Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1013 and Psyr_1014 are separated by 316 nucleotides Psyr_1014 and Psyr_1015 are separated by 184 nucleotides Psyr_1015 and Psyr_1016 are separated by 375 nucleotides
Psyr_1013: Psyr_1013 - hypothetical protein, at 1,147,555 to 1,147,875
_1013
Psyr_1014: Psyr_1014 - conserved domain protein, at 1,148,192 to 1,148,536
_1014
Psyr_1015: Psyr_1015 - Protein of unknown function DUF540, at 1,148,721 to 1,149,482
_1015
Psyr_1016: Psyr_1016 - Thioredoxin reductase, at 1,149,858 to 1,150,820
_1016
Position (kb) 1148
1149
1150 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1147.808 kb on + strand, within Psyr_1013 at 1147.858 kb on + strand at 1147.858 kb on + strand at 1147.859 kb on - strand at 1147.900 kb on - strand at 1147.900 kb on - strand at 1147.903 kb on + strand at 1147.903 kb on + strand at 1147.904 kb on - strand at 1147.907 kb on - strand at 1147.921 kb on + strand at 1147.940 kb on - strand at 1147.957 kb on + strand at 1147.957 kb on + strand at 1147.957 kb on + strand at 1147.957 kb on + strand at 1147.957 kb on + strand at 1147.958 kb on - strand at 1147.984 kb on - strand at 1148.054 kb on - strand at 1148.055 kb on + strand at 1148.055 kb on + strand at 1148.055 kb on + strand at 1148.055 kb on + strand at 1148.056 kb on - strand at 1148.056 kb on - strand at 1148.056 kb on - strand at 1148.057 kb on + strand at 1148.057 kb on + strand at 1148.057 kb on + strand at 1148.057 kb on + strand at 1148.058 kb on - strand at 1148.058 kb on - strand at 1148.058 kb on - strand at 1148.080 kb on + strand at 1148.080 kb on + strand at 1148.081 kb on - strand at 1148.081 kb on - strand at 1148.081 kb on - strand at 1148.082 kb on + strand at 1148.082 kb on + strand at 1148.082 kb on + strand at 1148.082 kb on + strand at 1148.082 kb on + strand at 1148.082 kb on + strand at 1148.082 kb on + strand at 1148.082 kb on + strand at 1148.083 kb on - strand at 1148.083 kb on - strand at 1148.083 kb on - strand at 1148.083 kb on - strand at 1148.083 kb on - strand at 1148.083 kb on - strand at 1148.083 kb on - strand at 1148.083 kb on - strand at 1148.084 kb on + strand at 1148.084 kb on + strand at 1148.084 kb on + strand at 1148.084 kb on + strand at 1148.084 kb on + strand at 1148.085 kb on - strand at 1148.091 kb on - strand at 1148.128 kb on + strand at 1148.129 kb on - strand at 1148.161 kb on + strand at 1148.274 kb on + strand, within Psyr_1014 at 1148.275 kb on - strand, within Psyr_1014 at 1148.280 kb on + strand, within Psyr_1014 at 1148.281 kb on - strand, within Psyr_1014 at 1148.316 kb on + strand, within Psyr_1014 at 1148.316 kb on + strand, within Psyr_1014 at 1148.418 kb on + strand, within Psyr_1014 at 1148.418 kb on + strand, within Psyr_1014 at 1148.418 kb on + strand, within Psyr_1014 at 1148.418 kb on + strand, within Psyr_1014 at 1148.418 kb on + strand, within Psyr_1014 at 1148.419 kb on - strand, within Psyr_1014 at 1148.419 kb on - strand, within Psyr_1014 at 1148.419 kb on - strand, within Psyr_1014 at 1148.419 kb on - strand, within Psyr_1014 at 1148.419 kb on - strand, within Psyr_1014 at 1148.419 kb on - strand, within Psyr_1014 at 1148.522 kb on + strand at 1148.523 kb on - strand at 1148.523 kb on - strand at 1148.541 kb on + strand at 1148.553 kb on + strand at 1148.553 kb on + strand at 1148.554 kb on - strand at 1148.621 kb on - strand at 1148.652 kb on + strand at 1148.652 kb on + strand at 1148.652 kb on + strand at 1148.652 kb on + strand at 1148.652 kb on + strand at 1148.652 kb on + strand at 