Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0437 and Psyr_0438 overlap by 26 nucleotides Psyr_0438 and Psyr_0439 overlap by 25 nucleotides Psyr_0439 and Psyr_0440 are separated by 309 nucleotides
Psyr_0437: Psyr_0437 - acyl carrier protein, putative, at 475,287 to 475,547
_0437
Psyr_0438: Psyr_0438 - Phospholipid/glycerol acyltransferase, at 475,522 to 476,334
_0438
Psyr_0439: Psyr_0439 - conserved hypothetical protein, at 476,310 to 477,029
_0439
Psyr_0440: Psyr_0440 - Cobyrinic acid a,c-diamide synthase, at 477,339 to 478,118
_0440
Position (kb)
476
477
478 Strain fitness (log2 ratio)
-1
0
1
2 at 475.460 kb on + strand, within Psyr_0437 at 475.461 kb on - strand, within Psyr_0437 at 475.523 kb on + strand at 475.523 kb on + strand at 475.523 kb on + strand at 475.523 kb on + strand at 475.523 kb on + strand at 475.523 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.572 kb on - strand at 475.572 kb on - strand at 475.584 kb on - strand at 475.584 kb on - strand at 475.645 kb on + strand, within Psyr_0438 at 475.645 kb on + strand, within Psyr_0438 at 475.645 kb on + strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.919 kb on + strand, within Psyr_0438 at 475.920 kb on - strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.094 kb on - strand, within Psyr_0438 at 476.094 kb on - strand, within Psyr_0438 at 476.094 kb on - strand, within Psyr_0438 at 476.094 kb on - strand, within Psyr_0438 at 476.288 kb on + strand at 476.288 kb on + strand at 476.289 kb on - strand at 476.289 kb on - strand at 476.291 kb on + strand at 476.291 kb on + strand at 476.527 kb on + strand, within Psyr_0439 at 476.527 kb on + strand, within Psyr_0439 at 476.566 kb on - strand, within Psyr_0439 at 476.566 kb on - strand, within Psyr_0439 at 476.566 kb on - strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.615 kb on - strand, within Psyr_0439 at 476.615 kb on - strand, within Psyr_0439 at 477.066 kb on + strand at 477.067 kb on - strand at 477.141 kb on + strand at 477.141 kb on + strand at 477.141 kb on + strand at 477.142 kb on - strand at 477.145 kb on - strand at 477.160 kb on + strand at 477.160 kb on + strand at 477.161 kb on - strand at 477.181 kb on + strand at 477.181 kb on + strand at 477.181 kb on + strand at 477.181 kb on + strand at 477.182 kb on - strand at 477.182 kb on - strand at 477.256 kb on + strand at 477.257 kb on - strand at 477.267 kb on + strand at 477.268 kb on - strand at 477.268 kb on - strand at 477.437 kb on - strand, within Psyr_0440 at 477.474 kb on + strand, within Psyr_0440 at 477.576 kb on - strand, within Psyr_0440 at 477.579 kb on + strand, within Psyr_0440 at 477.579 kb on + strand, within Psyr_0440 at 477.579 kb on + strand, within Psyr_0440 at 477.579 kb on + strand, within Psyr_0440 at 477.580 kb on - strand, within Psyr_0440 at 477.580 kb on - strand, within Psyr_0440 at 477.580 kb on - strand, within Psyr_0440 at 477.580 kb on - strand, within Psyr_0440 at 477.580 kb on - strand, within Psyr_0440 at 477.716 kb on + strand, within Psyr_0440 at 477.717 kb on - strand, within Psyr_0440 at 477.829 kb on - strand, within Psyr_0440 at 477.984 kb on + strand, within Psyr_0440 at 477.984 kb on + strand, within Psyr_0440
