Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_21065 and AO356_21070 are separated by 473 nucleotides AO356_21070 and AO356_21075 are separated by 121 nucleotides AO356_21075 and AO356_21080 overlap by 8 nucleotides
AO356_21065: AO356_21065 - hypothetical protein, at 4,685,078 to 4,688,668
_21065
AO356_21070: AO356_21070 - hypothetical protein, at 4,689,142 to 4,689,498
_21070
AO356_21075: AO356_21075 - glucoamylase, at 4,689,620 to 4,691,443
_21075
AO356_21080: AO356_21080 - sugar dehydrogenase, at 4,691,436 to 4,692,236
_21080
Position (kb)
4689
4690
4691
4692 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 4688.621 kb on + strand at 4688.621 kb on + strand at 4688.621 kb on + strand at 4688.621 kb on + strand at 4688.621 kb on + strand at 4688.622 kb on - strand at 4688.622 kb on - strand at 4688.630 kb on + strand at 4688.631 kb on - strand at 4688.631 kb on - strand at 4688.631 kb on - strand at 4688.666 kb on + strand at 4688.667 kb on - strand at 4688.779 kb on + strand at 4688.782 kb on - strand at 4689.077 kb on + strand at 4689.077 kb on + strand at 4689.077 kb on + strand at 4689.078 kb on - strand at 4689.080 kb on - strand at 4689.921 kb on + strand, within AO356_21075 at 4690.107 kb on + strand, within AO356_21075 at 4690.107 kb on + strand, within AO356_21075 at 4690.108 kb on - strand, within AO356_21075 at 4690.108 kb on - strand, within AO356_21075 at 4690.342 kb on - strand, within AO356_21075 at 4690.342 kb on - strand, within AO356_21075 at 4690.404 kb on + strand, within AO356_21075 at 4690.564 kb on + strand, within AO356_21075 at 4690.565 kb on - strand, within AO356_21075 at 4690.565 kb on - strand, within AO356_21075 at 4690.566 kb on + strand, within AO356_21075 at 4690.566 kb on + strand, within AO356_21075 at 4690.566 kb on + strand, within AO356_21075 at 4690.567 kb on - strand, within AO356_21075 at 4690.567 kb on - strand, within AO356_21075 at 4690.913 kb on - strand, within AO356_21075 at 4690.913 kb on - strand, within AO356_21075 at 4690.913 kb on - strand, within AO356_21075 at 4691.050 kb on - strand, within AO356_21075 at 4691.226 kb on + strand, within AO356_21075 at 4691.227 kb on - strand, within AO356_21075 at 4691.227 kb on - strand, within AO356_21075 at 4691.227 kb on - strand, within AO356_21075 at 4691.506 kb on + strand at 4691.506 kb on + strand at 4691.743 kb on + strand, within AO356_21080 at 4691.743 kb on + strand, within AO356_21080 at 4692.124 kb on + strand, within AO356_21080 at 4692.124 kb on + strand, within AO356_21080 at 4692.125 kb on - strand, within AO356_21080 at 4692.125 kb on - strand, within AO356_21080 at 4692.228 kb on + strand at 4692.263 kb on + strand at 4692.263 kb on + strand at 4692.263 kb on + strand at 4692.264 kb on - strand at 4692.317 kb on + strand at 4692.364 kb on + strand at 4692.364 kb on + strand at 4692.364 kb on + strand at 4692.364 kb on + strand at 4692.364 kb on + strand at 4692.364 kb on + strand at 4692.364 kb on + strand at 4692.364 kb on + strand at 4692.364 kb on + strand at 4692.365 kb on - strand at 4692.365 kb on - strand at 4692.365 kb on - strand at 4692.365 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,688,621 + +0.5 4,688,621 + -0.3 4,688,621 + +4.6 4,688,621 + -0.1 4,688,621 + +0.8 4,688,622 - +1.4 4,688,622 - -0.1 4,688,630 + +0.5 4,688,631 - +0.2 4,688,631 - +0.4 4,688,631 - +0.4 4,688,666 + +0.2 4,688,667 - +0.1 4,688,779 + -0.7 4,688,782 - +1.3 4,689,077 + +1.0 4,689,077 + +0.6 4,689,077 + +1.7 4,689,078 - +0.7 4,689,080 - +0.2 4,689,921 + AO356_21075 0.17 -0.4 4,690,107 + AO356_21075 0.27 -0.3 4,690,107 + AO356_21075 0.27 +1.1 4,690,108 - AO356_21075 0.27 -0.7 4,690,108 - AO356_21075 0.27 -0.3 4,690,342 - AO356_21075 0.40 -0.1 4,690,342 - AO356_21075 0.40 +0.0 4,690,404 + AO356_21075 0.43 -0.6 4,690,564 + AO356_21075 0.52 +0.2 4,690,565 - AO356_21075 0.52 -0.6 4,690,565 - AO356_21075 0.52 -0.2 4,690,566 + AO356_21075 0.52 +0.4 4,690,566 + AO356_21075 0.52 +0.2 4,690,566 + AO356_21075 0.52 -0.4 4,690,567 - AO356_21075 0.52 +0.4 4,690,567 - AO356_21075 0.52 +0.6 4,690,913 - AO356_21075 0.71 -0.4 4,690,913 - AO356_21075 0.71 +0.7 4,690,913 - AO356_21075 0.71 +0.3 4,691,050 - AO356_21075 0.78 +0.0 4,691,226 + AO356_21075 0.88 -0.5 4,691,227 - AO356_21075 0.88 +0.1 4,691,227 - AO356_21075 0.88 +0.9 4,691,227 - AO356_21075 0.88 -0.8 4,691,506 + -0.3 4,691,506 + -0.0 4,691,743 + AO356_21080 0.38 +0.4 4,691,743 + AO356_21080 0.38 +0.9 4,692,124 + AO356_21080 0.86 +1.2 4,692,124 + AO356_21080 0.86 +0.6 4,692,125 - AO356_21080 0.86 -0.1 4,692,125 - AO356_21080 0.86 +0.7 4,692,228 + -0.1 4,692,263 + +0.2 4,692,263 + -0.2 4,692,263 + -0.4 4,692,264 - +1.0 4,692,317 + -0.8 4,692,364 + -1.7 4,692,364 + +0.8 4,692,364 + +0.6 4,692,364 + -0.6 4,692,364 + -0.3 4,692,364 + -0.9 4,692,364 + -1.6 4,692,364 + -0.2 4,692,364 + +0.1 4,692,365 - -0.2 4,692,365 - +0.4 4,692,365 - -0.1 4,692,365 - +0.4
Or see this region's nucleotide sequence