1148.652 kb on + strand at 1148.652 kb on + strand at 1148.652 kb on + strand at 1148.653 kb on - strand at 1148.653 kb on - strand at 1148.653 kb on - strand at 1148.653 kb on - strand at 1148.653 kb on - strand at 1148.653 kb on - strand at 1148.653 kb on - strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.656 kb on + strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.657 kb on - strand at 1148.658 kb on + strand at 1148.658 kb on + strand at 1148.658 kb on + strand at 1148.658 kb on + strand at 1148.658 kb on + strand at 1148.658 kb on + strand at 1148.658 kb on + strand at 1148.658 kb on + strand at 1148.658 kb on + strand at 1148.658 kb on + strand at 1148.658 kb on + strand at 1148.659 kb on - strand at 1148.659 kb on - strand at 1148.659 kb on - strand at 1148.659 kb on - strand at 1148.659 kb on - strand at 1148.659 kb on - strand at 1148.659 kb on - strand at 1148.659 kb on - strand at 1148.845 kb on + strand, within Psyr_1015 at 1148.845 kb on + strand, within Psyr_1015 at 1148.845 kb on + strand, within Psyr_1015 at 1148.846 kb on - strand, within Psyr_1015 at 1149.058 kb on + strand, within Psyr_1015 at 1149.058 kb on + strand, within Psyr_1015 at 1149.058 kb on + strand, within Psyr_1015 at 1149.058 kb on + strand, within Psyr_1015 at 1149.058 kb on + strand, within Psyr_1015 at 1149.058 kb on + strand, within Psyr_1015 at 1149.059 kb on - strand, within Psyr_1015 at 1149.059 kb on - strand, within Psyr_1015 at 1149.142 kb on - strand, within Psyr_1015 at 1149.274 kb on + strand, within Psyr_1015 at 1149.274 kb on + strand, within Psyr_1015 at 1149.274 kb on + strand, within Psyr_1015 at 1149.274 kb on + strand, within Psyr_1015 at 1149.274 kb on + strand, within Psyr_1015 at 1149.274 kb on + strand, within Psyr_1015 at 1149.275 kb on - strand, within Psyr_1015 at 1149.275 kb on - strand, within Psyr_1015 at 1149.275 kb on - strand, within Psyr_1015 at 1149.275 kb on - strand, within Psyr_1015 at 1149.352 kb on + strand, within Psyr_1015 at 1149.352 kb on + strand, within Psyr_1015 at 1149.353 kb on - strand, within Psyr_1015 at 1149.353 kb on - strand, within Psyr_1015 at 1149.353 kb on - strand, within Psyr_1015 at 1149.397 kb on - strand, within Psyr_1015 at 1149.451 kb on + strand at 1149.649 kb on + strand at 1149.651 kb on + strand at 1149.651 kb on + strand at 1149.651 kb on + strand at 1149.652 kb on - strand at 1149.652 kb on - strand at 1149.652 kb on - strand at 1149.652 kb on - strand at 1149.652 kb on - strand at 1149.652 kb on - strand at 1149.662 kb on + strand at 1149.662 kb on + strand at 1149.662 kb on + strand at 1149.662 kb on + strand at 1149.663 kb on - strand at 1149.663 kb on - strand at 1149.663 kb on - strand at 1149.665 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 1,147,808 + Psyr_1013 0.79 -0.0 1,147,858 + -0.3 1,147,858 + -1.4 1,147,859 - +0.4 1,147,900 - -0.5 1,147,900 - -1.5 1,147,903 + -0.1 1,147,903 + +1.8 1,147,904 - -0.2 1,147,907 - -0.2 1,147,921 + +0.8 1,147,940 - +0.2 1,147,957 + -0.1 1,147,957 + -1.8 1,147,957 + +0.2 1,147,957 + -0.5 1,147,957 + +0.3 1,147,958 - -0.1 1,147,984 - -2.0 1,148,054 - -1.1 1,148,055 + -0.1 1,148,055 + +0.4 1,148,055 + -0.7 1,148,055 + +0.2 1,148,056 - +0.6 1,148,056 - -0.7 1,148,056 - -0.5 1,148,057 + +0.8 1,148,057 + +1.5 1,148,057 + -0.6 1,148,057 + +2.3 1,148,058 - -0.4 1,148,058 - -1.8 1,148,058 - +0.1 1,148,080 + -3.4 1,148,080 + -1.0 1,148,081 - -3.7 1,148,081 - -0.2 1,148,081 - +0.3 1,148,082 + +0.4 1,148,082 + +2.3 1,148,082 + -0.0 1,148,082 + -0.4 1,148,082 + +0.8 1,148,082 + -0.1 1,148,082 + +1.4 1,148,082 + -0.1 1,148,083 - -0.0 1,148,083 - +1.9 1,148,083 - +1.8 1,148,083 - -1.9 1,148,083 - -0.4 1,148,083 - -0.4 1,148,083 - -1.6 1,148,083 - +0.6 1,148,084 + -0.1 1,148,084 + +0.1 1,148,084 + +0.0 1,148,084 + -1.6 1,148,084 + -0.7 1,148,085 - -0.8 1,148,091 - -0.8 1,148,128 + -0.6 1,148,129 - +0.3 1,148,161 + +1.3 1,148,274 + Psyr_1014 0.24 +0.2 1,148,275 - Psyr_1014 0.24 -0.5 1,148,280 + Psyr_1014 0.26 -0.5 1,148,281 - Psyr_1014 0.26 +0.7 1,148,316 + Psyr_1014 0.36 -0.4 1,148,316 + Psyr_1014 0.36 -0.3 1,148,418 + Psyr_1014 0.66 -1.4 1,148,418 + Psyr_1014 0.66 -0.7 1,148,418 + Psyr_1014 0.66 -0.1 1,148,418 + Psyr_1014 0.66 +0.2 1,148,418 + Psyr_1014 0.66 -0.4 1,148,419 - Psyr_1014 0.66 -0.4 1,148,419 - Psyr_1014 0.66 -1.6 1,148,419 - Psyr_1014 0.66 -0.2 1,148,419 - Psyr_1014 0.66 +0.8 1,148,419 - Psyr_1014 0.66 +1.2 1,148,419 - Psyr_1014 0.66 +0.0 1,148,522 + -2.8 1,148,523 - +0.1 1,148,523 - +0.1 1,148,541 + +3.6 1,148,553 + -0.1 1,148,553 + +0.2 1,148,554 - +0.4 1,148,621 - +0.3 1,148,652 + +0.7 1,148,652 + -0.4 1,148,652 + +0.6 1,148,652 + +2.6 1,148,652 + -0.3 1,148,652 + -0.4 1,148,652 + -0.1 1,148,652 + +2.0 1,148,652 + -0.4 1,148,653 - -0.1 1,148,653 - +0.1 1,148,653 - -0.7 1,148,653 - -0.2 1,148,653 - +0.8 1,148,653 - -1.0 1,148,653 - +0.1 1,148,656 + -0.8 1,148,656 + -0.2 1,148,656 + -0.7 1,148,656 + +0.2 1,148,656 + +0.5 1,148,656 + +0.8 1,148,656 + +0.4 1,148,656 + +0.4 1,148,656 + +0.8 1,148,656 + +0.4 1,148,656 + -0.2 1,148,656 + -0.0 1,148,656 + -2.0 1,148,656 + +0.4 1,148,656 + +1.1 1,148,656 + -0.3 1,148,656 + +0.4 1,148,656 + +0.1 1,148,657 - -0.1 1,148,657 - -0.1 1,148,657 - -0.5 1,148,657 - -0.6 1,148,657 - -1.6 1,148,657 - +0.4 1,148,657 - +1.0 1,148,657 - -0.9 1,148,657 - +0.8 1,148,657 - +0.4 1,148,657 - -0.7 1,148,657 - -0.5 1,148,657 - -0.5 1,148,657 - +0.0 1,148,657 - -1.0 1,148,657 - -0.0 1,148,657 - +0.1 1,148,657 - -1.3 1,148,657 - +0.4 1,148,658 + -0.8 1,148,658 + +0.2 1,148,658 + -0.8 1,148,658 + -0.3 1,148,658 + +0.8 1,148,658 + +0.5 1,148,658 + +0.2 1,148,658 + +0.1 1,148,658 + -0.5 1,148,658 + -0.7 1,148,658 + -1.1 1,148,659 - -0.7 1,148,659 - -2.6 1,148,659 - +0.4 1,148,659 - +0.2 1,148,659 - -1.2 1,148,659 - +1.6 1,148,659 - -0.3 1,148,659 - +0.9 1,148,845 + Psyr_1015 0.16 +0.8 1,148,845 + Psyr_1015 0.16 +0.5 1,148,845 + Psyr_1015 0.16 +1.8 1,148,846 - Psyr_1015 0.16 -0.6 1,149,058 + Psyr_1015 0.44 -0.2 1,149,058 + Psyr_1015 0.44 +1.3 1,149,058 + Psyr_1015 0.44 -0.1 1,149,058 + Psyr_1015 0.44 +2.8 1,149,058 + Psyr_1015 0.44 -0.9 1,149,058 + Psyr_1015 0.44 +0.2 1,149,059 - Psyr_1015 0.44 +1.1 1,149,059 - Psyr_1015 0.44 -0.5 1,149,142 - Psyr_1015 0.55 +0.4 1,149,274 + Psyr_1015 0.73 +0.8 1,149,274 + Psyr_1015 0.73 +0.1 1,149,274 + Psyr_1015 0.73 +0.8 1,149,274 + Psyr_1015 0.73 -0.0 1,149,274 + Psyr_1015 0.73 -0.3 1,149,274 + Psyr_1015 0.73 -0.7 1,149,275 - Psyr_1015 0.73 +1.0 1,149,275 - Psyr_1015 0.73 +0.6 1,149,275 - Psyr_1015 0.73 -0.5 1,149,275 - Psyr_1015 0.73 +0.2 1,149,352 + Psyr_1015 0.83 -1.1 1,149,352 + Psyr_1015 0.83 +0.2 1,149,353 - Psyr_1015 0.83 +0.3 1,149,353 - Psyr_1015 0.83 +1.9 1,149,353 - Psyr_1015 0.83 +0.4 1,149,397 - Psyr_1015 0.89 +3.1 1,149,451 + +1.8 1,149,649 + -0.0 1,149,651 + +0.2 1,149,651 + -1.7 1,149,651 + -1.0 1,149,652 - -0.4 1,149,652 - +0.7 1,149,652 - -0.3 1,149,652 - -2.4 1,149,652 - +1.1 1,149,652 - +1.8 1,149,662 + +2.2 1,149,662 + -0.0 1,149,662 + -0.2 1,149,662 + -0.4 1,149,663 - -0.9 1,149,663 - +2.0 1,149,663 - -0.8 1,149,665 - -0.2
Or see this region's nucleotide sequence