Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2199 and Psyr_2200 overlap by 4 nucleotides Psyr_2200 and Psyr_2201 are separated by 22 nucleotides Psyr_2201 and Psyr_2202 are separated by 2 nucleotides Psyr_2202 and Psyr_2203 overlap by 4 nucleotides
Psyr_2199: Psyr_2199 - Urease accessory protein UreF, at 2,556,940 to 2,557,671
_2199
Psyr_2200: Psyr_2200 - UreE urease accessory, N-terminal, at 2,557,668 to 2,558,108
_2200
Psyr_2201: Psyr_2201 - amino acid/amide ABC transporter ATP-binding protein 2, HAAT family, at 2,558,131 to 2,558,820
_2201
Psyr_2202: Psyr_2202 - ABC transporter, at 2,558,823 to 2,559,560
_2202
Psyr_2203: Psyr_2203 - amino acid/amide ABC transporter membrane protein 2, HAAT family, at 2,559,557 to 2,560,699
_2203
Position (kb)
2558
2559 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2557.801 kb on + strand, within Psyr_2200 at 2557.849 kb on + strand, within Psyr_2200 at 2557.850 kb on - strand, within Psyr_2200 at 2558.474 kb on + strand, within Psyr_2201 at 2558.486 kb on + strand, within Psyr_2201 at 2558.486 kb on + strand, within Psyr_2201 at 2558.487 kb on - strand, within Psyr_2201 at 2558.609 kb on + strand, within Psyr_2201 at 2558.792 kb on + strand at 2558.792 kb on + strand at 2558.792 kb on + strand at 2558.792 kb on + strand at 2558.793 kb on - strand at 2558.885 kb on - strand at 2559.481 kb on + strand, within Psyr_2202 at 2559.481 kb on + strand, within Psyr_2202 at 2559.481 kb on + strand, within Psyr_2202 at 2559.481 kb on + strand, within Psyr_2202 at 2559.482 kb on - strand, within Psyr_2202 at 2559.482 kb on - strand, within Psyr_2202 at 2559.654 kb on + strand at 2559.655 kb on - strand at 2559.655 kb on - strand at 2559.762 kb on + strand, within Psyr_2203 at 2559.763 kb on - strand, within Psyr_2203
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 2,557,801 + Psyr_2200 0.30 -0.8 2,557,849 + Psyr_2200 0.41 +3.5 2,557,850 - Psyr_2200 0.41 -0.3 2,558,474 + Psyr_2201 0.50 -1.5 2,558,486 + Psyr_2201 0.51 +0.4 2,558,486 + Psyr_2201 0.51 -1.2 2,558,487 - Psyr_2201 0.52 -2.5 2,558,609 + Psyr_2201 0.69 +0.1 2,558,792 + +1.3 2,558,792 + -0.1 2,558,792 + +0.2 2,558,792 + +0.2 2,558,793 - -0.9 2,558,885 - +3.9 2,559,481 + Psyr_2202 0.89 +1.4 2,559,481 + Psyr_2202 0.89 +0.1 2,559,481 + Psyr_2202 0.89 +0.1 2,559,481 + Psyr_2202 0.89 +0.9 2,559,482 - Psyr_2202 0.89 +0.3 2,559,482 - Psyr_2202 0.89 -0.7 2,559,654 + -2.6 2,559,655 - -0.2 2,559,655 - -1.4 2,559,762 + Psyr_2203 0.18 +2.5 2,559,763 - Psyr_2203 0.18 -0.7
Or see this region's nucleotide sequence