Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25325 and AO356_25330 are separated by 3 nucleotides AO356_25330 and AO356_25335 overlap by 4 nucleotides AO356_25335 and AO356_25340 are separated by 179 nucleotides
AO356_25325: AO356_25325 - FAD-dependent oxidoreductase, at 5,730,178 to 5,731,425
_25325
AO356_25330: AO356_25330 - short-chain dehydrogenase, at 5,731,429 to 5,732,184
_25330
AO356_25335: AO356_25335 - transcriptional regulator, at 5,732,181 to 5,732,603
_25335
AO356_25340: AO356_25340 - NADH:flavin oxidoreductase, at 5,732,783 to 5,733,868
_25340
Position (kb)
5731
5732
5733 Strain fitness (log2 ratio)
-1
0
1
2 at 5730.452 kb on + strand, within AO356_25325 at 5730.453 kb on - strand, within AO356_25325 at 5730.453 kb on - strand, within AO356_25325 at 5730.494 kb on + strand, within AO356_25325 at 5730.494 kb on + strand, within AO356_25325 at 5730.783 kb on + strand, within AO356_25325 at 5730.783 kb on + strand, within AO356_25325 at 5730.784 kb on - strand, within AO356_25325 at 5730.784 kb on - strand, within AO356_25325 at 5730.784 kb on - strand, within AO356_25325 at 5730.784 kb on - strand, within AO356_25325 at 5730.784 kb on - strand, within AO356_25325 at 5730.785 kb on + strand, within AO356_25325 at 5730.786 kb on - strand, within AO356_25325 at 5730.930 kb on + strand, within AO356_25325 at 5730.930 kb on + strand, within AO356_25325 at 5730.931 kb on - strand, within AO356_25325 at 5730.941 kb on + strand, within AO356_25325 at 5730.941 kb on + strand, within AO356_25325 at 5730.942 kb on - strand, within AO356_25325 at 5730.942 kb on - strand, within AO356_25325 at 5730.983 kb on + strand, within AO356_25325 at 5730.984 kb on - strand, within AO356_25325 at 5731.139 kb on + strand, within AO356_25325 at 5731.140 kb on - strand, within AO356_25325 at 5731.140 kb on - strand, within AO356_25325 at 5731.140 kb on - strand, within AO356_25325 at 5731.185 kb on + strand, within AO356_25325 at 5731.236 kb on - strand, within AO356_25325 at 5731.250 kb on + strand, within AO356_25325 at 5731.250 kb on + strand, within AO356_25325 at 5731.251 kb on - strand, within AO356_25325 at 5731.251 kb on - strand, within AO356_25325 at 5731.376 kb on + strand at 5731.376 kb on + strand at 5731.377 kb on - strand at 5731.507 kb on + strand, within AO356_25330 at 5731.508 kb on - strand, within AO356_25330 at 5731.573 kb on - strand, within AO356_25330 at 5731.823 kb on - strand, within AO356_25330 at 5731.823 kb on - strand, within AO356_25330 at 5731.922 kb on + strand, within AO356_25330 at 5731.922 kb on + strand, within AO356_25330 at 5731.922 kb on + strand, within AO356_25330 at 5731.923 kb on - strand, within AO356_25330 at 5731.923 kb on - strand, within AO356_25330 at 5732.239 kb on + strand, within AO356_25335 at 5732.240 kb on - strand, within AO356_25335 at 5732.278 kb on + strand, within AO356_25335 at 5732.377 kb on + strand, within