Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1401 and Psyr_1402 overlap by 4 nucleotides Psyr_1402 and Psyr_1403 are separated by 64 nucleotides
Psyr_1401: Psyr_1401 - Histidine triad (HIT) protein, at 1,588,962 to 1,589,393
_1401
Psyr_1402: Psyr_1402 - SlyX, at 1,589,390 to 1,589,596
_1402
Psyr_1403: Psyr_1403 - cold-shock DNA-binding protein family, at 1,589,661 to 1,589,882
_1403
Position (kb)
1589
1590 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1588.597 kb on + strand at 1588.597 kb on + strand at 1588.597 kb on + strand at 1588.597 kb on + strand at 1588.598 kb on - strand at 1588.598 kb on - strand at 1588.616 kb on + strand at 1588.616 kb on + strand at 1588.616 kb on + strand at 1588.616 kb on + strand at 1588.616 kb on + strand at 1588.617 kb on - strand at 1588.617 kb on - strand at 1588.718 kb on + strand at 1588.718 kb on + strand at 1588.718 kb on + strand at 1588.718 kb on + strand at 1588.718 kb on + strand at 1588.718 kb on + strand at 1588.718 kb on + strand at 1588.718 kb on + strand at 1588.718 kb on + strand at 1588.719 kb on - strand at 1588.719 kb on - strand at 1588.719 kb on - strand at 1588.719 kb on - strand at 1588.741 kb on + strand at 1588.750 kb on + strand at 1588.808 kb on + strand at 1588.808 kb on + strand at 1588.808 kb on + strand at 1588.809 kb on - strand at 1588.818 kb on - strand at 1588.818 kb on - strand at 1588.884 kb on + strand at 1588.884 kb on + strand at 1588.884 kb on + strand at 1588.885 kb on - strand at 1588.887 kb on + strand at 1588.958 kb on + strand at 1588.995 kb on - strand at 1589.186 kb on + strand, within Psyr_1401 at 1589.187 kb on - strand, within Psyr_1401 at 1589.231 kb on + strand, within Psyr_1401 at 1589.231 kb on + strand, within Psyr_1401 at 1589.231 kb on + strand, within Psyr_1401 at 1589.231 kb on + strand, within Psyr_1401 at 1589.231 kb on + strand, within Psyr_1401 at 1589.231 kb on + strand, within Psyr_1401 at 1589.231 kb on + strand, within Psyr_1401 at 1589.232 kb on - strand, within Psyr_1401 at 1589.232 kb on - strand, within Psyr_1401 at 1589.307 kb on + strand, within Psyr_1401 at 1589.308 kb on - strand, within Psyr_1401 at 1589.308 kb on - strand, within Psyr_1401 at 1589.309 kb on + strand, within Psyr_1401 at 1589.309 kb on + strand, within Psyr_1401 at 1589.309 kb on + strand, within Psyr_1401 at 1589.309 kb on + strand, within Psyr_1401 at 1589.310 kb on - strand, within Psyr_1401 at 1589.310 kb on - strand, within Psyr_1401 at 1589.310 kb on - strand, within Psyr_1401 at 1589.446 kb on + strand, within Psyr_1402 at 1589.447 kb on - strand, within Psyr_1402 at 1589.475 kb on + strand, within Psyr_1402 at 1589.591 kb on + strand at 1589.591 kb on + strand at 1589.857 kb on - strand, within Psyr_1403 at 1589.915 kb on - strand at 1589.915 kb on - strand at 1589.915 kb on - strand at 1590.036 kb on + strand at 1590.036 kb on + strand at 1590.201 kb on - strand at 1590.210 kb on - strand at 1590.210 kb on - strand at 1590.273 kb on - strand at 1590.298 kb on - strand at 1590.306 kb on + strand at 1590.307 kb on - strand at 1590.313 kb on + strand at 1590.371 kb on + strand at 1590.371 kb on + strand at 1590.372 kb on - strand at 1590.372 kb on - strand at 1590.372 kb on - strand at 1590.414 kb on + strand at 1590.414 kb on + strand at 1590.418 kb on + strand at 1590.457 kb on - strand at 1590.500 kb on + strand at 1590.501 kb on - strand at 1590.501 kb on - strand at 1590.503 kb on + strand at 1590.503 kb on + strand at 1590.504 kb on - strand at 1590.504 kb on - strand at 1590.504 kb on - strand at 1590.550 kb on + strand at 1590.550 kb on + strand at 1590.550 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 1,588,597 + -0.2 1,588,597 + -0.4 1,588,597 + +1.1 1,588,597 + -0.7 1,588,598 - +1.4 1,588,598 - -1.8 1,588,616 + -0.0 1,588,616 + +1.3 1,588,616 + +1.0 1,588,616 + -1.4 1,588,616 + +0.7 1,588,617 - +0.3 1,588,617 - +0.2 1,588,718 + -0.4 1,588,718 + +1.7 1,588,718 + -0.3 1,588,718 + +0.0 1,588,718 + -1.2 1,588,718 + -0.6 1,588,718 + -0.1 1,588,718 + +0.2 1,588,718 + +0.1 1,588,719 - -0.3 1,588,719 - -0.6 1,588,719 - +0.6 1,588,719 - -1.5 1,588,741 + +1.2 1,588,750 + +0.7 1,588,808 + -1.5 1,588,808 + -0.5 1,588,808 + -0.9 1,588,809 - +0.6 1,588,818 - -0.2 1,588,818 - -2.8 1,588,884 + -2.3 1,588,884 + -1.9 1,588,884 + -1.8 1,588,885 - +1.5 1,588,887 + -0.1 1,588,958 + +0.3 1,588,995 - +1.2 1,589,186 + Psyr_1401 0.52 -0.4 1,589,187 - Psyr_1401 0.52 -0.4 1,589,231 + Psyr_1401 0.62 -0.3 1,589,231 + Psyr_1401 0.62 +0.7 1,589,231 + Psyr_1401 0.62 +0.5 1,589,231 + Psyr_1401 0.62 -1.0 1,589,231 + Psyr_1401 0.62 -0.1 1,589,231 + Psyr_1401 0.62 -0.1 1,589,231 + Psyr_1401 0.62 -0.0 1,589,232 - Psyr_1401 0.62 +0.1 1,589,232 - Psyr_1401 0.62 +0.3 1,589,307 + Psyr_1401 0.80 +1.5 1,589,308 - Psyr_1401 0.80 -0.8 1,589,308 - Psyr_1401 0.80 +1.0 1,589,309 + Psyr_1401 0.80 -0.5 1,589,309 + Psyr_1401 0.80 -0.1 1,589,309 + Psyr_1401 0.80 -0.2 1,589,309 + Psyr_1401 0.80 +1.0 1,589,310 - Psyr_1401 0.81 -0.1 1,589,310 - Psyr_1401 0.81 +0.1 1,589,310 - Psyr_1401 0.81 -2.1 1,589,446 + Psyr_1402 0.27 +0.9 1,589,447 - Psyr_1402 0.28 +0.1 1,589,475 + Psyr_1402 0.41 +1.4 1,589,591 + -2.4 1,589,591 + +0.3 1,589,857 - Psyr_1403 0.88 -3.7 1,589,915 - +2.7 1,589,915 - +1.1 1,589,915 - -0.6 1,590,036 + +0.1 1,590,036 + -1.3 1,590,201 - -2.0 1,590,210 - -1.8 1,590,210 - -0.3 1,590,273 - -0.7 1,590,298 - +1.4 1,590,306 + -2.2 1,590,307 - -0.3 1,590,313 + -2.6 1,590,371 + -1.7 1,590,371 + -1.1 1,590,372 - -0.3 1,590,372 - -0.9 1,590,372 - -1.2 1,590,414 + -1.0 1,590,414 + -2.5 1,590,418 + -0.0 1,590,457 - +0.7 1,590,500 + -0.1 1,590,501 - -3.1 1,590,501 - -1.2 1,590,503 + -0.6 1,590,503 + +0.4 1,590,504 - -1.4 1,590,504 - -0.2 1,590,504 - +0.9 1,590,550 + -0.1 1,590,550 + -1.6 1,590,550 + +2.3
Or see this region's nucleotide sequence