Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_21830 and AO356_21835 are separated by 347 nucleotides AO356_21835 and AO356_21840 are separated by 175 nucleotides AO356_21840 and AO356_21845 are separated by 224 nucleotides AO356_21845 and AO356_21850 overlap by 1 nucleotides
AO356_21830: AO356_21830 - hypothetical protein, at 4,888,091 to 4,888,372
_21830
AO356_21835: AO356_21835 - glyoxalase, at 4,888,720 to 4,889,103
_21835
AO356_21840: AO356_21840 - N-methylproline demethylase, at 4,889,279 to 4,891,315
_21840
AO356_21845: AO356_21845 - electron transporter RnfB, at 4,891,540 to 4,892,289
_21845
AO356_21850: AO356_21850 - electron transfer flavoprotein subunit beta, at 4,892,289 to 4,893,224
_21850
Position (kb)
4889
4890
4891
4892 Strain fitness (log2 ratio)
-2
-1
0
1 at 4888.370 kb on + strand at 4888.370 kb on + strand at 4888.616 kb on + strand at 4888.616 kb on + strand at 4888.722 kb on - strand at 4888.722 kb on - strand at 4888.739 kb on + strand at 4888.739 kb on + strand at 4888.739 kb on + strand at 4888.739 kb on + strand at 4888.776 kb on - strand, within AO356_21835 at 4889.043 kb on - strand, within AO356_21835 at 4889.092 kb on - strand at 4889.119 kb on - strand at 4889.165 kb on + strand at 4889.310 kb on + strand at 4889.311 kb on - strand at 4889.311 kb on - strand at 4889.311 kb on - strand at 4889.314 kb on + strand at 4889.315 kb on - strand at 4889.326 kb on + strand at 4889.327 kb on - strand at 4889.327 kb on - strand at 4889.329 kb on + strand at 4889.330 kb on - strand at 4889.460 kb on - strand at 4889.460 kb on - strand at 4889.470 kb on - strand at 4889.545 kb on - strand, within AO356_21840 at 4889.604 kb on + strand, within AO356_21840 at 4889.649 kb on + strand, within AO356_21840 at 4889.649 kb on + strand, within AO356_21840 at 4889.649 kb on + strand, within AO356_21840 at 4889.649 kb on + strand, within AO356_21840 at 4889.649 kb on + strand, within AO356_21840 at 4889.649 kb on + strand, within AO356_21840 at 4889.650 kb on - strand, within AO356_21840 at 4889.650 kb on - strand, within AO356_21840 at 4889.751 kb on - strand, within AO356_21840 at 4890.232 kb on - strand, within AO356_21840 at 4890.250 kb on - strand, within AO356_21840 at 4890.250 kb on - strand, within AO356_21840 at 4890.250 kb on - strand, within AO356_21840 at 4890.267 kb on + strand, within AO356_21840 at 4890.267 kb on + strand, within AO356_21840 at 4890.267 kb on + strand, within AO356_21840 at 4890.267 kb on + strand, within AO356_21840 at 4890.267 kb on + strand, within AO356_21840 at 4890.268 kb on - strand, within AO356_21840 at 4890.268 kb on - strand, within AO356_21840 at 4890.268 kb on - strand, within AO356_21840 at 4890.268 kb on - strand, within AO356_21840 at 4890.750 kb on + strand, within AO356_21840 at 4890.867 kb on + strand, within AO356_21840 at 4890.868 kb on - strand, within AO356_21840 at 4891.087 kb on - strand, within AO356_21840 at 4891.087 kb on - strand, within AO356_21840 at 4891.188 kb on + strand at 4891.189 kb on - strand at 4891.285 kb on + strand at 4891.285 