Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0607 and Psyr_0608 are separated by 79 nucleotides Psyr_0608 and Psyr_0609 are separated by 202 nucleotides
Psyr_0607: Psyr_0607 - cystathionine beta-lyase, at 696,911 to 698,077
_0607
Psyr_0608: Psyr_0608 - Protein of unknown function DUF1016, at 698,157 to 699,173
_0608
Psyr_0609: Psyr_0609 - Glutathione S-transferase, C-terminal, at 699,376 to 700,218
_0609
Position (kb) 698
699
700 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 697.206 kb on + strand, within Psyr_0607 at 697.206 kb on + strand, within Psyr_0607 at 697.320 kb on + strand, within Psyr_0607 at 697.320 kb on + strand, within Psyr_0607 at 697.343 kb on - strand, within Psyr_0607 at 697.343 kb on - strand, within Psyr_0607 at 697.375 kb on + strand, within Psyr_0607 at 697.437 kb on + strand, within Psyr_0607 at 697.437 kb on + strand, within Psyr_0607 at 697.438 kb on - strand, within Psyr_0607 at 697.438 kb on - strand, within Psyr_0607 at 697.438 kb on - strand, within Psyr_0607 at 697.438 kb on - strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.489 kb on - strand, within Psyr_0607 at 697.489 kb on - strand, within Psyr_0607 at 697.489 kb on - strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.649 kb on - strand, within Psyr_0607 at 697.665 kb on + strand, within Psyr_0607 at 697.666 kb on - strand, within Psyr_0607 at 697.728 kb on + strand, within Psyr_0607 at 697.728 kb on + strand, within Psyr_0607 at 697.728 kb on + strand, within Psyr_0607 at 697.729 kb on - strand, within Psyr_0607 at 697.894 kb on - strand, within Psyr_0607 at 697.969 kb on + strand at 697.969 kb on + strand at 697.969 kb on + strand at 698.132 kb on + strand at 698.132 kb on + strand at 698.133 kb on - strand at 698.133 kb on - strand at 698.155 kb on + strand at 698.155 kb on + strand at 698.155 kb on + strand at 698.185 kb on + strand at 698.211 kb on + strand at 698.212 kb on - strand at 698.212 kb on - strand at 698.281 kb on + strand, within Psyr_0608 at 698.282 kb on - strand, within Psyr_0608 at 698.317 kb on - strand, within Psyr_0608 at 698.317 kb on - strand, within Psyr_0608 at 698.317 kb on - strand, within Psyr_0608 at 698.317 kb on - strand, within Psyr_0608 at 698.317 kb on - strand, within Psyr_0608 at 698.377 kb on + strand, within Psyr_0608 at 698.377 kb on + strand, within Psyr_0608 at 698.377 kb on + strand, within Psyr_0608 at 698.399 kb on - strand, within Psyr_0608 at 698.541 kb on + strand, within Psyr_0608 at 698.542 kb on - strand, within Psyr_0608 at 698.633 kb on + strand, within Psyr_0608 at 698.633 kb on + strand, within Psyr_0608 at 698.634 kb on - strand, within Psyr_0608 at 698.634 kb on - strand, within Psyr_0608 at 698.634 kb on - strand, within Psyr_0608 at 698.634 kb on - strand, within Psyr_0608 at 698.634 kb on - strand, within Psyr_0608 at 698.634 kb on - strand, within Psyr_0608 at 698.635 kb on + strand, within Psyr_0608 