Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_04225 and AO356_04230 are separated by 150 nucleotides AO356_04230 and AO356_04235 are separated by 50 nucleotides AO356_04235 and AO356_04240 are separated by 14 nucleotides AO356_04240 and AO356_04245 are separated by 4 nucleotides
AO356_04225: AO356_04225 - FMN-dependent NADH-azoreductase, at 960,183 to 960,782
_04225
AO356_04230: AO356_04230 - 3-phosphoglycerate kinase, at 960,933 to 961,247
_04230
AO356_04235: AO356_04235 - LysR family transcriptional regulator, at 961,298 to 962,221
_04235
AO356_04240: AO356_04240 - 3-oxoadipate enol-lactonase, at 962,236 to 963,039
_04240
AO356_04245: AO356_04245 - peptidylprolyl isomerase, at 963,044 to 963,607
_04245
Position (kb) 961
962
963 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 960.316 kb on + strand, within AO356_04225 at 960.316 kb on + strand, within AO356_04225 at 960.421 kb on + strand, within AO356_04225 at 960.422 kb on - strand, within AO356_04225 at 960.422 kb on - strand, within AO356_04225 at 960.422 kb on - strand, within AO356_04225 at 960.422 kb on - strand, within AO356_04225 at 960.493 kb on + strand, within AO356_04225 at 960.493 kb on + strand, within AO356_04225 at 960.494 kb on - strand, within AO356_04225 at 960.494 kb on - strand, within AO356_04225 at 960.494 kb on - strand, within AO356_04225 at 960.789 kb on + strand at 960.790 kb on - strand at 960.790 kb on - strand at 960.790 kb on - strand at 960.831 kb on + strand at 960.832 kb on - strand at 960.864 kb on - strand at 961.096 kb on + strand, within AO356_04230 at 961.096 kb on + strand, within AO356_04230 at 961.096 kb on + strand, within AO356_04230 at 961.097 kb on - strand, within AO356_04230 at 961.139 kb on - strand, within AO356_04230 at 961.147 kb on + strand, within AO356_04230 at 961.148 kb on - strand, within AO356_04230 at 961.279 kb on - strand at 961.279 kb on - strand at 961.279 kb on - strand at 961.412 kb on + strand, within AO356_04235 at 961.412 kb on + strand, within AO356_04235 at 962.064 kb on + strand, within AO356_04235 at 962.065 kb on - strand, within AO356_04235 at 962.102 kb on + strand, within AO356_04235 at 962.102 kb on + strand, within AO356_04235 at 962.102 kb on + strand, within AO356_04235 at 962.103 kb on - strand, within AO356_04235 at 962.103 kb on - strand, within AO356_04235 at 962.242 kb on + strand at 962.242 kb on + strand at 962.243 kb on - strand at 962.431 kb on + strand, within AO356_04240 at 962.676 kb on + strand, within AO356_04240 at 962.730 kb on - strand, within AO356_04240 at 962.776 kb on + strand, within AO356_04240 at 962.776 kb on + strand, within AO356_04240 at 962.776 kb on + strand, within AO356_04240 at 962.776 kb on + strand, within AO356_04240 at 962.777 kb on - strand, within AO356_04240 at 962.777 kb on - strand, within AO356_04240 at 962.873 kb on - strand, within AO356_04240 at 962.873 kb on - strand, within AO356_04240 at 962.873 kb on - strand, within AO356_04240 at 962.873 kb on - strand, within AO356_04240 at 962.899 kb on + strand, within AO356_04240 at 962.900 kb on - strand, within AO356_04240 at 962.900 kb on - strand, within AO356_04240 at 962.903 kb on + strand, within AO356_04240 at 962.903 kb on + strand, within AO356_04240 at 962.904 kb on - strand, within AO356_04240 at 962.904 kb on - strand, within AO356_04240 at 962.904 kb on - strand, within AO356_04240 at 962.904 kb on - strand, within AO356_04240 at 962.904 kb on - strand, within AO356_04240 at 962.904 kb on - strand, within AO356_04240 at 962.904 kb on - strand, within