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 475,460 + Psyr_0437 0.66 +0.9 475,461 - Psyr_0437 0.67 +0.2 475,523 + +0.2 475,523 + +0.1 475,523 + +0.3 475,523 + +0.5 475,523 + +0.4 475,523 + +0.5 475,571 + -0.5 475,571 + -1.1 475,571 + -0.1 475,571 + +0.4 475,571 + +0.8 475,571 + -0.0 475,571 + -0.5 475,571 + +0.5 475,572 - +1.9 475,572 - +0.6 475,584 - +0.7 475,584 - -0.4 475,645 + Psyr_0438 0.15 -0.3 475,645 + Psyr_0438 0.15 +0.1 475,645 + Psyr_0438 0.15 -0.5 475,646 - Psyr_0438 0.15 -1.2 475,646 - Psyr_0438 0.15 +1.7 475,646 - Psyr_0438 0.15 +0.9 475,646 - Psyr_0438 0.15 -0.9 475,646 - Psyr_0438 0.15 -0.2 475,646 - Psyr_0438 0.15 +0.6 475,919 + Psyr_0438 0.49 -0.1 475,920 - Psyr_0438 0.49 +0.1 476,093 + Psyr_0438 0.70 -0.5 476,093 + Psyr_0438 0.70 +0.6 476,093 + Psyr_0438 0.70 +0.2 476,093 + Psyr_0438 0.70 -0.1 476,093 + Psyr_0438 0.70 -0.9 476,093 + Psyr_0438 0.70 +0.6 476,093 + Psyr_0438 0.70 +0.3 476,093 + Psyr_0438 0.70 +0.3 476,093 + Psyr_0438 0.70 +0.9 476,093 + Psyr_0438 0.70 -0.5 476,094 - Psyr_0438 0.70 -0.9 476,094 - Psyr_0438 0.70 -1.1 476,094 - Psyr_0438 0.70 +1.5 476,094 - Psyr_0438 0.70 -0.4 476,288 + -0.3 476,288 + -1.0 476,289 - +0.2 476,289 - -0.4 476,291 + -0.6 476,291 + -0.1 476,527 + Psyr_0439 0.30 -1.5 476,527 + Psyr_0439 0.30 -0.8 476,566 - Psyr_0439 0.36 +1.4 476,566 - Psyr_0439 0.36 -0.6 476,566 - Psyr_0439 0.36 -0.4 476,614 + Psyr_0439 0.42 -1.0 476,614 + Psyr_0439 0.42 -1.4 476,614 + Psyr_0439 0.42 +0.2 476,614 + Psyr_0439 0.42 +0.5 476,614 + Psyr_0439 0.42 -0.9 476,615 - Psyr_0439 0.42 -0.2 476,615 - Psyr_0439 0.42 -0.3 477,066 + +0.4 477,067 - +0.4 477,141 + -0.0 477,141 + -0.0 477,141 + +0.1 477,142 - -1.2 477,145 - +0.7 477,160 + -0.5 477,160 + +0.8 477,161 - -1.1 477,181 + -0.5 477,181 + -0.0 477,181 + -0.4 477,181 + +0.0 477,182 - +0.6 477,182 - +0.3 477,256 + +0.7 477,257 - -0.7 477,267 + +0.5 477,268 - +0.1 477,268 - -0.2 477,437 - Psyr_0440 0.13 -0.2 477,474 + Psyr_0440 0.17 -0.2 477,576 - Psyr_0440 0.30 +0.1 477,579 + Psyr_0440 0.31 -1.2 477,579 + Psyr_0440 0.31 -0.5 477,579 + Psyr_0440 0.31 +0.5 477,579 + Psyr_0440 0.31 +0.1 477,580 - Psyr_0440 0.31 +1.1 477,580 - Psyr_0440 0.31 +0.1 477,580 - Psyr_0440 0.31 -0.7 477,580 - Psyr_0440 0.31 +0.2 477,580 - Psyr_0440 0.31 +0.1 477,716 + Psyr_0440 0.48 -0.6 477,717 - Psyr_0440 0.48 -0.5 477,829 - Psyr_0440 0.63 -0.3 477,984 + Psyr_0440 0.83 -0.5 477,984 + Psyr_0440 0.83 +0.8
Or see this region's nucleotide sequence