AO356_25335 at 5732.443 kb on + strand, within AO356_25335 at 5732.444 kb on - strand, within AO356_25335 at 5732.444 kb on - strand, within AO356_25335 at 5732.525 kb on - strand, within AO356_25335 at 5732.606 kb on + strand at 5732.607 kb on - strand at 5732.658 kb on - strand at 5732.658 kb on - strand at 5732.658 kb on - strand at 5732.658 kb on - strand at 5732.670 kb on - strand at 5732.672 kb on - strand at 5732.672 kb on - strand at 5732.823 kb on + strand at 5732.824 kb on - strand at 5732.844 kb on + strand at 5732.844 kb on + strand at 5732.844 kb on + strand at 5732.844 kb on + strand at 5732.845 kb on - strand at 5732.845 kb on - strand at 5733.078 kb on + strand, within AO356_25340
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,730,452 + AO356_25325 0.22 +0.6 5,730,453 - AO356_25325 0.22 -1.4 5,730,453 - AO356_25325 0.22 -1.3 5,730,494 + AO356_25325 0.25 -1.0 5,730,494 + AO356_25325 0.25 +1.0 5,730,783 + AO356_25325 0.48 +0.7 5,730,783 + AO356_25325 0.48 -0.3 5,730,784 - AO356_25325 0.49 +0.2 5,730,784 - AO356_25325 0.49 -0.3 5,730,784 - AO356_25325 0.49 +0.4 5,730,784 - AO356_25325 0.49 -0.1 5,730,784 - AO356_25325 0.49 -0.9 5,730,785 + AO356_25325 0.49 +0.2 5,730,786 - AO356_25325 0.49 +0.0 5,730,930 + AO356_25325 0.60 +1.1 5,730,930 + AO356_25325 0.60 -1.1 5,730,931 - AO356_25325 0.60 +0.7 5,730,941 + AO356_25325 0.61 -0.4 5,730,941 + AO356_25325 0.61 -1.0 5,730,942 - AO356_25325 0.61 +0.4 5,730,942 - AO356_25325 0.61 -0.2 5,730,983 + AO356_25325 0.65 -0.8 5,730,984 - AO356_25325 0.65 +0.6 5,731,139 + AO356_25325 0.77 -0.8 5,731,140 - AO356_25325 0.77 -1.0 5,731,140 - AO356_25325 0.77 +0.5 5,731,140 - AO356_25325 0.77 +0.9 5,731,185 + AO356_25325 0.81 -0.6 5,731,236 - AO356_25325 0.85 +0.2 5,731,250 + AO356_25325 0.86 +0.3 5,731,250 + AO356_25325 0.86 +0.1 5,731,251 - AO356_25325 0.86 +0.5 5,731,251 - AO356_25325 0.86 -0.6 5,731,376 + -0.8 5,731,376 + +1.4 5,731,377 - +0.3 5,731,507 + AO356_25330 0.10 -1.0 5,731,508 - AO356_25330 0.10 -0.7 5,731,573 - AO356_25330 0.19 -0.1 5,731,823 - AO356_25330 0.52 +0.3 5,731,823 - AO356_25330 0.52 +0.2 5,731,922 + AO356_25330 0.65 +1.4 5,731,922 + AO356_25330 0.65 +1.8 5,731,922 + AO356_25330 0.65 -0.1 5,731,923 - AO356_25330 0.65 -0.0 5,731,923 - AO356_25330 0.65 +0.5 5,732,239 + AO356_25335 0.14 -0.5 5,732,240 - AO356_25335 0.14 +0.4 5,732,278 + AO356_25335 0.23 -0.0 5,732,377 + AO356_25335 0.46 -1.5 5,732,443 + AO356_25335 0.62 +0.5 5,732,444 - AO356_25335 0.62 +0.2 5,732,444 - AO356_25335 0.62 -0.8 5,732,525 - AO356_25335 0.81 -0.2 5,732,606 + +0.7 5,732,607 - -0.1 5,732,658 - +0.4 5,732,658 - +0.2 5,732,658 - -0.3 5,732,658 - -0.8 5,732,670 - +1.4 5,732,672 - +0.8 5,732,672 - -0.8 5,732,823 + +0.9 5,732,824 - -0.1 5,732,844 + +0.6 5,732,844 + -0.2 5,732,844 + -0.1 5,732,844 + +1.4 5,732,845 - +2.4 5,732,845 - -0.1 5,733,078 + AO356_25340 0.27 +1.0
Or see this region's nucleotide sequence