Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2212 and Psyr_2213 are separated by 134 nucleotides Psyr_2213 and Psyr_2214 are separated by 623 nucleotides
Psyr_2212: Psyr_2212 - Arginase/agmatinase/formiminoglutamase, at 2,570,727 to 2,571,617
_2212
Psyr_2213: Psyr_2213 - transcriptional regulator, AraC family, at 2,571,752 to 2,572,681
_2213
Psyr_2214: Psyr_2214 - PAS, at 2,573,305 to 2,575,437
_2214
Position (kb)
2571
2572
2573 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2570.879 kb on + strand, within Psyr_2212 at 2571.133 kb on + strand, within Psyr_2212 at 2571.133 kb on + strand, within Psyr_2212 at 2571.133 kb on + strand, within Psyr_2212 at 2571.133 kb on + strand, within Psyr_2212 at 2571.133 kb on + strand, within Psyr_2212 at 2571.133 kb on + strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.134 kb on - strand, within Psyr_2212 at 2571.313 kb on + strand, within Psyr_2212 at 2571.753 kb on + strand at 2572.130 kb on + strand, within Psyr_2213 at 2572.130 kb on + strand, within Psyr_2213 at 2572.130 kb on + strand, within Psyr_2213 at 2572.130 kb on + strand, within Psyr_2213 at 2572.525 kb on + strand, within Psyr_2213 at 2572.526 kb on - strand, within Psyr_2213 at 2572.526 kb on - strand, within Psyr_2213 at 2572.602 kb on + strand at 2572.603 kb on - strand at 2572.765 kb on + strand at 2572.766 kb on - strand at 2572.796 kb on + strand at 2572.797 kb on - strand at 2572.797 kb on - strand at 2572.818 kb on + strand at 2572.824 kb on + strand at 2572.824 kb on + strand at 2572.828 kb on + strand at 2572.828 kb on + strand at 2572.828 kb on + strand at 2572.828 kb on + strand at 2572.828 kb on + strand at 2572.829 kb on - strand at 2572.829 kb on - strand at 2572.829 kb on - strand at 2572.829 kb on - strand at 2572.950 kb on + strand at 2572.950 kb on + strand at 2572.951 kb on - strand at 2572.953 kb on + strand at 2572.996 kb on + strand at 2572.997 kb on - strand at 2573.096 kb on - strand at 2573.096 kb on - strand at 2573.101 kb on + strand at 2573.102 kb on - strand at 2573.108 kb on - strand at 2573.108 kb on - strand at 2573.108 kb on - strand at 2573.213 kb on + strand at 2573.261 kb on + strand at 2573.261 kb on + strand at 2573.262 kb on - strand at 2573.269 kb on + strand at 2573.270 kb on - strand at 2573.270 kb on - strand at 2573.270 kb on - strand at 2573.270 kb on - strand at 2573.277 kb on + strand at 2573.277 kb on + strand at 2573.278 kb on - strand at 2573.285 kb on + strand at 2573.286 kb on - strand at 2573.286 kb on - strand at 2573.537 kb on - strand, within Psyr_2214 at 2573.588 kb on - strand, within Psyr_2214 at 2573.635 kb on + strand, within Psyr_2214 at 2573.635 kb on + strand, within Psyr_2214 at 2573.635 kb on + strand, within Psyr_2214 at 2573.636 kb on - strand, within Psyr_2214 at 2573.637 kb on + strand, within Psyr_2214 at 2573.637 kb on + strand, within Psyr_2214
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 2,570,879 + Psyr_2212 0.17 +2.1 2,571,133 + Psyr_2212 0.46 -1.6 2,571,133 + Psyr_2212 0.46 -0.2 2,571,133 + Psyr_2212 0.46 +0.5 2,571,133 + Psyr_2212 0.46 -0.5 2,571,133 + Psyr_2212 0.46 -0.1 2,571,133 + Psyr_2212 0.46 -0.5 2,571,134 - Psyr_2212 0.46 +0.8 2,571,134 - Psyr_2212 0.46 -0.2 2,571,134 - Psyr_2212 0.46 -0.2 2,571,134 - Psyr_2212 0.46 -0.5 2,571,134 - Psyr_2212 0.46 -0.0 2,571,134 - Psyr_2212 0.46 -0.7 2,571,134 - Psyr_2212 0.46 -0.2 2,571,134 - Psyr_2212 0.46 -0.2 2,571,134 - Psyr_2212 0.46 -0.7 2,571,134 - Psyr_2212 0.46 -0.2 2,571,313 + Psyr_2212 0.66 -0.1 2,571,753 + +0.7 2,572,130 + Psyr_2213 0.41 -2.6 2,572,130 + Psyr_2213 0.41 -3.0 2,572,130 + Psyr_2213 0.41 -0.5 2,572,130 + Psyr_2213 0.41 -0.7 2,572,525 + Psyr_2213 0.83 +0.0 2,572,526 - Psyr_2213 0.83 -0.5 2,572,526 - Psyr_2213 0.83 -0.3 2,572,602 + -0.1 2,572,603 - -0.3 2,572,765 + +1.6 2,572,766 - -0.1 2,572,796 + +0.2 2,572,797 - +1.3 2,572,797 - -0.2 2,572,818 + +0.7 2,572,824 + +0.3 2,572,824 + -0.3 2,572,828 + -1.6 2,572,828 + +0.3 2,572,828 + +1.0 2,572,828 + +1.2 2,572,828 + -0.3 2,572,829 - +0.1 2,572,829 - -0.8 2,572,829 - +0.7 2,572,829 - -0.1 2,572,950 + +0.5 2,572,950 + -0.3 2,572,951 - +0.4 2,572,953 + +0.5 2,572,996 + -0.5 2,572,997 - -1.7 2,573,096 - -0.0 2,573,096 - +0.1 2,573,101 + +0.5 2,573,102 - -1.9 2,573,108 - -1.1 2,573,108 - -0.6 2,573,108 - -0.8 2,573,213 + -1.1 2,573,261 + -0.5 2,573,261 + +3.6 2,573,262 - -0.3 2,573,269 + +2.5 2,573,270 - -1.1 2,573,270 - -0.0 2,573,270 - +0.2 2,573,270 - +0.8 2,573,277 + +0.6 2,573,277 + -0.3 2,573,278 - -0.9 2,573,285 + +0.2 2,573,286 - -1.0 2,573,286 - +1.6 2,573,537 - Psyr_2214 0.11 +0.3 2,573,588 - Psyr_2214 0.13 -1.1 2,573,635 + Psyr_2214 0.15 +0.3 2,573,635 + Psyr_2214 0.15 +0.1 2,573,635 + Psyr_2214 0.15 +0.7 2,573,636 - Psyr_2214 0.16 -1.5 2,573,637 + Psyr_2214 0.16 -1.7 2,573,637 + Psyr_2214 0.16 +0.3
Or see this region's nucleotide sequence