Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_14970 and AO356_14975 overlap by 4 nucleotides AO356_14975 and AO356_14980 overlap by 1 nucleotides AO356_14980 and AO356_14985 are separated by 117 nucleotides
AO356_14970: AO356_14970 - GTP cyclohydrolase, at 3,384,712 to 3,385,329
_14970
AO356_14975: AO356_14975 - MFS transporter, at 3,385,326 to 3,385,745
_14975
AO356_14980: AO356_14980 - iron-hydroxamate ABC transporter substrate-binding protein, at 3,385,745 to 3,386,530
_14980
AO356_14985: AO356_14985 - TonB-dependent receptor, at 3,386,648 to 3,388,528
_14985
Position (kb)
3385
3386
3387 Strain fitness (log2 ratio)
-1
0
1 at 3385.375 kb on - strand, within AO356_14975 at 3385.375 kb on - strand, within AO356_14975 at 3385.516 kb on - strand, within AO356_14975 at 3385.516 kb on - strand, within AO356_14975 at 3385.517 kb on + strand, within AO356_14975 at 3385.517 kb on + strand, within AO356_14975 at 3385.517 kb on + strand, within AO356_14975 at 3385.517 kb on + strand, within AO356_14975 at 3385.517 kb on + strand, within AO356_14975 at 3385.518 kb on - strand, within AO356_14975 at 3385.518 kb on - strand, within AO356_14975 at 3385.713 kb on + strand at 3385.713 kb on + strand at 3385.713 kb on + strand at 3385.713 kb on + strand at 3385.713 kb on + strand at 3385.714 kb on - strand at 3386.136 kb on + strand, within AO356_14980 at 3386.136 kb on + strand, within AO356_14980 at 3386.136 kb on + strand, within AO356_14980 at 3386.137 kb on - strand, within AO356_14980 at 3386.216 kb on + strand, within AO356_14980 at 3386.217 kb on - strand, within AO356_14980 at 3386.217 kb on - strand, within AO356_14980 at 3386.240 kb on + strand, within AO356_14980 at 3386.241 kb on - strand, within AO356_14980 at 3386.241 kb on - strand, within AO356_14980 at 3386.359 kb on + strand, within AO356_14980 at 3386.359 kb on + strand, within AO356_14980 at 3386.360 kb on - strand, within AO356_14980 at 3386.360 kb on - strand, within AO356_14980 at 3386.502 kb on + strand at 3386.502 kb on + strand at 3386.503 kb on - strand at 3386.545 kb on - strand at 3386.545 kb on - strand at 3386.709 kb on + strand at 3386.709 kb on + strand at 3386.709 kb on + strand at 3386.710 kb on - strand at 3386.710 kb on - strand at 3386.710 kb on - strand at 3386.728 kb on + strand at 3386.728 kb on + strand at 3386.728 kb on + strand at 3386.730 kb on + strand at 3386.730 kb on + strand at 3386.731 kb on - strand at 3386.731 kb on - strand at 3386.745 kb on + strand at 3386.746 kb on - strand at 3386.868 kb on + strand, within AO356_14985 at 3386.868 kb on + strand, within AO356_14985 at 3386.869 kb on - strand, within AO356_14985 at 3386.869 kb on - strand, within AO356_14985 at 3386.869 kb on - strand, within AO356_14985 at 3386.869 kb on - strand, within AO356_14985 at 3386.869 kb on - strand, within AO356_14985 at 3387.045 kb on + strand, within AO356_14985 at 3387.045 kb on + strand, within AO356_14985 at 3387.046 kb on - strand, within AO356_14985 at 3387.196 kb on - strand, within AO356_14985 at 3387.196 kb on - strand, within AO356_14985 at 3387.244 kb on - strand, within AO356_14985 at 3387.246 kb on + strand, within