Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0440 and Psyr_0441 are separated by 692 nucleotides Psyr_0441 and Psyr_0442 are separated by 406 nucleotides
Psyr_0440: Psyr_0440 - Cobyrinic acid a,c-diamide synthase, at 477,339 to 478,118
_0440
Psyr_0441: Psyr_0441 - conserved hypothetical protein, at 478,811 to 479,206
_0441
Psyr_0442: Psyr_0442 - Malonate decarboxylase, alpha subunit, at 479,613 to 481,289
_0442
Position (kb) 478
479
480 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 477.829 kb on - strand, within Psyr_0440 at 477.984 kb on + strand, within Psyr_0440 at 477.984 kb on + strand, within Psyr_0440 at 478.144 kb on + strand at 478.145 kb on - strand at 478.303 kb on - strand at 478.303 kb on - strand at 478.303 kb on - strand at 478.332 kb on - strand at 478.332 kb on - strand at 478.332 kb on - strand at 478.363 kb on + strand at 478.363 kb on + strand at 478.492 kb on + strand at 478.492 kb on + strand at 478.492 kb on + strand at 478.493 kb on - strand at 478.493 kb on - strand at 478.493 kb on - strand at 478.493 kb on - strand at 478.493 kb on - strand at 478.493 kb on - strand at 478.493 kb on - strand at 478.493 kb on - strand at 478.494 kb on + strand at 478.494 kb on + strand at 478.494 kb on + strand at 478.494 kb on + strand at 478.495 kb on - strand at 478.495 kb on - strand at 478.495 kb on - strand at 478.743 kb on + strand at 478.743 kb on + strand at 478.743 kb on + strand at 478.743 kb on + strand at 478.743 kb on + strand at 478.812 kb on + strand at 478.829 kb on + strand at 478.829 kb on + strand at 478.829 kb on + strand at 478.829 kb on + strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.969 kb on + strand, within Psyr_0441 at 478.970 kb on - strand, within Psyr_0441 at 479.041 kb on - strand, within Psyr_0441 at 479.041 kb on - strand, within Psyr_0441 at 479.041 kb on - strand, within Psyr_0441 at 479.156 kb on + strand, within Psyr_0441 at 479.196 kb on - strand at 479.196 kb on - strand at 479.197 kb on + strand at 479.197 kb on + strand at 479.197 kb on + strand at 479.197 kb on + strand at 479.197 kb on + strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.255 kb on - strand at 479.283 kb on + strand at 479.296 kb on + strand at 479.305 kb on - strand at 479.370 kb on - strand at 479.376 kb on - strand at 479.464 kb on - strand at 479.577 kb on + strand at 479.578 kb on - strand at 479.578 kb on - strand at 479.686 kb on + strand at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.024 kb on + strand, within Psyr_0442 at 480.025 kb on - strand, within Psyr_0442 at 480.102 kb on + strand, within Psyr_0442 at 480.102 kb on + strand, within Psyr_0442 at 480.102 kb on + strand, within Psyr_0442 at 480.102 kb on + strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 477,829 - Psyr_0440 0.63 -0.3 477,984 + Psyr_0440 0.83 -0.5 477,984 + Psyr_0440 0.83 +0.8 478,144 + -0.5 478,145 - +1.4 478,303 - +0.2 478,303 - +2.2 478,303 - -0.2 478,332 - +0.3 478,332 - -0.3 478,332 - -0.3 478,363 + -1.2 478,363 + -0.1 478,492 + +2.6 478,492 + +0.1 478,492 + -0.5 478,493 - +0.2 478,493 - +0.9 478,493 - -0.4 478,493 - -0.5 478,493 - +0.9 478,493 - +0.5 478,493 - -0.3 478,493 - -0.9 478,494 + +2.1 478,494 + -0.3 478,494 + +0.1 478,494 + -1.4 478,495 - -0.4 478,495 - -0.9 478,495 - +0.5 478,743 + +0.4 478,743 + -1.8 478,743 + -0.6 478,743 + -0.5 478,743 + +0.9 478,812 + +0.4 478,829 + +1.1 478,829 + +0.6 478,829 + -0.0 478,829 + +1.1 478,830 - -0.9 478,830 - +1.6 478,830 - -0.5 478,830 - +1.3 478,830 - -0.9 478,830 - -0.4 478,830 - +0.1 478,830 - -0.3 478,830 - +0.6 478,830 - +0.3 478,969 + Psyr_0441 0.40 +0.5 478,970 - Psyr_0441 0.40 +1.1 479,041 - Psyr_0441 0.58 +0.8 479,041 - Psyr_0441 0.58 -0.1 479,041 - Psyr_0441 0.58 -0.1 479,156 + Psyr_0441 0.87 +0.4 479,196 - +0.7 479,196 - +0.8 479,197 + -1.0 479,197 + -0.2 479,197 + -0.9 479,197 + +0.6 479,197 + +1.3 479,198 - -1.9 479,198 - -0.5 479,198 - -1.3 479,198 - -0.1 479,198 - -3.2 479,198 - +1.1 479,198 - -0.6 479,198 - +1.2 479,198 - -0.0 479,198 - -1.4 479,198 - -0.2 479,198 - +0.1 479,255 - -0.6 479,283 + -0.9 479,296 + -0.5 479,305 - +0.2 479,370 - +0.1 479,376 - +0.1 479,464 - -0.4 479,577 + +0.4 479,578 - +1.3 479,578 - +0.8 479,686 + -0.4 480,012 + Psyr_0442 0.24 +1.6 480,012 + Psyr_0442 0.24 +2.1 480,012 + Psyr_0442 0.24 +1.1 480,012 + Psyr_0442 0.24 +0.5 480,012 + Psyr_0442 0.24 -0.3 480,012 + Psyr_0442 0.24 +0.1 480,013 - Psyr_0442 0.24 -0.8 480,013 - Psyr_0442 0.24 +0.1 480,013 - Psyr_0442 0.24 -1.8 480,013 - Psyr_0442 0.24 +0.9 480,013 - Psyr_0442 0.24 -0.5 480,024 + Psyr_0442 0.25 -1.3 480,025 - Psyr_0442 0.25 +0.8 480,102 + Psyr_0442 0.29 -0.4 480,102 + Psyr_0442 0.29 -0.1 480,102 + Psyr_0442 0.29 -0.2 480,102 + Psyr_0442 0.29 +0.5 480,103 - Psyr_0442 0.29 +1.1 480,103 - Psyr_0442 0.29 +0.7 480,103 - Psyr_0442 0.29 -0.1 480,103 - Psyr_0442 0.29 +0.2 480,103 - Psyr_0442 0.29 +0.7 480,103 - Psyr_0442 0.29 -0.3
Or see this region's nucleotide sequence