at 698.635 kb on + strand, within Psyr_0608 at 698.635 kb on + strand, within Psyr_0608 at 698.635 kb on + strand, within Psyr_0608 at 698.635 kb on + strand, within Psyr_0608 at 698.635 kb on + strand, within Psyr_0608 at 698.635 kb on + strand, within Psyr_0608 at 698.635 kb on + strand, within Psyr_0608 at 698.635 kb on + strand, within Psyr_0608 at 698.635 kb on + strand, within Psyr_0608 at 698.635 kb on + strand, within Psyr_0608 at 698.635 kb on + strand, within Psyr_0608 at 698.635 kb on + strand, within Psyr_0608 at 698.636 kb on - strand, within Psyr_0608 at 698.636 kb on - strand, within Psyr_0608 at 698.636 kb on - strand, within Psyr_0608 at 698.636 kb on - strand, within Psyr_0608 at 698.636 kb on - strand, within Psyr_0608 at 698.636 kb on - strand, within Psyr_0608 at 698.636 kb on - strand, within Psyr_0608 at 698.649 kb on + strand, within Psyr_0608 at 698.649 kb on + strand, within Psyr_0608 at 698.649 kb on + strand, within Psyr_0608 at 698.649 kb on + strand, within Psyr_0608 at 698.649 kb on + strand, within Psyr_0608 at 698.649 kb on + strand, within Psyr_0608 at 698.649 kb on + strand, within Psyr_0608 at 698.650 kb on - strand, within Psyr_0608 at 698.650 kb on - strand, within Psyr_0608 at 698.650 kb on - strand, within Psyr_0608 at 698.650 kb on - strand, within Psyr_0608 at 698.650 kb on - strand, within Psyr_0608 at 698.651 kb on + strand, within Psyr_0608 at 698.651 kb on + strand, within Psyr_0608 at 698.652 kb on - strand, within Psyr_0608 at 698.652 kb on - strand, within Psyr_0608 at 698.652 kb on - strand, within Psyr_0608 at 698.724 kb on + strand, within Psyr_0608 at 698.840 kb on + strand, within Psyr_0608 at 698.840 kb on + strand, within Psyr_0608 at 698.840 kb on + strand, within Psyr_0608 at 698.840 kb on + strand, within Psyr_0608 at 698.840 kb on + strand, within Psyr_0608 at 698.840 kb on + strand, within Psyr_0608 at 698.841 kb on - strand, within Psyr_0608 at 698.841 kb on - strand, within Psyr_0608 at 698.853 kb on + strand, within Psyr_0608 at 698.854 kb on - strand, within Psyr_0608 at 698.854 kb on - strand, within Psyr_0608 at 698.894 kb on - strand, within Psyr_0608 at 698.903 kb on - strand, within Psyr_0608 at 699.034 kb on + strand, within Psyr_0608 at 699.034 kb on + strand, within Psyr_0608 at 699.034 kb on + strand, within Psyr_0608 at 699.035 kb on - strand, within Psyr_0608 at 699.035 kb on - strand, within Psyr_0608 at 699.035 kb on - strand, within Psyr_0608 at 699.035 kb on - strand, within Psyr_0608 at 699.053 kb on - strand, within Psyr_0608 at 699.141 kb on + strand at 699.141 kb on + strand at 699.141 kb on + strand at 699.141 kb on + strand at 699.141 kb on + strand at 699.141 kb on + strand at 699.142 kb on - strand at 699.142 kb on - strand at 699.142 kb on - strand at 699.142 kb on - strand at 699.142 kb on - strand at 699.142 kb on - strand at 699.167 kb on + strand at 699.168 kb on - strand at 699.174 kb