Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0975 and Psyr_0976 are separated by 85 nucleotides Psyr_0976 and Psyr_0977 are separated by 721 nucleotides
Psyr_0975: Psyr_0975 - homocysteine S-methyltransferase, at 1,107,343 to 1,108,275
_0975
Psyr_0976: Psyr_0976 - Malate:quinone-oxidoreductase, at 1,108,361 to 1,109,869
_0976
Psyr_0977: Psyr_0977 - hypothetical protein, at 1,110,591 to 1,110,824
_0977
Position (kb)
1108
1109
1110 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1107.363 kb on + strand at 1107.363 kb on + strand at 1107.364 kb on - strand at 1107.364 kb on - strand at 1107.365 kb on + strand at 1107.365 kb on + strand at 1107.365 kb on + strand at 1107.366 kb on - strand at 1107.366 kb on - strand at 1107.366 kb on - strand at 1107.366 kb on - strand at 1107.366 kb on - strand at 1107.366 kb on - strand at 1107.378 kb on + strand at 1107.378 kb on + strand at 1107.379 kb on - strand at 1107.379 kb on - strand at 1107.379 kb on - strand at 1107.379 kb on - strand at 1107.379 kb on - strand at 1107.390 kb on + strand at 1107.390 kb on + strand at 1107.390 kb on + strand at 1107.391 kb on - strand at 1107.391 kb on - strand at 1107.391 kb on - strand at 1107.391 kb on - strand at 1107.391 kb on - strand at 1107.398 kb on + strand at 1107.398 kb on + strand at 1107.398 kb on + strand at 1107.399 kb on - strand at 1107.399 kb on - strand at 1107.399 kb on - strand at 1107.399 kb on - strand at 1107.520 kb on + strand, within Psyr_0975 at 1107.520 kb on + strand, within Psyr_0975 at 1107.520 kb on + strand, within Psyr_0975 at 1107.520 kb on + strand, within Psyr_0975 at 1107.520 kb on + strand, within Psyr_0975 at 1107.520 kb on + strand, within Psyr_0975 at 1107.521 kb on - strand, within Psyr_0975 at 1107.522 kb on + strand, within Psyr_0975 at 1107.552 kb on + strand, within Psyr_0975 at 1107.553 kb on - strand, within Psyr_0975 at 1107.560 kb on - strand, within Psyr_0975 at 1107.727 kb on + strand, within Psyr_0975 at 1107.727 kb on + strand, within Psyr_0975 at 1107.728 kb on - strand, within Psyr_0975 at 1107.744 kb on + strand, within Psyr_0975 at 1107.745 kb on - strand, within Psyr_0975 at 1107.784 kb on + strand, within Psyr_0975 at 1107.785 kb on - strand, within Psyr_0975 at 1107.785 kb on - strand, within Psyr_0975 at 1108.256 kb on + strand at 1108.257 kb on - strand at 1108.299 kb on - strand at 1108.299 kb on - strand at 1108.340 kb on + strand at 1108.438 kb on - strand at 1108.637 kb on + strand, within Psyr_0976 at 1108.917 kb on + strand, within Psyr_0976 at 1109.011 kb on - strand, within Psyr_0976 at 1109.171 kb on + strand, within Psyr_0976 at 1109.171 kb on + strand, within Psyr_0976 at 1109.171 kb on + strand, within Psyr_0976 at 1109.171 kb on + strand, within Psyr_0976 at 1109.172 kb on - strand, within Psyr_0976 at 1109.172 kb on - strand, within Psyr_0976 at 1109.172 kb on - strand, within Psyr_0976 at 1109.172 kb on - strand, within Psyr_0976 at 1109.433 kb on + strand, within Psyr_0976 at 1109.434 kb on - strand, within Psyr_0976 at 1109.434 kb on - strand, within Psyr_0976 at 1109.584 kb on - strand, within Psyr_0976 at 1109.673 kb on + strand, within Psyr_0976 at 1109.673 kb on + strand, within Psyr_0976 at 1109.674 kb on - strand, within Psyr_0976 at 1109.796 kb on - strand at 1109.843 kb on + strand at 1109.843 kb on + strand at 1109.843 kb on + strand at 1109.844 kb on - strand at 1109.844 kb on - strand at 1109.910 kb on - strand at 1109.910 kb on - strand at 1110.215 kb on + strand at 1110.215 kb on + strand at 1110.215 kb on + strand at 1110.216 kb on - strand at 1110.216 kb on - strand at 1110.225 kb on + strand at 1110.225 kb on + strand at 1110.225 kb on + strand at 1110.229 kb