Experiment: 2-Furfuraldehyde 0.0625 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sucC and Shew_1658 overlap by 1 nucleotides Shew_1658 and Shew_1659 are separated by 85 nucleotides Shew_1659 and Shew_1660 are separated by 220 nucleotides Shew_1660 and fur are separated by 90 nucleotides
Shew_1657: sucC - succinyl-CoA synthetase subunit beta (RefSeq), at 1,906,416 to 1,907,582
sucC
Shew_1658: Shew_1658 - succinyl-CoA synthetase, alpha subunit (RefSeq), at 1,907,582 to 1,908,454
_1658
Shew_1659: Shew_1659 - ferredoxin-dependent glutamate synthase (RefSeq), at 1,908,540 to 1,910,213
_1659
Shew_1660: Shew_1660 - GCN5-related N-acetyltransferase (RefSeq), at 1,910,434 to 1,910,934
_1660
Shew_1661: fur - ferric uptake regulator (RefSeq), at 1,911,025 to 1,911,456
fur
Position (kb) 1908
1909
1910
1911 Strain fitness (log2 ratio) -1
0
1 at 1909.085 kb on + strand, within Shew_1659 at 1909.585 kb on - strand, within Shew_1659 at 1909.792 kb on + strand, within Shew_1659 at 1910.042 kb on + strand, within Shew_1659 at 1910.097 kb on - strand at 1910.107 kb on - strand at 1910.640 kb on + strand, within Shew_1660 at 1910.926 kb on + strand at 1911.080 kb on - strand, within fur
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Furfuraldehyde 0.0625 vol% remove 1,909,085 + Shew_1659 0.33 +0.2 1,909,585 - Shew_1659 0.62 +0.2 1,909,792 + Shew_1659 0.75 +0.2 1,910,042 + Shew_1659 0.90 +0.1 1,910,097 - -0.2 1,910,107 - +0.9 1,910,640 + Shew_1660 0.41 -0.5 1,910,926 + +0.3 1,911,080 - fur Shew_1661 0.13 +1.7
Or see this region's nucleotide sequence