Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22655 and AO356_22660 overlap by 4 nucleotides AO356_22660 and AO356_22665 are separated by 39 nucleotides AO356_22665 and AO356_22670 are separated by 33 nucleotides
AO356_22655: AO356_22655 - SAM-dependent methyltransferase, at 5,062,288 to 5,063,124
_22655
AO356_22660: AO356_22660 - chemotaxis protein CheW, at 5,063,121 to 5,063,657
_22660
AO356_22665: AO356_22665 - chemotaxis protein, at 5,063,697 to 5,065,355
_22665
AO356_22670: AO356_22670 - chemotaxis protein CheA, at 5,065,389 to 5,067,491
_22670
Position (kb)
5063
5064
5065
5066 Strain fitness (log2 ratio)
-1
0
1
2 at 5062.741 kb on - strand, within AO356_22655 at 5062.741 kb on - strand, within AO356_22655 at 5062.742 kb on + strand, within AO356_22655 at 5062.742 kb on + strand, within AO356_22655 at 5062.742 kb on + strand, within AO356_22655 at 5062.743 kb on - strand, within AO356_22655 at 5062.743 kb on - strand, within AO356_22655 at 5062.743 kb on - strand, within AO356_22655 at 5062.813 kb on + strand, within AO356_22655 at 5062.813 kb on + strand, within AO356_22655 at 5062.814 kb on - strand, within AO356_22655 at 5062.814 kb on - strand, within AO356_22655 at 5062.814 kb on - strand, within AO356_22655 at 5062.814 kb on - strand, within AO356_22655 at 5062.897 kb on + strand, within AO356_22655 at 5062.897 kb on + strand, within AO356_22655 at 5062.898 kb on - strand, within AO356_22655 at 5062.898 kb on - strand, within AO356_22655 at 5062.937 kb on + strand, within AO356_22655 at 5062.938 kb on - strand, within AO356_22655 at 5063.113 kb on - strand at 5063.113 kb on - strand at 5063.588 kb on + strand, within AO356_22660 at 5063.589 kb on - strand, within AO356_22660 at 5063.599 kb on + strand, within AO356_22660 at 5063.599 kb on + strand, within AO356_22660 at 5063.599 kb on + strand, within AO356_22660 at 5063.600 kb on - strand, within AO356_22660 at 5063.600 kb on - strand, within AO356_22660 at 5063.600 kb on - strand, within AO356_22660 at 5063.672 kb on + strand at 5063.673 kb on - strand at 5063.673 kb on - strand at 5063.744 kb on - strand at 5063.866 kb on + strand, within AO356_22665 at 5064.552 kb on - strand, within AO356_22665 at 5064.979 kb on + strand, within AO356_22665 at 5064.980 kb on - strand, within AO356_22665 at 5065.049 kb on - strand, within AO356_22665 at 5065.049 kb on - strand, within AO356_22665 at 5065.334 kb on + strand at 5065.334 kb on + strand at 5065.335 kb on - strand at 5065.335 kb on - strand at 5065.335 kb on - strand at 5065.335 kb on - strand at 5065.342 kb on + strand at 5065.343 kb on - strand at 5065.343 kb on - strand at 5065.343 kb on - strand at 5065.343 kb on - strand at 5065.343 kb on - strand at 5065.363 kb on + strand at 5065.363 kb on + strand at 5065.363 kb on + strand at 5065.363 kb on + strand at 5065.364 kb on - strand at 5065.364 kb on - strand at 5065.364 kb on - strand at 5065.382 kb on + strand at 5065.382 kb on + strand at 5065.382 kb on + strand at 5065.382 kb on + strand at 5065.382 kb on + strand at 5065.410 kb on + strand at 5065.410 kb on + strand at 5065.410 kb on + strand at 5065.411 kb on - strand at 5065.411 kb on - strand at 5065.411 kb on - strand at 5065.411 kb on - strand at 5065.468 kb on - strand at 