Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_24645 and AO356_24650 overlap by 4 nucleotides AO356_24650 and AO356_24655 overlap by 8 nucleotides AO356_24655 and AO356_24660 overlap by 4 nucleotides
AO356_24645: AO356_24645 - hypothetical protein, at 5,573,955 to 5,575,691
_24645
AO356_24650: AO356_24650 - BatB protein, at 5,575,688 to 5,576,767
_24650
AO356_24655: AO356_24655 - hypothetical protein, at 5,576,760 to 5,577,254
_24655
AO356_24660: AO356_24660 - hypothetical protein, at 5,577,251 to 5,578,195
_24660
Position (kb)
5575
5576
5577 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5574.967 kb on + strand, within AO356_24645 at 5574.967 kb on + strand, within AO356_24645 at 5574.967 kb on + strand, within AO356_24645 at 5574.968 kb on - strand, within AO356_24645 at 5574.968 kb on - strand, within AO356_24645 at 5574.968 kb on - strand, within AO356_24645 at 5575.276 kb on + strand, within AO356_24645 at 5575.277 kb on - strand, within AO356_24645 at 5575.326 kb on + strand, within AO356_24645 at 5575.399 kb on - strand, within AO356_24645 at 5575.820 kb on + strand, within AO356_24650 at 5575.820 kb on + strand, within AO356_24650 at 5575.820 kb on + strand, within AO356_24650 at 5575.821 kb on - strand, within AO356_24650 at 5575.821 kb on - strand, within AO356_24650 at 5575.858 kb on - strand, within AO356_24650 at 5575.858 kb on - strand, within AO356_24650 at 5575.966 kb on - strand, within AO356_24650 at 5576.138 kb on + strand, within AO356_24650 at 5576.139 kb on - strand, within AO356_24650 at 5576.331 kb on + strand, within AO356_24650 at 5576.582 kb on - strand, within AO356_24650 at 5576.760 kb on + strand at 5576.843 kb on - strand, within AO356_24655 at 5576.843 kb on - strand, within AO356_24655 at 5576.843 kb on - strand, within AO356_24655 at 5576.859 kb on + strand, within AO356_24655 at 5576.860 kb on - strand, within AO356_24655 at 5577.172 kb on - strand, within AO356_24655 at 5577.286 kb on - strand at 5577.294 kb on - strand at 5577.320 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,574,967 + AO356_24645 0.58 -2.0 5,574,967 + AO356_24645 0.58 -1.5 5,574,967 + AO356_24645 0.58 -4.0 5,574,968 - AO356_24645 0.58 -2.5 5,574,968 - AO356_24645 0.58 -3.4 5,574,968 - AO356_24645 0.58 -1.8 5,575,276 + AO356_24645 0.76 -2.3 5,575,277 - AO356_24645 0.76 -2.9 5,575,326 + AO356_24645 0.79 -2.7 5,575,399 - AO356_24645 0.83 -0.1 5,575,820 + AO356_24650 0.12 -1.1 5,575,820 + AO356_24650 0.12 -2.0 5,575,820 + AO356_24650 0.12 -2.1 5,575,821 - AO356_24650 0.12 -0.2 5,575,821 - AO356_24650 0.12 +1.2 5,575,858 - AO356_24650 0.16 -1.1 5,575,858 - AO356_24650 0.16 -1.8 5,575,966 - AO356_24650 0.26 -3.0 5,576,138 + AO356_24650 0.42 -1.7 5,576,139 - AO356_24650 0.42 -1.4 5,576,331 + AO356_24650 0.60 -1.5 5,576,582 - AO356_24650 0.83 -2.2 5,576,760 + -2.0 5,576,843 - AO356_24655 0.17 -1.7 5,576,843 - AO356_24655 0.17 -1.5 5,576,843 - AO356_24655 0.17 -1.0 5,576,859 + AO356_24655 0.20 -2.0 5,576,860 - AO356_24655 0.20 -3.5 5,577,172 - AO356_24655 0.83 -1.0 5,577,286 - +0.7 5,577,294 - +1.0 5,577,320 - -3.3
Or see this region's nucleotide sequence