AO356_14985 at 3387.246 kb on + strand, within AO356_14985 at 3387.246 kb on + strand, within AO356_14985 at 3387.247 kb on - strand, within AO356_14985 at 3387.247 kb on - strand, within AO356_14985 at 3387.247 kb on - strand, within AO356_14985 at 3387.247 kb on - strand, within AO356_14985 at 3387.247 kb on - strand, within AO356_14985 at 3387.247 kb on - strand, within AO356_14985 at 3387.285 kb on + strand, within AO356_14985 at 3387.285 kb on + strand, within AO356_14985 at 3387.285 kb on + strand, within AO356_14985 at 3387.285 kb on + strand, within AO356_14985 at 3387.286 kb on - strand, within AO356_14985 at 3387.286 kb on - strand, within AO356_14985 at 3387.286 kb on - strand, within AO356_14985 at 3387.493 kb on - strand, within AO356_14985
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,385,375 - AO356_14975 0.12 +0.8 3,385,375 - AO356_14975 0.12 +0.2 3,385,516 - AO356_14975 0.45 -0.6 3,385,516 - AO356_14975 0.45 +0.1 3,385,517 + AO356_14975 0.45 -0.6 3,385,517 + AO356_14975 0.45 +1.0 3,385,517 + AO356_14975 0.45 -0.6 3,385,517 + AO356_14975 0.45 -0.8 3,385,517 + AO356_14975 0.45 +0.7 3,385,518 - AO356_14975 0.46 -0.5 3,385,518 - AO356_14975 0.46 -1.3 3,385,713 + +0.1 3,385,713 + -0.9 3,385,713 + -1.2 3,385,713 + -0.1 3,385,713 + +0.3 3,385,714 - +0.5 3,386,136 + AO356_14980 0.50 -0.4 3,386,136 + AO356_14980 0.50 -0.3 3,386,136 + AO356_14980 0.50 +0.0 3,386,137 - AO356_14980 0.50 +0.2 3,386,216 + AO356_14980 0.60 +1.0 3,386,217 - AO356_14980 0.60 -0.6 3,386,217 - AO356_14980 0.60 -1.0 3,386,240 + AO356_14980 0.63 +0.1 3,386,241 - AO356_14980 0.63 -0.0 3,386,241 - AO356_14980 0.63 +0.3 3,386,359 + AO356_14980 0.78 +0.5 3,386,359 + AO356_14980 0.78 +0.7 3,386,360 - AO356_14980 0.78 +0.6 3,386,360 - AO356_14980 0.78 +0.7 3,386,502 + -0.5 3,386,502 + -1.4 3,386,503 - +0.0 3,386,545 - +0.7 3,386,545 - +0.0 3,386,709 + +0.3 3,386,709 + -0.8 3,386,709 + +0.6 3,386,710 - +0.7 3,386,710 - +0.4 3,386,710 - -0.4 3,386,728 + +0.6 3,386,728 + +0.2 3,386,728 + +0.2 3,386,730 + -0.2 3,386,730 + +0.7 3,386,731 - +0.4 3,386,731 - -0.1 3,386,745 + -0.2 3,386,746 - +1.0 3,386,868 + AO356_14985 0.12 -0.4 3,386,868 + AO356_14985 0.12 +0.8 3,386,869 - AO356_14985 0.12 +0.3 3,386,869 - AO356_14985 0.12 +0.9 3,386,869 - AO356_14985 0.12 +0.4 3,386,869 - AO356_14985 0.12 +0.1 3,386,869 - AO356_14985 0.12 +0.6 3,387,045 + AO356_14985 0.21 +0.3 3,387,045 + AO356_14985 0.21 -0.6 3,387,046 - AO356_14985 0.21 +1.6 3,387,196 - AO356_14985 0.29 +0.1 3,387,196 - AO356_14985 0.29 +0.4 3,387,244 - AO356_14985 0.32 +0.1 3,387,246 + AO356_14985 0.32 -0.4 3,387,246 + AO356_14985 0.32 -0.0 3,387,246 + AO356_14985 0.32 +0.5 3,387,247 - AO356_14985 0.32 -0.2 3,387,247 - AO356_14985 0.32 +0.0 3,387,247 - AO356_14985 0.32 -0.5 3,387,247 - AO356_14985 0.32 +0.1 3,387,247 - AO356_14985 0.32 -0.4 3,387,247 - AO356_14985 0.32 +0.1 3,387,285 + AO356_14985 0.34 +0.3 3,387,285 + AO356_14985 0.34 +0.0 3,387,285 + AO356_14985 0.34 -0.2 3,387,285 + AO356_14985 0.34 +0.8 3,387,286 - AO356_14985 0.34 +0.4 3,387,286 - AO356_14985 0.34 -0.7 3,387,286 - AO356_14985 0.34 +0.1 3,387,493 - AO356_14985 0.45 +0.3
Or see this region's nucleotide sequence