Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_24420 and AO356_24425 are separated by 146 nucleotides AO356_24425 and AO356_24430 are separated by 175 nucleotides AO356_24430 and AO356_24435 are separated by 55 nucleotides
AO356_24420: AO356_24420 - XRE family transcriptional regulator, at 5,514,206 to 5,515,288
_24420
AO356_24425: AO356_24425 - hypothetical protein, at 5,515,435 to 5,515,923
_24425
AO356_24430: AO356_24430 - lysine transporter LysE, at 5,516,099 to 5,516,686
_24430
AO356_24435: AO356_24435 - AraC family transcriptional regulator, at 5,516,742 to 5,517,566
_24435
Position (kb)
5516
5517 Strain fitness (log2 ratio)
-1
0
1
2 at 5515.215 kb on + strand at 5515.216 kb on - strand at 5515.300 kb on + strand at 5515.300 kb on + strand at 5515.300 kb on + strand at 5515.300 kb on + strand at 5515.301 kb on - strand at 5515.301 kb on - strand at 5515.301 kb on - strand at 5515.447 kb on - strand at 5515.447 kb on - strand at 5515.447 kb on - strand at 5515.656 kb on + strand, within AO356_24425 at 5515.657 kb on - strand, within AO356_24425 at 5516.059 kb on + strand at 5516.059 kb on + strand at 5516.059 kb on + strand at 5516.060 kb on - strand at 5516.060 kb on - strand at 5516.060 kb on - strand at 5516.082 kb on + strand at 5516.082 kb on + strand at 5516.082 kb on + strand at 5516.082 kb on + strand at 5516.082 kb on + strand at 5516.083 kb on - strand at 5516.147 kb on - strand at 5516.147 kb on - strand at 5516.286 kb on + strand, within AO356_24430 at 5516.286 kb on + strand, within AO356_24430 at 5516.286 kb on + strand, within AO356_24430 at 5516.286 kb on + strand, within AO356_24430 at 5516.421 kb on - strand, within AO356_24430 at 5516.535 kb on - strand, within AO356_24430 at 5516.550 kb on - strand, within AO356_24430 at 5516.593 kb on + strand, within AO356_24430 at 5516.594 kb on - strand, within AO356_24430 at 5516.669 kb on + strand at 5516.670 kb on - strand at 5516.670 kb on - strand at 5516.670 kb on - strand at 5516.721 kb on + strand at 5516.721 kb on + strand at 5516.721 kb on + strand at 5516.722 kb on - strand at 5516.722 kb on - strand at 5516.725 kb on + strand at 5516.725 kb on + strand at 5516.726 kb on - strand at 5516.726 kb on - strand at 5516.765 kb on + strand at 5517.017 kb on + strand, within AO356_24435 at 5517.018 kb on - strand, within AO356_24435 at 5517.018 kb on - strand, within AO356_24435 at 5517.163 kb on + strand, within AO356_24435 at 5517.163 kb on + strand, within AO356_24435 at 5517.163 kb on + strand, within AO356_24435 at 5517.163 kb on + strand, within AO356_24435 at 5517.163 kb on + strand, within AO356_24435 at 5517.163 kb on + strand, within AO356_24435 at 5517.164 kb on - strand, within AO356_24435 at 5517.164 kb on - strand, within AO356_24435 at 5517.164 kb on - strand, within AO356_24435 at 5517.164 kb on - strand, within AO356_24435 at 5517.164 kb on - strand, within AO356_24435 at 5517.164 kb on - strand, within AO356_24435 at 5517.164 kb on - strand, within AO356_24435 at 5517.165 kb on + strand, within AO356_24435 at 5517.166 kb on - strand, within AO356_24435 at 5517.289 kb on + strand, within AO356_24435 at 5517.289 kb on + strand, within AO356_24435 at 5517.289 