Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22175 and AO356_22180 are separated by 58 nucleotides AO356_22180 and AO356_22185 are separated by 214 nucleotides AO356_22185 and AO356_22190 overlap by 4 nucleotides AO356_22190 and AO356_22195 are separated by 15 nucleotides
AO356_22175: AO356_22175 - cell division protein FtsK, at 4,963,107 to 4,965,512
_22175
AO356_22180: AO356_22180 - outer membrane lipoprotein carrier protein LolA, at 4,965,571 to 4,966,194
_22180
AO356_22185: AO356_22185 - recombination factor protein RarA, at 4,966,409 to 4,967,734
_22185
AO356_22190: AO356_22190 - camphor resistance protein CrcB, at 4,967,731 to 4,968,105
_22190
AO356_22195: AO356_22195 - serine--tRNA ligase, at 4,968,121 to 4,969,401
_22195
Position (kb)
4966
4967
4968 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4965.484 kb on + strand at 4965.484 kb on + strand at 4966.192 kb on + strand at 4966.192 kb on + strand at 4966.192 kb on + strand at 4966.193 kb on - strand at 4966.193 kb on - strand at 4966.193 kb on - strand at 4966.193 kb on - strand at 4966.193 kb on - strand at 4966.402 kb on - strand at 4966.405 kb on - strand at 4966.405 kb on - strand at 4966.405 kb on - strand at 4966.405 kb on - strand at 4966.482 kb on - strand at 4966.482 kb on - strand at 4966.712 kb on + strand, within AO356_22185 at 4966.787 kb on + strand, within AO356_22185 at 4966.788 kb on - strand, within AO356_22185 at 4966.880 kb on + strand, within AO356_22185 at 4966.880 kb on + strand, within AO356_22185 at 4966.881 kb on - strand, within AO356_22185 at 4966.881 kb on - strand, within AO356_22185 at 4966.881 kb on - strand, within AO356_22185 at 4966.881 kb on - strand, within AO356_22185 at 4967.154 kb on - strand, within AO356_22185 at 4967.213 kb on + strand, within AO356_22185 at 4967.214 kb on - strand, within AO356_22185 at 4967.255 kb on + strand, within AO356_22185 at 4967.255 kb on + strand, within AO356_22185 at 4967.256 kb on - strand, within AO356_22185 at 4967.256 kb on - strand, within AO356_22185 at 4967.256 kb on - strand, within AO356_22185 at 4967.256 kb on - strand, within AO356_22185 at 4967.402 kb on + strand, within AO356_22185 at 4967.435 kb on + strand, within AO356_22185 at 4967.435 kb on + strand, within AO356_22185 at 4967.435 kb on + strand, within AO356_22185 at 4967.435 kb on + strand, within AO356_22185 at 4967.436 kb on - strand, within AO356_22185 at 4967.436 kb on - strand, within AO356_22185 at 4967.436 kb on - strand, within AO356_22185 at 4967.552 kb on + strand, within AO356_22185 at 4967.553 kb on - strand, within AO356_22185 at 4967.600 kb on + strand, within AO356_22185 at 4967.600 kb on + strand, within AO356_22185 at 4967.600 kb on + strand, within AO356_22185 at 4967.633 kb on + strand at 4967.633 kb on + strand at 4967.744 kb on + strand at 4967.744 kb on + strand at 4967.744 kb on + strand at 4967.833 kb on + strand, within AO356_22190 at 4967.833 kb on + strand, within AO356_22190 at 4967.833 kb on + strand, within AO356_22190 at 4967.833 kb on + strand, within AO356_22190 at 4967.885 kb on + strand, within AO356_22190 at 4967.885 kb on + strand, within AO356_22190 at 4967.885 kb on + strand, within AO356_22190 at 4967.885 kb on + strand, within AO356_22190 at 4967.887 kb on + strand, within AO356_22190 at 4968.037 kb on + strand, within AO356_22190
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,965,484 + +2.4 4,965,484 + -0.2 4,966,192 + -0.1 4,966,192 + +0.5 4,966,192 + -0.0 4,966,193 - +0.8 4,966,193 - -0.3 4,966,193 - -0.1 4,966,193 - +1.5 4,966,193 - +0.4 4,966,402 - -0.3 4,966,405 - +1.3 4,966,405 - -0.4 4,966,405 - -0.6 4,966,405 - +0.4 4,966,482 - +0.4 4,966,482 - -1.8 4,966,712 + AO356_22185 0.23 +0.3 4,966,787 + AO356_22185 0.29 -0.4 4,966,788 - AO356_22185 0.29 +0.0 4,966,880 + AO356_22185 0.36 +0.5 4,966,880 + AO356_22185 0.36 +0.8 4,966,881 - AO356_22185 0.36 +1.6 4,966,881 - AO356_22185 0.36 +0.1 4,966,881 - AO356_22185 0.36 +0.0 4,966,881 - AO356_22185 0.36 -0.1 4,967,154 - AO356_22185 0.56 +0.4 4,967,213 + AO356_22185 0.61 -1.2 4,967,214 - AO356_22185 0.61 +1.0 4,967,255 + AO356_22185 0.64 +1.0 4,967,255 + AO356_22185 0.64 +0.4 4,967,256 - AO356_22185 0.64 +0.4 4,967,256 - AO356_22185 0.64 +2.2 4,967,256 - AO356_22185 0.64 +0.7 4,967,256 - AO356_22185 0.64 -0.7 4,967,402 + AO356_22185 0.75 +1.1 4,967,435 + AO356_22185 0.77 +0.7 4,967,435 + AO356_22185 0.77 -0.5 4,967,435 + AO356_22185 0.77 -1.0 4,967,435 + AO356_22185 0.77 -0.3 4,967,436 - AO356_22185 0.77 +0.9 4,967,436 - AO356_22185 0.77 +0.6 4,967,436 - AO356_22185 0.77 +0.9 4,967,552 + AO356_22185 0.86 -0.3 4,967,553 - AO356_22185 0.86 +0.4 4,967,600 + AO356_22185 0.90 -0.4 4,967,600 + AO356_22185 0.90 +0.5 4,967,600 + AO356_22185 0.90 +1.2 4,967,633 + +1.1 4,967,633 + +0.3 4,967,744 + -0.5 4,967,744 + -0.9 4,967,744 + -0.2 4,967,833 + AO356_22190 0.27 +0.6 4,967,833 + AO356_22190 0.27 -1.9 4,967,833 + AO356_22190 0.27 +0.5 4,967,833 + AO356_22190 0.27 +1.5 4,967,885 + AO356_22190 0.41 -0.4 4,967,885 + AO356_22190 0.41 +0.3 4,967,885 + AO356_22190 0.41 +0.8 4,967,885 + AO356_22190 0.41 +0.8 4,967,887 + AO356_22190 0.42 +0.2 4,968,037 + AO356_22190 0.82 -0.3
Or see this region's nucleotide sequence