on - strand at 699.182 kb on + strand at 699.182 kb on + strand at 699.182 kb on + strand at 699.183 kb on - strand at 699.183 kb on - strand at 699.251 kb on - strand at 699.290 kb on + strand at 699.290 kb on + strand at 699.290 kb on + strand at 699.290 kb on + strand at 699.290 kb on + strand at 699.290 kb on + strand at 699.290 kb on + strand at 699.290 kb on + strand at 699.291 kb on - strand at 699.291 kb on - strand at 699.291 kb on - strand at 699.291 kb on - strand at 699.291 kb on - strand at 699.291 kb on - strand at 699.336 kb on + strand at 699.336 kb on + strand at 699.336 kb on + strand at 699.336 kb on + strand at 699.336 kb on + strand at 699.337 kb on - strand at 699.337 kb on - strand at 699.337 kb on - strand at 699.337 kb on - strand at 699.337 kb on - strand at 699.377 kb on + strand at 699.377 kb on + strand at 699.377 kb on + strand at 699.377 kb on + strand at 699.377 kb on + strand at 699.377 kb on + strand at 699.377 kb on + strand at 699.377 kb on + strand at 699.377 kb on + strand at 699.377 kb on + strand at 699.378 kb on - strand at 699.378 kb on - strand at 699.378 kb on - strand at 699.378 kb on - strand at 699.378 kb on - strand at 699.378 kb on - strand at 699.378 kb on - strand at 699.378 kb on - strand at 699.378 kb on - strand at 699.378 kb on - strand at 699.378 kb on - strand at 699.378 kb on - strand at 699.545 kb on + strand, within Psyr_0609 at 699.546 kb on - strand, within Psyr_0609 at 699.546 kb on - strand, within Psyr_0609 at 699.572 kb on + strand, within Psyr_0609 at 699.605 kb on + strand, within Psyr_0609 at 699.605 kb on + strand, within Psyr_0609 at 699.605 kb on + strand, within Psyr_0609 at 699.606 kb on - strand, within Psyr_0609 at 699.606 kb on - strand, within Psyr_0609 at 699.606 kb on - strand, within Psyr_0609 at 699.698 kb on + strand, within Psyr_0609 at 699.719 kb on + strand, within Psyr_0609 at 699.720 kb on - strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.725 kb on + strand, within Psyr_0609 at 699.726 kb on - strand, within Psyr_0609 at 699.752 kb on + strand, within Psyr_0609 at 699.848 kb on + strand, within Psyr_0609 at 699.848 kb on + strand, within Psyr_0609 at 699.858 kb on + strand, within Psyr_0609 at 699.975 kb on + strand, within Psyr_0609 at 699.975 kb on + strand, within Psyr_0609 at 699.976 kb on - strand, within Psyr_0609 at 699.976 kb on - strand, within Psyr_0609 at 699.995 kb on + strand, within Psyr_0609 at 699.995 kb on + strand, within Psyr_0609 at 700.076 kb on + strand, within Psyr_0609 at 700.076 kb on + strand, within Psyr_0609 at 700.077 kb on - strand, within Psyr_0609 at 700.077 kb on - strand, within Psyr_0609 at 700.077 kb on - strand, within Psyr_0609
