Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_04225 and AO356_04230 are separated by 150 nucleotides AO356_04230 and AO356_04235 are separated by 50 nucleotides AO356_04235 and AO356_04240 are separated by 14 nucleotides
AO356_04225: AO356_04225 - FMN-dependent NADH-azoreductase, at 960,183 to 960,782
_04225
AO356_04230: AO356_04230 - 3-phosphoglycerate kinase, at 960,933 to 961,247
_04230
AO356_04235: AO356_04235 - LysR family transcriptional regulator, at 961,298 to 962,221
_04235
AO356_04240: AO356_04240 - 3-oxoadipate enol-lactonase, at 962,236 to 963,039
_04240
Position (kb) 960
961
962 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 959.951 kb on + strand at 959.961 kb on - strand at 959.961 kb on - strand at 959.961 kb on - strand at 959.974 kb on + strand at 960.026 kb on - strand at 960.093 kb on + strand at 960.093 kb on + strand at 960.093 kb on + strand at 960.093 kb on + strand at 960.093 kb on + strand at 960.093 kb on + strand at 960.093 kb on + strand at 960.093 kb on + strand at 960.093 kb on + strand at 960.093 kb on + strand at 960.093 kb on + strand at 960.093 kb on + strand at 960.094 kb on - strand at 960.094 kb on - strand at 960.094 kb on - strand at 960.094 kb on - strand at 960.094 kb on - strand at 960.094 kb on - strand at 960.094 kb on - strand at 960.149 kb on + strand at 960.150 kb on - strand at 960.150 kb on - strand at 960.150 kb on - strand at 960.316 kb on + strand, within AO356_04225 at 960.316 kb on + strand, within AO356_04225 at 960.421 kb on + strand, within AO356_04225 at 960.422 kb on - strand, within AO356_04225 at 960.422 kb on - strand, within AO356_04225 at 960.422 kb on - strand, within AO356_04225 at 960.422 kb on - strand, within AO356_04225 at 960.493 kb on + strand, within AO356_04225 at 960.493 kb on + strand, within AO356_04225 at 960.494 kb on - strand, within AO356_04225 at 960.494 kb on - strand, within AO356_04225 at 960.494 kb on - strand, within AO356_04225 at 960.789 kb on + strand at 960.790 kb on - strand at 960.790 kb on - strand at 960.790 kb on - strand at 960.831 kb on + strand at 960.832 kb on - strand at 960.864 kb on - strand at 961.096 kb on + strand, within AO356_04230 at 961.096 kb on + strand, within AO356_04230 at 961.096 kb on + strand, within AO356_04230 at 961.097 kb on - strand, within AO356_04230 at 961.139 kb on - strand, within AO356_04230 at 961.147 kb on + strand, within AO356_04230 at 961.148 kb on - strand, within AO356_04230 at 961.279 kb on - strand at 961.279 kb on - strand at 961.279 kb on - strand at 961.412 kb on + strand, within AO356_04235 at 961.412 kb on + strand, within AO356_04235 at 962.064 kb on + strand, within AO356_04235 at 962.065 kb on - strand, within AO356_04235 at 962.102 kb on + strand, within AO356_04235 at 962.102 kb on + strand, within AO356_04235 at 962.102 kb on + strand, within AO356_04235 at 962.103 kb on - strand, within AO356_04235 at 962.103 kb on - strand, within AO356_04235 at 962.242 kb on + strand at 962.242 kb on + strand at 962.243 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 959,951 + -1.1 959,961 - +0.9 959,961 - -0.1 959,961 - +0.4 959,974 + -1.3 960,026 - +0.2 960,093 + +0.4 960,093 + -0.1 960,093 + +0.0 960,093 + -1.4 960,093 + -0.1 960,093 + -2.1 960,093 + -0.2 960,093 + -0.8 960,093 + -0.8 960,093 + -0.3 960,093 + -0.2 960,093 + +0.2 960,094 - -0.2 960,094 - +0.5 960,094 - -1.3 960,094 - +1.0 960,094 - -0.5 960,094 - +0.6 960,094 - +0.0 960,149 + -0.2 960,150 - -0.8 960,150 - +0.5 960,150 - -1.0 960,316 + AO356_04225 0.22 -0.1 960,316 + AO356_04225 0.22 -0.9 960,421 + AO356_04225 0.40 -0.9 960,422 - AO356_04225 0.40 -1.4 960,422 - AO356_04225 0.40 +2.7 960,422 - AO356_04225 0.40 +1.6 960,422 - AO356_04225 0.40 +0.3 960,493 + AO356_04225 0.52 -0.8 960,493 + AO356_04225 0.52 -1.2 960,494 - AO356_04225 0.52 +0.1 960,494 - AO356_04225 0.52 -0.5 960,494 - AO356_04225 0.52 +0.6 960,789 + -0.3 960,790 - -0.4 960,790 - -1.4 960,790 - -0.4 960,831 + +0.4 960,832 - -0.5 960,864 - -0.1 961,096 + AO356_04230 0.52 -0.1 961,096 + AO356_04230 0.52 +0.7 961,096 + AO356_04230 0.52 -0.4 961,097 - AO356_04230 0.52 +0.1 961,139 - AO356_04230 0.65 -0.3 961,147 + AO356_04230 0.68 -0.1 961,148 - AO356_04230 0.68 -0.7 961,279 - -0.2 961,279 - +0.1 961,279 - +0.5 961,412 + AO356_04235 0.12 -1.3 961,412 + AO356_04235 0.12 +0.7 962,064 + AO356_04235 0.83 -1.2 962,065 - AO356_04235 0.83 -0.8 962,102 + AO356_04235 0.87 -1.2 962,102 + AO356_04235 0.87 -1.6 962,102 + AO356_04235 0.87 +0.8 962,103 - AO356_04235 0.87 -0.9 962,103 - AO356_04235 0.87 -3.4 962,242 + -0.4 962,242 + +1.1 962,243 - +0.5
Or see this region's nucleotide sequence