kb on + strand, within AO356_24435 at 5517.301 kb on + strand, within AO356_24435 at 5517.302 kb on - strand, within AO356_24435 at 5517.302 kb on - strand, within AO356_24435 at 5517.302 kb on - strand, within AO356_24435 at 5517.302 kb on - strand, within AO356_24435 at 5517.406 kb on + strand, within AO356_24435 at 5517.406 kb on + strand, within AO356_24435 at 5517.406 kb on + strand, within AO356_24435 at 5517.406 kb on + strand, within AO356_24435 at 5517.407 kb on - strand, within AO356_24435 at 5517.407 kb on - strand, within AO356_24435 at 5517.466 kb on + strand, within AO356_24435 at 5517.603 kb on + strand at 5517.603 kb on + strand at 5517.604 kb on - strand at 5517.606 kb on + strand at 5517.656 kb on + strand at 5517.656 kb on + strand at 5517.670 kb on + strand at 5517.671 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,515,215 + -0.1 5,515,216 - -0.8 5,515,300 + -0.1 5,515,300 + +1.0 5,515,300 + +0.8 5,515,300 + -0.1 5,515,301 - +0.2 5,515,301 - -0.4 5,515,301 - -0.4 5,515,447 - +1.1 5,515,447 - -0.7 5,515,447 - -0.9 5,515,656 + AO356_24425 0.45 +0.3 5,515,657 - AO356_24425 0.45 +1.0 5,516,059 + +0.3 5,516,059 + -1.3 5,516,059 + -0.1 5,516,060 - -0.4 5,516,060 - +0.2 5,516,060 - -0.8 5,516,082 + +0.5 5,516,082 + -0.1 5,516,082 + +0.2 5,516,082 + -0.6 5,516,082 + +0.1 5,516,083 - -0.2 5,516,147 - +0.2 5,516,147 - +0.1 5,516,286 + AO356_24430 0.32 +0.5 5,516,286 + AO356_24430 0.32 -0.1 5,516,286 + AO356_24430 0.32 +0.1 5,516,286 + AO356_24430 0.32 +1.1 5,516,421 - AO356_24430 0.55 +0.2 5,516,535 - AO356_24430 0.74 +0.8 5,516,550 - AO356_24430 0.77 +0.6 5,516,593 + AO356_24430 0.84 -0.5 5,516,594 - AO356_24430 0.84 +0.4 5,516,669 + +0.6 5,516,670 - -0.7 5,516,670 - -0.3 5,516,670 - +0.7 5,516,721 + -1.4 5,516,721 + +0.2 5,516,721 + +0.4 5,516,722 - -0.8 5,516,722 - +0.6 5,516,725 + -0.1 5,516,725 + +0.2 5,516,726 - +0.7 5,516,726 - +0.8 5,516,765 + +0.7 5,517,017 + AO356_24435 0.33 -0.9 5,517,018 - AO356_24435 0.33 +0.9 5,517,018 - AO356_24435 0.33 +0.5 5,517,163 + AO356_24435 0.51 +0.4 5,517,163 + AO356_24435 0.51 +0.1 5,517,163 + AO356_24435 0.51 +0.3 5,517,163 + AO356_24435 0.51 +0.5 5,517,163 + AO356_24435 0.51 +0.1 5,517,163 + AO356_24435 0.51 -0.0 5,517,164 - AO356_24435 0.51 +0.1 5,517,164 - AO356_24435 0.51 +0.1 5,517,164 - AO356_24435 0.51 -0.6 5,517,164 - AO356_24435 0.51 -1.2 5,517,164 - AO356_24435 0.51 -0.5 5,517,164 - AO356_24435 0.51 +1.6 5,517,164 - AO356_24435 0.51 +0.2 5,517,165 + AO356_24435 0.51 -1.6 5,517,166 - AO356_24435 0.51 +0.4 5,517,289 + AO356_24435 0.66 +1.0 5,517,289 + AO356_24435 0.66 +0.3 5,517,289 + AO356_24435 0.66 -0.2 5,517,301 + AO356_24435 0.68 -0.3 5,517,302 - AO356_24435 0.68 -0.4 5,517,302 - AO356_24435 0.68 +1.1 5,517,302 - AO356_24435 0.68 -0.1 5,517,302 - AO356_24435 0.68 -0.0 5,517,406 + AO356_24435 0.80 -0.8 5,517,406 + AO356_24435 0.80 +1.9 5,517,406 + AO356_24435 0.80 +0.0 5,517,406 + AO356_24435 0.80 -0.4 5,517,407 - AO356_24435 0.81 -0.3 5,517,407 - AO356_24435 0.81 +0.0 5,517,466 + AO356_24435 0.88 +0.4 5,517,603 + +1.3 5,517,603 + +0.6 5,517,604 - -0.4 5,517,606 + +0.1 5,517,656 + +0.8 5,517,656 + -0.5 5,517,670 + +1.1 5,517,671 - +1.0
Or see this region's nucleotide sequence