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 697,206 + Psyr_0607 0.25 +0.8 697,206 + Psyr_0607 0.25 -0.5 697,320 + Psyr_0607 0.35 +0.4 697,320 + Psyr_0607 0.35 -1.2 697,343 - Psyr_0607 0.37 +0.5 697,343 - Psyr_0607 0.37 +0.8 697,375 + Psyr_0607 0.40 +0.4 697,437 + Psyr_0607 0.45 -0.8 697,437 + Psyr_0607 0.45 -0.8 697,438 - Psyr_0607 0.45 +1.0 697,438 - Psyr_0607 0.45 +0.5 697,438 - Psyr_0607 0.45 +2.2 697,438 - Psyr_0607 0.45 -0.8 697,488 + Psyr_0607 0.49 +0.3 697,488 + Psyr_0607 0.49 -0.2 697,488 + Psyr_0607 0.49 -0.4 697,488 + Psyr_0607 0.49 +1.3 697,488 + Psyr_0607 0.49 +2.4 697,488 + Psyr_0607 0.49 -0.1 697,488 + Psyr_0607 0.49 +2.4 697,489 - Psyr_0607 0.50 +1.4 697,489 - Psyr_0607 0.50 +0.2 697,489 - Psyr_0607 0.50 +3.2 697,608 + Psyr_0607 0.60 -0.8 697,608 + Psyr_0607 0.60 +2.4 697,608 + Psyr_0607 0.60 +0.6 697,608 + Psyr_0607 0.60 -0.1 697,608 + Psyr_0607 0.60 +0.1 697,609 - Psyr_0607 0.60 -1.0 697,609 - Psyr_0607 0.60 -0.0 697,609 - Psyr_0607 0.60 +0.2 697,609 - Psyr_0607 0.60 -1.0 697,609 - Psyr_0607 0.60 +0.2 697,609 - Psyr_0607 0.60 +0.6 697,649 - Psyr_0607 0.63 -0.6 697,665 + Psyr_0607 0.65 +0.8 697,666 - Psyr_0607 0.65 +0.6 697,728 + Psyr_0607 0.70 -0.9 697,728 + Psyr_0607 0.70 +1.6 697,728 + Psyr_0607 0.70 -0.9 697,729 - Psyr_0607 0.70 -0.2 697,894 - Psyr_0607 0.84 -0.8 697,969 + +0.4 697,969 + -1.2 697,969 + +0.8 698,132 + -0.0 698,132 + -0.1 698,133 - +3.3 698,133 - +0.4 698,155 + +0.8 698,155 + -1.4 698,155 + +0.0 698,185 + -0.8 698,211 + -2.0 698,212 - +0.1 698,212 - -1.3 698,281 + Psyr_0608 0.12 +0.1 698,282 - Psyr_0608 0.12 -0.5 698,317 - Psyr_0608 0.16 -0.2 698,317 - Psyr_0608 0.16 +0.3 698,317 - Psyr_0608 0.16 +0.5 698,317 - Psyr_0608 0.16 +1.2 698,317 - Psyr_0608 0.16 +1.4 698,377 + Psyr_0608 0.22 +1.1 698,377 + Psyr_0608 0.22 +1.4 698,377 + Psyr_0608 0.22 +0.6 698,399 - Psyr_0608 0.24 -0.7 698,541 + Psyr_0608 0.38 -1.2 698,542 - Psyr_0608 0.38 +0.7 698,633 + Psyr_0608 0.47 -0.2 698,633 + Psyr_0608 0.47 +0.4 698,634 - Psyr_0608 0.47 -0.1 698,634 - Psyr_0608 0.47 -0.5 698,634 - Psyr_0608 0.47 -0.8 698,634 - Psyr_0608 0.47 +1.1 698,634 - Psyr_0608 0.47 -0.2 698,634 - Psyr_0608 0.47 -0.5 698,635 + Psyr_0608 0.47 +1.3 698,635 + Psyr_0608 0.47 +1.0 698,635 + Psyr_0608 0.47 +0.4 698,635 + Psyr_0608 0.47 +0.2 698,635 + Psyr_0608 0.47 +0.9 698,635 + Psyr_0608 0.47 -0.8 698,635 + Psyr_0608 0.47 +0.5 698,635 + Psyr_0608 0.47 +1.1 698,635 + Psyr_0608 0.47 +0.6 698,635 + Psyr_0608 0.47 +0.9 698,635 + Psyr_0608 0.47 -0.1 698,635 + Psyr_0608 0.47 -0.8 698,635 + Psyr_0608 0.47 -0.1 698,636 - Psyr_0608 0.47 +0.6 698,636 - Psyr_0608 0.47 -0.8 698,636 - Psyr_0608 0.47 +0.7 698,636 - Psyr_0608 0.47 +1.0 698,636 - Psyr_0608 0.47 +0.2 698,636 - Psyr_0608 0.47 +0.2 698,636 - Psyr_0608 0.47 -0.1 698,649 + Psyr_0608 0.48 +0.1 698,649 + Psyr_0608 0.48 +1.0 698,649 + Psyr_0608 0.48 -0.9 698,649 + Psyr_0608 0.48 -0.9 698,649 + Psyr_0608 0.48 +0.6 698,649 + Psyr_0608 0.48 +1.4 698,649 + Psyr_0608 0.48 -0.1 698,650 - Psyr_0608 0.48 -0.9 698,650 - Psyr_0608 0.48 +0.2 698,650 - Psyr_0608 0.48 +0.7 698,650 - Psyr_0608 0.48 -0.3 698,650 - Psyr_0608 0.48 +0.4 698,651 + Psyr_0608 0.49 -0.4 698,651 + Psyr_0608 0.49 +3.4 698,652 - Psyr_0608 0.49 -0.3 698,652 - Psyr_0608 0.49 -1.5 698,652 - Psyr_0608 0.49 -1.0 698,724 + Psyr_0608 0.56 +2.5 698,840 + Psyr_0608 0.67 +0.9 698,840 + Psyr_0608 0.67 +0.7 698,840 + Psyr_0608 0.67 +1.9 698,840 + Psyr_0608 0.67 -0.2 698,840 + Psyr_0608 0.67 +0.8 698,840 + Psyr_0608 0.67 -0.1 698,841 - Psyr_0608 0.67 +0.8 698,841 - Psyr_0608 0.67 +0.8 698,853 + Psyr_0608 0.68 +0.6 698,854 - Psyr_0608 0.69 +0.5 698,854 - Psyr_0608 0.69 -0.4 698,894 - Psyr_0608 0.72 -2.3 698,903 - Psyr_0608 0.73 -0.2 699,034 + Psyr_0608 0.86 +0.4 699,034 + Psyr_0608 0.86 -0.2 699,034 + Psyr_0608 0.86 +0.7 699,035 - Psyr_0608 0.86 +1.5 699,035 - Psyr_0608 0.86 +0.3 699,035 - Psyr_0608 0.86 +0.1 699,035 - Psyr_0608 0.86 +0.4 699,053 - Psyr_0608 0.88 +0.4 699,141 + +0.4 699,141 + +0.2 699,141 + -1.2 699,141 + -0.1 699,141 + -1.5 699,141 + +0.1 699,142 - +0.4 699,142 - +1.7 699,142 - -0.8 699,142 - +3.0 699,142 - +0.1 699,142 - -0.7 699,167 + -3.0 699,168 - -2.2 699,174 - +0.6 699,182 + +1.8 699,182 + -0.3 699,182 + -1.1 699,183 - +0.1 699,183 - +2.6 699,251 - -0.8 699,290 + +0.3 699,290 + -0.6 699,290 + +0.8 699,290 + -1.3 699,290 + +0.3 699,290 + -1.1 699,290 + +0.4 699,290 + -0.7 699,291 - +3.0 699,291 - +0.5 699,291 - -0.4 699,291 - +0.5 699,291 - -0.4 699,291 - -0.1 699,336 + +0.3 699,336 + -0.1 699,336 + -0.6 699,336 + +1.0 699,336 + -0.0 699,337 - -0.1 699,337 - +0.8 699,337 - -0.6 699,337 - +0.6 699,337 - -0.2 699,377 + -0.3 699,377 + +0.8 699,377 + -0.5 699,377 + +1.4 699,377 + -0.4 699,377 + +1.0 699,377 + +1.2 699,377 + +0.9 699,377 + +0.8 699,377 + +1.0 699,378 - -0.8 699,378 - -0.1 699,378 - +1.8 699,378 - -0.1 699,378 - +0.7 699,378 - +0.9 699,378 - -0.1 699,378 - +1.0 699,378 - -0.5 699,378 - -0.3 699,378 - +0.8 699,378 - +0.6 699,545 + Psyr_0609 0.20 +0.4 699,546 - Psyr_0609 0.20 -0.1 699,546 - Psyr_0609 0.20 -0.6 699,572 + Psyr_0609 0.23 +0.3 699,605 + Psyr_0609 0.27 -0.4 699,605 + Psyr_0609 0.27 -0.8 699,605 + Psyr_0609 0.27 +1.5 699,606 - Psyr_0609 0.27 +0.6 699,606 - Psyr_0609 0.27 -0.6 699,606 - Psyr_0609 0.27 +0.6 699,698 + Psyr_0609 0.38 +0.6 699,719 + Psyr_0609 0.41 +0.6 699,720 - Psyr_0609 0.41 +0.6 699,725 + Psyr_0609 0.41 +0.7 699,725 + Psyr_0609 0.41 +0.8 699,725 + Psyr_0609 0.41 -0.6 699,725 + Psyr_0609 0.41 -1.2 699,725 + Psyr_0609 0.41 +1.8 699,725 + Psyr_0609 0.41 +0.3 699,725 + Psyr_0609 0.41 -0.3 699,725 + Psyr_0609 0.41 +1.5 699,726 - Psyr_0609 0.42 -0.9 699,752 + Psyr_0609 0.45 -0.2 699,848 + Psyr_0609 0.56 +1.4 699,848 + Psyr_0609 0.56 -0.3 699,858 + Psyr_0609 0.57 +0.9 699,975 + Psyr_0609 0.71 +0.1 699,975 + Psyr_0609 0.71 -0.3 699,976 - Psyr_0609 0.71 +1.6 699,976 - Psyr_0609 0.71 +0.1 699,995 + Psyr_0609 0.73 +1.3 699,995 + Psyr_0609 0.73 +0.7 700,076 + Psyr_0609 0.83 -0.6 700,076 + Psyr_0609 0.83 +0.3 700,077 - Psyr_0609 0.83 +0.7 700,077 - Psyr_0609 0.83 -2.2 700,077 - Psyr_0609 0.83 +1.4
Or see this region's nucleotide sequence