 2863.915 kb on + strand, within Psyr_2470 at 2863.915 kb on + strand, within Psyr_2470 at 2863.916 kb on - strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.924 kb on + strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470 at 2863.925 kb on - strand, within Psyr_2470
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 2,861,141 - Psyr_2468 0.43 -2.4 2,861,347 + Psyr_2468 0.67 -0.5 2,861,347 + Psyr_2468 0.67 +0.1 2,861,347 + Psyr_2468 0.67 +0.1 2,861,348 - Psyr_2468 0.67 -1.9 2,861,392 + Psyr_2468 0.72 -0.4 2,861,393 - Psyr_2468 0.72 +1.1 2,861,393 - Psyr_2468 0.72 -1.2 2,861,497 - Psyr_2468 0.84 -0.2 2,861,497 - Psyr_2468 0.84 -0.6 2,861,611 - +1.2 2,861,678 - -1.0 2,861,784 + +0.2 2,861,805 + -1.9 2,861,957 + Psyr_2469 0.19 -0.6 2,861,958 - Psyr_2469 0.19 +0.7 2,861,994 + Psyr_2469 0.21 +0.8 2,862,105 + Psyr_2469 0.28 +0.1 2,862,105 + Psyr_2469 0.28 -1.0 2,862,105 + Psyr_2469 0.28 -0.3 2,862,106 - Psyr_2469 0.28 -0.3 2,862,106 - Psyr_2469 0.28 -1.2 2,862,350 + Psyr_2469 0.43 -0.9 2,862,351 - Psyr_2469 0.44 -0.7 2,862,351 - Psyr_2469 0.44 +0.4 2,862,516 + Psyr_2469 0.54 +0.8 2,862,728 - Psyr_2469 0.67 -0.9 2,862,837 + Psyr_2469 0.74 -0.4 2,862,837 + Psyr_2469 0.74 -1.6 2,862,837 + Psyr_2469 0.74 +1.3 2,862,837 + Psyr_2469 0.74 +1.0 2,862,838 - Psyr_2469 0.74 -0.3 2,862,838 - Psyr_2469 0.74 +0.2 2,862,838 - Psyr_2469 0.74 +0.3 2,862,909 + Psyr_2469 0.78 +1.4 2,863,014 + Psyr_2469 0.85 -0.7 2,863,015 - Psyr_2469 0.85 +2.0 2,863,016 + Psyr_2469 0.85 +0.5 2,863,016 + Psyr_2469 0.85 -0.6 2,863,016 + Psyr_2469 0.85 -0.6 2,863,017 - Psyr_2469 0.85 +0.7 2,863,017 - Psyr_2469 0.85 -0.3 2,863,082 - Psyr_2469 0.89 +0.3 2,863,082 - Psyr_2469 0.89 +0.3 2,863,345 + +0.5 2,863,394 - Psyr_2470 0.10 +0.4 2,863,394 - Psyr_2470 0.10 +1.5 2,863,394 - Psyr_2470 0.10 -0.4 2,863,456 + Psyr_2470 0.16 -0.8 2,863,456 + Psyr_2470 0.16 -0.3 2,863,522 + Psyr_2470 0.21 +0.4 2,863,522 + Psyr_2470 0.21 +0.9 2,863,523 - Psyr_2470 0.22 -0.6 2,863,555 + Psyr_2470 0.24 -0.9 2,863,555 + Psyr_2470 0.24 -0.7 2,863,555 + Psyr_2470 0.24 +0.2 2,863,555 + Psyr_2470 0.24 +0.1 2,863,555 + Psyr_2470 0.24 -1.7 2,863,555 + Psyr_2470 0.24 -0.6 2,863,555 + Psyr_2470 0.24 -0.9 2,863,555 + Psyr_2470 0.24 +0.8 2,863,556 - Psyr_2470 0.24 -0.3 2,863,556 - Psyr_2470 0.24 -0.1 2,863,556 - Psyr_2470 0.24 +1.0 2,863,556 - Psyr_2470 0.24 -0.7 2,863,556 - Psyr_2470 0.24 +1.7 2,863,556 - Psyr_2470 0.24 +0.1 2,863,556 - Psyr_2470 0.24 +0.4 2,863,556 - Psyr_2470 0.24 -0.2 2,863,556 - Psyr_2470 0.24 +0.5 2,863,556 - Psyr_2470 0.24 +0.5 2,863,556 - Psyr_2470 0.24 -0.2 2,863,556 - Psyr_2470 0.24 +0.8 2,863,578 + Psyr_2470 0.26 +1.3 2,863,578 + Psyr_2470 0.26 +0.3 2,863,579 - Psyr_2470 0.26 -0.4 2,863,630 + Psyr_2470 0.31 +0.4 2,863,631 - Psyr_2470 0.31 -1.2 2,863,687 - Psyr_2470 0.36 +0.3 2,863,915 + Psyr_2470 0.55 -0.2 2,863,915 + Psyr_2470 0.55 +0.6 2,863,916 - Psyr_2470 0.55 -0.3 2,863,924 + Psyr_2470 0.56 +0.1 2,863,924 + Psyr_2470 0.56 +0.0 2,863,924 + Psyr_2470 0.56 -0.2 2,863,924 + Psyr_2470 0.56 +1.4 2,863,924 + Psyr_2470 0.56 -0.4 2,863,924 + Psyr_2470 0.56 +0.9 2,863,925 - Psyr_2470 0.56 -0.7 2,863,925 - Psyr_2470 0.56 -3.3 2,863,925 - Psyr_2470 0.56 -0.5 2,863,925 - Psyr_2470 0.56 -1.3 2,863,925 - Psyr_2470 0.56 -0.1 2,863,925 - Psyr_2470 0.56 -0.4 2,863,925 - Psyr_2470 0.56 -0.6 2,863,925 - Psyr_2470 0.56 +0.0
Or see this region's nucleotide sequence