Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25300 and AO356_25305 overlap by 4 nucleotides AO356_25305 and AO356_25310 are separated by 21 nucleotides AO356_25310 and AO356_25315 are separated by 5 nucleotides AO356_25315 and AO356_25320 overlap by 29 nucleotides AO356_25320 and AO356_25325 overlap by 4 nucleotides
AO356_25300: AO356_25300 - hypothetical protein, at 5,726,775 to 5,727,314
_25300
AO356_25305: AO356_25305 - metal ABC transporter ATPase, at 5,727,311 to 5,727,616
_25305
AO356_25310: AO356_25310 - hypothetical protein, at 5,727,638 to 5,728,126
_25310
AO356_25315: AO356_25315 - cyclopropane-fatty-acyl-phospholipid synthase, at 5,728,132 to 5,729,394
_25315
AO356_25320: AO356_25320 - hypothetical protein, at 5,729,366 to 5,730,181
_25320
AO356_25325: AO356_25325 - FAD-dependent oxidoreductase, at 5,730,178 to 5,731,425
_25325
Position (kb) 5728
5729
5730 Strain fitness (log2 ratio) -1
0
1 at 5727.177 kb on + strand, within AO356_25300 at 5727.177 kb on + strand, within AO356_25300 at 5727.177 kb on + strand, within AO356_25300 at 5727.178 kb on - strand, within AO356_25300 at 5727.205 kb on - strand, within AO356_25300 at 5727.205 kb on - strand, within AO356_25300 at 5727.393 kb on - strand, within AO356_25305 at 5727.393 kb on - strand, within AO356_25305 at 5727.396 kb on + strand, within AO356_25305 at 5727.396 kb on + strand, within AO356_25305 at 5727.396 kb on + strand, within AO356_25305 at 5727.397 kb on - strand, within AO356_25305 at 5727.397 kb on - strand, within AO356_25305 at 5727.397 kb on - strand, within AO356_25305 at 5727.427 kb on + strand, within AO356_25305 at 5727.427 kb on + strand, within AO356_25305 at 5727.428 kb on - strand, within AO356_25305 at 5727.428 kb on - strand, within AO356_25305 at 5727.615 kb on - strand at 5727.677 kb on + strand at 5727.677 kb on + strand at 5727.678 kb on - strand at 5727.762 kb on + strand, within AO356_25310 at 5727.763 kb on - strand, within AO356_25310 at 5727.833 kb on - strand, within AO356_25310 at 5728.133 kb on + strand at 5728.230 kb on - strand at 5728.230 kb on - strand at 5728.238 kb on + strand at 5728.239 kb on - strand at 5728.271 kb on + strand, within AO356_25315 at 5728.340 kb on + strand, within AO356_25315 at 5728.340 kb on + strand, within AO356_25315 at 5728.341 kb on - strand, within AO356_25315 at 5728.563 kb on - strand, within AO356_25315 at 5728.622 kb on + strand, within AO356_25315 at 5728.754 kb on + strand, within AO356_25315 at 5728.754 kb on + strand, within AO356_25315 at 5728.754 kb on + strand, within AO356_25315 at 5728.754 kb on + strand, within AO356_25315 at 5728.754 kb on + strand, within AO356_25315 at 5728.754 kb on + strand, within AO356_25315 at 5728.754 kb on + strand, within AO356_25315 at 5728.755 kb on - strand, within AO356_25315 at 5728.913 kb on + strand, within AO356_25315 at 5728.965 kb on - strand, within AO356_25315 at 5728.965 kb on - strand, within AO356_25315 at 5728.977 kb on + strand, within AO356_25315 at 5728.977 kb on + strand, within AO356_25315 at 5729.147 kb on + strand, within AO356_25315 at 5729.147 kb on + strand, within AO356_25315 at 5729.147 kb on + strand, within AO356_25315 at 5729.147 kb on + strand, within AO356_25315 at 5729.147 kb on + strand, within AO356_25315 at 5729.148 kb on - strand, within AO356_25315 at 5729.148 kb on - strand, within AO356_25315 at 5729.148 kb on - strand, within AO356_25315 at 5729.148 kb on - strand, within AO356_25315 at 5729.148 kb on - strand, within AO356_25315 at 5729.425 kb on + strand at 5729.499 kb on + strand, within AO356_25320 at 5729.499 kb on + strand, within AO356_25320 at 5729.679 kb on + strand, within AO356_25320 at 5729.769 kb on + strand, within AO356_25320 at 5729.769 kb on + strand, within AO356_25320 at 5729.769 kb on + strand, within AO356_25320 at 5729.770 kb on - strand, within AO356_25320 at 5729.770 kb on - strand, within AO356_25320 at 5729.770 kb on - strand, within AO356_25320 at 5729.794 kb on + strand, within AO356_25320 at 5729.794 kb on + strand, within AO356_25320 at 5729.794 kb on + strand, within AO356_25320 at 5729.795 kb on - strand, within AO356_25320 at 5729.797 kb on + strand, within AO356_25320 at 5729.797 kb on + strand, within AO356_25320 at 5730.093 kb on + strand, within AO356_25320 at 5730.269 kb on + strand at 5730.269 kb on + strand at 5730.270 kb on - strand at 5730.270 kb on - strand at 5730.333 kb on - strand, within AO356_25325 at 5730.333 kb on - strand, within AO356_25325
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,727,177 + AO356_25300 0.74 +0.2 5,727,177 + AO356_25300 0.74 +0.6 5,727,177 + AO356_25300 0.74 -0.8 5,727,178 - AO356_25300 0.75 +0.4 5,727,205 - AO356_25300 0.80 +0.1 5,727,205 - AO356_25300 0.80 -0.5 5,727,393 - AO356_25305 0.27 +0.4 5,727,393 - AO356_25305 0.27 -0.1 5,727,396 + AO356_25305 0.28 +0.0 5,727,396 + AO356_25305 0.28 -0.1 5,727,396 + AO356_25305 0.28 -0.7 5,727,397 - AO356_25305 0.28 +1.0 5,727,397 - AO356_25305 0.28 -0.5 5,727,397 - AO356_25305 0.28 +0.1 5,727,427 + AO356_25305 0.38 +0.5 5,727,427 + AO356_25305 0.38 +0.5 5,727,428 - AO356_25305 0.38 +0.8 5,727,428 - AO356_25305 0.38 -0.2 5,727,615 - +1.0 5,727,677 + +0.0 5,727,677 + -0.6 5,727,678 - -0.2 5,727,762 + AO356_25310 0.25 -1.2 5,727,763 - AO356_25310 0.26 -0.9 5,727,833 - AO356_25310 0.40 -0.4 5,728,133 + -0.4 5,728,230 - -0.4 5,728,230 - +0.3 5,728,238 + -0.2 5,728,239 - +0.2 5,728,271 + AO356_25315 0.11 -0.3 5,728,340 + AO356_25315 0.16 +0.4 5,728,340 + AO356_25315 0.16 -0.9 5,728,341 - AO356_25315 0.17 -0.6 5,728,563 - AO356_25315 0.34 -0.5 5,728,622 + AO356_25315 0.39 -0.4 5,728,754 + AO356_25315 0.49 +0.1 5,728,754 + AO356_25315 0.49 -0.3 5,728,754 + AO356_25315 0.49 +0.8 5,728,754 + AO356_25315 0.49 +0.3 5,728,754 + AO356_25315 0.49 +0.9 5,728,754 + AO356_25315 0.49 +0.3 5,728,754 + AO356_25315 0.49 +1.3 5,728,755 - AO356_25315 0.49 +1.2 5,728,913 + AO356_25315 0.62 -1.1 5,728,965 - AO356_25315 0.66 +0.3 5,728,965 - AO356_25315 0.66 -0.1 5,728,977 + AO356_25315 0.67 -0.1 5,728,977 + AO356_25315 0.67 +0.0 5,729,147 + AO356_25315 0.80 -0.7 5,729,147 + AO356_25315 0.80 +0.1 5,729,147 + AO356_25315 0.80 +1.5 5,729,147 + AO356_25315 0.80 -0.1 5,729,147 + AO356_25315 0.80 +0.4 5,729,148 - AO356_25315 0.80 +0.4 5,729,148 - AO356_25315 0.80 -0.1 5,729,148 - AO356_25315 0.80 -0.3 5,729,148 - AO356_25315 0.80 +0.4 5,729,148 - AO356_25315 0.80 -0.7 5,729,425 + +0.5 5,729,499 + AO356_25320 0.16 -0.6 5,729,499 + AO356_25320 0.16 +0.3 5,729,679 + AO356_25320 0.38 -0.2 5,729,769 + AO356_25320 0.49 +0.2 5,729,769 + AO356_25320 0.49 +0.3 5,729,769 + AO356_25320 0.49 -0.3 5,729,770 - AO356_25320 0.50 -0.4 5,729,770 - AO356_25320 0.50 -1.0 5,729,770 - AO356_25320 0.50 +0.4 5,729,794 + AO356_25320 0.52 +0.2 5,729,794 + AO356_25320 0.52 -0.9 5,729,794 + AO356_25320 0.52 +0.0 5,729,795 - AO356_25320 0.53 +0.9 5,729,797 + AO356_25320 0.53 +0.2 5,729,797 + AO356_25320 0.53 +0.7 5,730,093 + AO356_25320 0.89 -0.8 5,730,269 + -0.9 5,730,269 + -0.6 5,730,270 - +0.3 5,730,270 - -1.2 5,730,333 - AO356_25325 0.12 -1.0 5,730,333 - AO356_25325 0.12 -0.6
Or see this region's nucleotide sequence