kb on + strand at 4891.286 kb on - strand at 4891.287 kb on + strand at 4891.287 kb on + strand at 4891.287 kb on + strand at 4891.287 kb on + strand at 4891.440 kb on + strand at 4891.441 kb on - strand at 4891.441 kb on - strand at 4891.527 kb on + strand at 4891.838 kb on - strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.963 kb on + strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4891.964 kb on - strand, within AO356_21845 at 4892.093 kb on - strand, within AO356_21845 at 4892.189 kb on + strand, within AO356_21845 at 4892.190 kb on - strand, within AO356_21845 at 4892.192 kb on + strand, within AO356_21845 at 4892.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,888,370 + -0.2 4,888,370 + -1.5 4,888,616 + +0.4 4,888,616 + -0.6 4,888,722 - -0.7 4,888,722 - -0.7 4,888,739 + +0.6 4,888,739 + -0.2 4,888,739 + -0.1 4,888,739 + -0.3 4,888,776 - AO356_21835 0.15 -0.5 4,889,043 - AO356_21835 0.84 -1.5 4,889,092 - -0.4 4,889,119 - +0.3 4,889,165 + +0.1 4,889,310 + +0.6 4,889,311 - -0.1 4,889,311 - +0.7 4,889,311 - +0.8 4,889,314 + +0.6 4,889,315 - -0.0 4,889,326 + +0.3 4,889,327 - +0.2 4,889,327 - -1.3 4,889,329 + +0.1 4,889,330 - +0.6 4,889,460 - -0.8 4,889,460 - -2.7 4,889,470 - -0.5 4,889,545 - AO356_21840 0.13 -0.5 4,889,604 + AO356_21840 0.16 +0.4 4,889,649 + AO356_21840 0.18 -0.0 4,889,649 + AO356_21840 0.18 +0.4 4,889,649 + AO356_21840 0.18 -0.2 4,889,649 + AO356_21840 0.18 +0.4 4,889,649 + AO356_21840 0.18 +0.2 4,889,649 + AO356_21840 0.18 -0.1 4,889,650 - AO356_21840 0.18 +1.1 4,889,650 - AO356_21840 0.18 +1.2 4,889,751 - AO356_21840 0.23 +0.1 4,890,232 - AO356_21840 0.47 -0.0 4,890,250 - AO356_21840 0.48 +0.1 4,890,250 - AO356_21840 0.48 +1.0 4,890,250 - AO356_21840 0.48 -0.6 4,890,267 + AO356_21840 0.49 -1.9 4,890,267 + AO356_21840 0.49 +0.7 4,890,267 + AO356_21840 0.49 +0.4 4,890,267 + AO356_21840 0.49 +0.2 4,890,267 + AO356_21840 0.49 +0.2 4,890,268 - AO356_21840 0.49 -0.3 4,890,268 - AO356_21840 0.49 -0.3 4,890,268 - AO356_21840 0.49 -0.4 4,890,268 - AO356_21840 0.49 -0.7 4,890,750 + AO356_21840 0.72 +0.1 4,890,867 + AO356_21840 0.78 +0.6 4,890,868 - AO356_21840 0.78 -0.1 4,891,087 - AO356_21840 0.89 -0.1 4,891,087 - AO356_21840 0.89 -0.0 4,891,188 + -0.2 4,891,189 - +1.0 4,891,285 + -0.2 4,891,285 + -0.3 4,891,286 - +0.4 4,891,287 + +0.4 4,891,287 + -1.8 4,891,287 + +0.2 4,891,287 + -0.8 4,891,440 + +0.3 4,891,441 - -1.0 4,891,441 - +0.4 4,891,527 + +0.2 4,891,838 - AO356_21845 0.40 +0.1 4,891,963 + AO356_21845 0.56 -0.7 4,891,963 + AO356_21845 0.56 -0.9 4,891,963 + AO356_21845 0.56 +0.5 4,891,963 + AO356_21845 0.56 -0.1 4,891,963 + AO356_21845 0.56 -0.7 4,891,963 + AO356_21845 0.56 -0.7 4,891,963 + AO356_21845 0.56 -0.4 4,891,964 - AO356_21845 0.57 +0.1 4,891,964 - AO356_21845 0.57 +0.6 4,891,964 - AO356_21845 0.57 -0.5 4,891,964 - AO356_21845 0.57 +0.1 4,891,964 - AO356_21845 0.57 -0.0 4,892,093 - AO356_21845 0.74 -1.2 4,892,189 + AO356_21845 0.87 -0.3 4,892,190 - AO356_21845 0.87 +0.3 4,892,192 + AO356_21845 0.87 +0.3 4,892,260 - +0.1
Or see this region's nucleotide sequence