Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02205 and AO356_02210 are separated by 61 nucleotides AO356_02210 and AO356_02215 are separated by 152 nucleotides AO356_02215 and AO356_02220 overlap by 4 nucleotides
AO356_02205: AO356_02205 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 473,490 to 474,320
_02205
AO356_02210: AO356_02210 - copper-binding protein, at 474,382 to 474,903
_02210
AO356_02215: AO356_02215 - transcriptional regulator, at 475,056 to 475,736
_02215
AO356_02220: AO356_02220 - ATPase, at 475,733 to 477,085
_02220
Position (kb) 474
475 Strain fitness (log2 ratio) -2
-1
0
1 at 473.407 kb on + strand at 473.407 kb on + strand at 473.408 kb on - strand at 473.408 kb on - strand at 473.408 kb on - strand at 473.491 kb on + strand at 473.491 kb on + strand at 473.571 kb on + strand at 473.571 kb on + strand at 473.571 kb on + strand at 473.571 kb on + strand at 473.572 kb on - strand at 473.572 kb on - strand at 473.572 kb on - strand at 473.584 kb on + strand, within AO356_02205 at 473.584 kb on + strand, within AO356_02205 at 473.686 kb on + strand, within AO356_02205 at 473.687 kb on - strand, within AO356_02205 at 473.687 kb on - strand, within AO356_02205 at 473.857 kb on + strand, within AO356_02205 at 474.262 kb on + strand at 474.263 kb on - strand at 474.263 kb on - strand at 474.275 kb on - strand at 474.275 kb on - strand at 474.353 kb on + strand at 474.354 kb on - strand at 474.354 kb on - strand at 474.436 kb on - strand, within AO356_02210 at 474.436 kb on - strand, within AO356_02210 at 474.693 kb on + strand, within AO356_02210 at 474.732 kb on + strand, within AO356_02210 at 474.909 kb on + strand at 474.978 kb on + strand at 475.019 kb on + strand at 475.019 kb on + strand at 475.019 kb on + strand at 475.019 kb on + strand at 475.020 kb on - strand at 475.020 kb on - strand at 475.021 kb on + strand at 475.021 kb on + strand at 475.022 kb on - strand at 475.098 kb on + strand at 475.098 kb on + strand at 475.099 kb on - strand at 475.099 kb on - strand at 475.188 kb on + strand, within AO356_02215 at 475.188 kb on + strand, within AO356_02215 at 475.189 kb on - strand, within AO356_02215 at 475.347 kb on + strand, within AO356_02215 at 475.348 kb on - strand, within AO356_02215 at 475.348 kb on - strand, within AO356_02215 at 475.679 kb on + strand at 475.679 kb on + strand at 475.680 kb on - strand at 475.680 kb on - strand at 475.680 kb on - strand at 475.680 kb on - strand at 475.680 kb on - strand at 475.710 kb on + strand at 475.710 kb on + strand at 475.710 kb on + strand at 475.710 kb on + strand at 475.710 kb on + strand at 475.711 kb on - strand at 475.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 473,407 + +0.6 473,407 + -1.0 473,408 - +0.2 473,408 - -0.5 473,408 - +0.2 473,491 + -0.3 473,491 + -0.4 473,571 + -1.7 473,571 + -2.7 473,571 + -0.5 473,571 + -0.1 473,572 - -0.8 473,572 - -2.3 473,572 - -2.1 473,584 + AO356_02205 0.11 -1.0 473,584 + AO356_02205 0.11 -0.5 473,686 + AO356_02205 0.24 -0.1 473,687 - AO356_02205 0.24 -1.4 473,687 - AO356_02205 0.24 +0.1 473,857 + AO356_02205 0.44 -0.4 474,262 + -0.3 474,263 - +0.7 474,263 - -0.7 474,275 - +1.0 474,275 - +0.1 474,353 + +0.7 474,354 - -0.0 474,354 - +0.6 474,436 - AO356_02210 0.10 +0.1 474,436 - AO356_02210 0.10 +0.7 474,693 + AO356_02210 0.60 +0.3 474,732 + AO356_02210 0.67 +1.1 474,909 + -0.3 474,978 + +0.2 475,019 + -0.0 475,019 + +0.6 475,019 + -0.3 475,019 + +0.1 475,020 - -0.8 475,020 - +0.6 475,021 + +0.2 475,021 + +0.1 475,022 - -0.0 475,098 + +1.1 475,098 + -0.1 475,099 - -0.6 475,099 - -0.4 475,188 + AO356_02215 0.19 +0.4 475,188 + AO356_02215 0.19 +0.1 475,189 - AO356_02215 0.20 -0.2 475,347 + AO356_02215 0.43 -0.4 475,348 - AO356_02215 0.43 -0.0 475,348 - AO356_02215 0.43 +0.3 475,679 + -0.5 475,679 + +0.0 475,680 - +0.8 475,680 - -1.4 475,680 - +0.7 475,680 - -0.0 475,680 - +0.5 475,710 + -0.1 475,710 + +0.0 475,710 + +1.5 475,710 + -0.5 475,710 + -0.8 475,711 - +0.8 475,711 - -0.4
Or see this region's nucleotide sequence