5065.766 kb on + strand, within AO356_22670 at 5065.767 kb on - strand, within AO356_22670 at 5065.798 kb on + strand, within AO356_22670 at 5065.798 kb on + strand, within AO356_22670 at 5065.798 kb on + strand, within AO356_22670 at 5065.798 kb on + strand, within AO356_22670 at 5065.798 kb on + strand, within AO356_22670 at 5065.798 kb on + strand, within AO356_22670 at 5065.799 kb on - strand, within AO356_22670 at 5066.017 kb on + strand, within AO356_22670 at 5066.018 kb on - strand, within AO356_22670 at 5066.018 kb on - strand, within AO356_22670 at 5066.150 kb on - strand, within AO356_22670 at 5066.150 kb on - strand, within AO356_22670 at 5066.214 kb on + strand, within AO356_22670 at 5066.215 kb on - strand, within AO356_22670 at 5066.215 kb on - strand, within AO356_22670
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,062,741 - AO356_22655 0.54 +0.5 5,062,741 - AO356_22655 0.54 +0.2 5,062,742 + AO356_22655 0.54 +0.2 5,062,742 + AO356_22655 0.54 -0.7 5,062,742 + AO356_22655 0.54 -0.3 5,062,743 - AO356_22655 0.54 +0.2 5,062,743 - AO356_22655 0.54 +0.0 5,062,743 - AO356_22655 0.54 -0.2 5,062,813 + AO356_22655 0.63 +0.9 5,062,813 + AO356_22655 0.63 -1.3 5,062,814 - AO356_22655 0.63 -0.1 5,062,814 - AO356_22655 0.63 +0.5 5,062,814 - AO356_22655 0.63 -1.0 5,062,814 - AO356_22655 0.63 +0.5 5,062,897 + AO356_22655 0.73 +0.1 5,062,897 + AO356_22655 0.73 -0.7 5,062,898 - AO356_22655 0.73 -0.3 5,062,898 - AO356_22655 0.73 +2.0 5,062,937 + AO356_22655 0.78 +0.3 5,062,938 - AO356_22655 0.78 +0.4 5,063,113 - -0.2 5,063,113 - -0.2 5,063,588 + AO356_22660 0.87 +0.2 5,063,589 - AO356_22660 0.87 +0.4 5,063,599 + AO356_22660 0.89 +0.5 5,063,599 + AO356_22660 0.89 +0.8 5,063,599 + AO356_22660 0.89 +0.4 5,063,600 - AO356_22660 0.89 +0.8 5,063,600 - AO356_22660 0.89 +1.4 5,063,600 - AO356_22660 0.89 +0.9 5,063,672 + +0.4 5,063,673 - +1.2 5,063,673 - +0.3 5,063,744 - -0.5 5,063,866 + AO356_22665 0.10 -1.2 5,064,552 - AO356_22665 0.52 -0.3 5,064,979 + AO356_22665 0.77 -0.1 5,064,980 - AO356_22665 0.77 +0.6 5,065,049 - AO356_22665 0.81 +0.2 5,065,049 - AO356_22665 0.81 +0.0 5,065,334 + -0.1 5,065,334 + +0.3 5,065,335 - -0.2 5,065,335 - +0.4 5,065,335 - -0.8 5,065,335 - -0.7 5,065,342 + +0.7 5,065,343 - -0.1 5,065,343 - -0.1 5,065,343 - +0.1 5,065,343 - -0.1 5,065,343 - +0.4 5,065,363 + -0.1 5,065,363 + +0.6 5,065,363 + -0.5 5,065,363 + +0.0 5,065,364 - -0.1 5,065,364 - -0.0 5,065,364 - +0.7 5,065,382 + +0.0 5,065,382 + +0.2 5,065,382 + +0.0 5,065,382 + +0.7 5,065,382 + +0.7 5,065,410 + -1.6 5,065,410 + +1.7 5,065,410 + -0.2 5,065,411 - +0.1 5,065,411 - -1.0 5,065,411 - -1.6 5,065,411 - +0.0 5,065,468 - -1.4 5,065,766 + AO356_22670 0.18 -0.5 5,065,767 - AO356_22670 0.18 +0.5 5,065,798 + AO356_22670 0.19 -0.3 5,065,798 + AO356_22670 0.19 +0.0 5,065,798 + AO356_22670 0.19 +0.2 5,065,798 + AO356_22670 0.19 -0.6 5,065,798 + AO356_22670 0.19 -0.2 5,065,798 + AO356_22670 0.19 -0.4 5,065,799 - AO356_22670 0.19 +0.0 5,066,017 + AO356_22670 0.30 -1.7 5,066,018 - AO356_22670 0.30 +1.9 5,066,018 - AO356_22670 0.30 +1.1 5,066,150 - AO356_22670 0.36 -0.7 5,066,150 - AO356_22670 0.36 -0.6 5,066,214 + AO356_22670 0.39 -0.5 5,066,215 - AO356_22670 0.39 +0.2 5,066,215 - AO356_22670 0.39 +0.4
Or see this region's nucleotide sequence