AO356_04240 at 963.129 kb on + strand, within AO356_04245 at 963.129 kb on + strand, within AO356_04245 at 963.129 kb on + strand, within AO356_04245 at 963.129 kb on + strand, within AO356_04245 at 963.129 kb on + strand, within AO356_04245 at 963.129 kb on + strand, within AO356_04245 at 963.129 kb on + strand, within AO356_04245 at 963.129 kb on + strand, within AO356_04245 at 963.129 kb on + strand, within AO356_04245 at 963.129 kb on + strand, within AO356_04245 at 963.130 kb on - strand, within AO356_04245 at 963.130 kb on - strand, within AO356_04245 at 963.130 kb on - strand, within AO356_04245 at 963.130 kb on - strand, within AO356_04245 at 963.130 kb on - strand, within AO356_04245 at 963.194 kb on - strand, within AO356_04245 at 963.212 kb on + strand, within AO356_04245 at 963.212 kb on + strand, within AO356_04245 at 963.213 kb on - strand, within AO356_04245 at 963.213 kb on - strand, within AO356_04245
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 960,316 + AO356_04225 0.22 -0.1 960,316 + AO356_04225 0.22 -0.9 960,421 + AO356_04225 0.40 -0.9 960,422 - AO356_04225 0.40 -1.4 960,422 - AO356_04225 0.40 +2.7 960,422 - AO356_04225 0.40 +1.6 960,422 - AO356_04225 0.40 +0.3 960,493 + AO356_04225 0.52 -0.8 960,493 + AO356_04225 0.52 -1.2 960,494 - AO356_04225 0.52 +0.1 960,494 - AO356_04225 0.52 -0.5 960,494 - AO356_04225 0.52 +0.6 960,789 + -0.3 960,790 - -0.4 960,790 - -1.4 960,790 - -0.4 960,831 + +0.4 960,832 - -0.5 960,864 - -0.1 961,096 + AO356_04230 0.52 -0.1 961,096 + AO356_04230 0.52 +0.7 961,096 + AO356_04230 0.52 -0.4 961,097 - AO356_04230 0.52 +0.1 961,139 - AO356_04230 0.65 -0.3 961,147 + AO356_04230 0.68 -0.1 961,148 - AO356_04230 0.68 -0.7 961,279 - -0.2 961,279 - +0.1 961,279 - +0.5 961,412 + AO356_04235 0.12 -1.3 961,412 + AO356_04235 0.12 +0.7 962,064 + AO356_04235 0.83 -1.2 962,065 - AO356_04235 0.83 -0.8 962,102 + AO356_04235 0.87 -1.2 962,102 + AO356_04235 0.87 -1.6 962,102 + AO356_04235 0.87 +0.8 962,103 - AO356_04235 0.87 -0.9 962,103 - AO356_04235 0.87 -3.4 962,242 + -0.4 962,242 + +1.1 962,243 - +0.5 962,431 + AO356_04240 0.24 -0.9 962,676 + AO356_04240 0.55 +0.6 962,730 - AO356_04240 0.61 -0.3 962,776 + AO356_04240 0.67 -1.6 962,776 + AO356_04240 0.67 +0.7 962,776 + AO356_04240 0.67 -0.3 962,776 + AO356_04240 0.67 -1.0 962,777 - AO356_04240 0.67 -0.5 962,777 - AO356_04240 0.67 -0.2 962,873 - AO356_04240 0.79 +0.4 962,873 - AO356_04240 0.79 -0.8 962,873 - AO356_04240 0.79 +0.3 962,873 - AO356_04240 0.79 +0.4 962,899 + AO356_04240 0.82 -0.3 962,900 - AO356_04240 0.83 +0.6 962,900 - AO356_04240 0.83 +0.3 962,903 + AO356_04240 0.83 +0.3 962,903 + AO356_04240 0.83 -1.4 962,904 - AO356_04240 0.83 -0.3 962,904 - AO356_04240 0.83 -1.1 962,904 - AO356_04240 0.83 -0.8 962,904 - AO356_04240 0.83 +0.7 962,904 - AO356_04240 0.83 +0.9 962,904 - AO356_04240 0.83 -0.9 962,904 - AO356_04240 0.83 +0.5 963,129 + AO356_04245 0.15 -0.3 963,129 + AO356_04245 0.15 +0.2 963,129 + AO356_04245 0.15 -0.2 963,129 + AO356_04245 0.15 +0.5 963,129 + AO356_04245 0.15 -0.4 963,129 + AO356_04245 0.15 +0.0 963,129 + AO356_04245 0.15 +0.3 963,129 + AO356_04245 0.15 -0.4 963,129 + AO356_04245 0.15 -0.1 963,129 + AO356_04245 0.15 -0.3 963,130 - AO356_04245 0.15 +0.0 963,130 - AO356_04245 0.15 +1.2 963,130 - AO356_04245 0.15 +0.1 963,130 - AO356_04245 0.15 +0.4 963,130 - AO356_04245 0.15 -0.4 963,194 - AO356_04245 0.27 -0.3 963,212 + AO356_04245 0.30 -0.3 963,212 + AO356_04245 0.30 +0.3 963,213 - AO356_04245 0.30 +1.0 963,213 - AO356_04245 0.30 +0.8
Or see this region's nucleotide sequence