on + strand at 1110.229 kb on + strand at 1110.229 kb on + strand at 1110.229 kb on + strand at 1110.230 kb on - strand at 1110.459 kb on + strand at 1110.459 kb on + strand at 1110.471 kb on - strand at 1110.473 kb on + strand at 1110.474 kb on - strand at 1110.483 kb on - strand at 1110.517 kb on + strand at 1110.517 kb on + strand at 1110.518 kb on - strand at 1110.518 kb on - strand at 1110.519 kb on + strand at 1110.534 kb on + strand at 1110.534 kb on + strand at 1110.534 kb on + strand at 1110.534 kb on + strand at 1110.534 kb on + strand at 1110.535 kb on - strand at 1110.535 kb on - strand at 1110.535 kb on - strand at 1110.535 kb on - strand at 1110.535 kb on - strand at 1110.536 kb on + strand at 1110.537 kb on - strand at 1110.537 kb on - strand at 1110.567 kb on - strand at 1110.567 kb on - strand at 1110.577 kb on + strand at 1110.578 kb on - strand at 1110.845 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 1,107,363 + -0.5 1,107,363 + -1.1 1,107,364 - +0.7 1,107,364 - -0.6 1,107,365 + -0.3 1,107,365 + -1.0 1,107,365 + -1.0 1,107,366 - +1.3 1,107,366 - +0.1 1,107,366 - +0.0 1,107,366 - -0.4 1,107,366 - +1.3 1,107,366 - +1.0 1,107,378 + -0.9 1,107,378 + -0.3 1,107,379 - -1.3 1,107,379 - +0.4 1,107,379 - -1.1 1,107,379 - +1.6 1,107,379 - -0.2 1,107,390 + +0.3 1,107,390 + -0.0 1,107,390 + +0.2 1,107,391 - +2.1 1,107,391 - -0.6 1,107,391 - +1.1 1,107,391 - +0.1 1,107,391 - -0.3 1,107,398 + +2.4 1,107,398 + -0.8 1,107,398 + -0.5 1,107,399 - +0.1 1,107,399 - +0.6 1,107,399 - +0.9 1,107,399 - -0.1 1,107,520 + Psyr_0975 0.19 -0.2 1,107,520 + Psyr_0975 0.19 -1.2 1,107,520 + Psyr_0975 0.19 -0.0 1,107,520 + Psyr_0975 0.19 +0.4 1,107,520 + Psyr_0975 0.19 +0.6 1,107,520 + Psyr_0975 0.19 -0.5 1,107,521 - Psyr_0975 0.19 +1.2 1,107,522 + Psyr_0975 0.19 -0.2 1,107,552 + Psyr_0975 0.22 +0.9 1,107,553 - Psyr_0975 0.23 -0.4 1,107,560 - Psyr_0975 0.23 +0.2 1,107,727 + Psyr_0975 0.41 +0.1 1,107,727 + Psyr_0975 0.41 +0.0 1,107,728 - Psyr_0975 0.41 +2.3 1,107,744 + Psyr_0975 0.43 +0.6 1,107,745 - Psyr_0975 0.43 +0.8 1,107,784 + Psyr_0975 0.47 +0.6 1,107,785 - Psyr_0975 0.47 -2.4 1,107,785 - Psyr_0975 0.47 -0.1 1,108,256 + +0.9 1,108,257 - +0.4 1,108,299 - +0.3 1,108,299 - -0.1 1,108,340 + +0.5 1,108,438 - +0.0 1,108,637 + Psyr_0976 0.18 +0.7 1,108,917 + Psyr_0976 0.37 +0.2 1,109,011 - Psyr_0976 0.43 +3.6 1,109,171 + Psyr_0976 0.54 -0.6 1,109,171 + Psyr_0976 0.54 -3.0 1,109,171 + Psyr_0976 0.54 -1.6 1,109,171 + Psyr_0976 0.54 -1.1 1,109,172 - Psyr_0976 0.54 -1.1 1,109,172 - Psyr_0976 0.54 +0.7 1,109,172 - Psyr_0976 0.54 -0.3 1,109,172 - Psyr_0976 0.54 -2.3 1,109,433 + Psyr_0976 0.71 -0.5 1,109,434 - Psyr_0976 0.71 -1.1 1,109,434 - Psyr_0976 0.71 -2.2 1,109,584 - Psyr_0976 0.81 -1.3 1,109,673 + Psyr_0976 0.87 -2.6 1,109,673 + Psyr_0976 0.87 -1.0 1,109,674 - Psyr_0976 0.87 +0.1 1,109,796 - -0.3 1,109,843 + -0.1 1,109,843 + +0.7 1,109,843 + -3.3 1,109,844 - -1.6 1,109,844 - -2.4 1,109,910 - +0.0 1,109,910 - -0.7 1,110,215 + +0.1 1,110,215 + -0.8 1,110,215 + -0.8 1,110,216 - -1.9 1,110,216 - +0.1 1,110,225 + -3.2 1,110,225 + -0.8 1,110,225 + -0.0 1,110,229 + +0.4 1,110,229 + +0.2 1,110,229 + -0.3 1,110,229 + +0.5 1,110,230 - +0.5 1,110,459 + -0.5 1,110,459 + +0.5 1,110,471 - +0.5 1,110,473 + -0.2 1,110,474 - -0.3 1,110,483 - +0.4 1,110,517 + -0.5 1,110,517 + +1.3 1,110,518 - -0.6 1,110,518 - -0.8 1,110,519 + +0.1 1,110,534 + -1.0 1,110,534 + +0.9 1,110,534 + +0.2 1,110,534 + -0.4 1,110,534 + -0.4 1,110,535 - -0.3 1,110,535 - +0.5 1,110,535 - +0.5 1,110,535 - +0.1 1,110,535 - +0.1 1,110,536 + +0.0 1,110,537 - +3.3 1,110,537 - +0.7 1,110,567 - +0.9 1,110,567 - -1.8 1,110,577 + -0.3 1,110,578 - -2.3 1,110,845 - +0.2
Or see this region's nucleotide sequence