Experiment: L-Malic Acid (C) and N2; no light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2215 and Rru_A2216 are separated by 145 nucleotides Rru_A2216 and Rru_A2217 are separated by 129 nucleotides Rru_A2217 and Rru_A2218 are separated by 159 nucleotides
Rru_A2215: Rru_A2215 - Transcriptional Regulator, LysR family (NCBI), at 2,574,265 to 2,575,218
_A2215
Rru_A2216: Rru_A2216 - Protein of unknown function DUF1127 (NCBI), at 2,575,364 to 2,575,714
_A2216
Rru_A2217: Rru_A2217 - hypothetical protein (NCBI), at 2,575,844 to 2,576,596
_A2217
Rru_A2218: Rru_A2218 - Short-chain dehydrogenase/reductase SDR (NCBI), at 2,576,756 to 2,577,562
_A2218
Position (kb)
2575
2576
2577 Strain fitness (log2 ratio)
-2
-1
0
1 at 2575.013 kb on + strand, within Rru_A2215 at 2575.013 kb on + strand, within Rru_A2215 at 2575.013 kb on + strand, within Rru_A2215 at 2575.014 kb on - strand, within Rru_A2215 at 2575.014 kb on - strand, within Rru_A2215 at 2575.014 kb on - strand, within Rru_A2215 at 2575.150 kb on - strand at 2575.308 kb on - strand at 2575.308 kb on - strand at 2575.308 kb on - strand at 2575.442 kb on + strand, within Rru_A2216 at 2575.562 kb on + strand, within Rru_A2216 at 2575.563 kb on - strand, within Rru_A2216 at 2575.563 kb on - strand, within Rru_A2216 at 2575.812 kb on + strand at 2575.813 kb on - strand at 2575.813 kb on - strand at 2575.813 kb on - strand at 2575.813 kb on - strand at 2575.813 kb on - strand at 2576.491 kb on - strand, within Rru_A2217 at 2576.717 kb on + strand at 2576.717 kb on + strand at 2576.717 kb on + strand at 2576.717 kb on + strand at 2576.717 kb on + strand at 2576.717 kb on + strand at 2577.560 kb on + strand at 2577.560 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) and N2; no light remove 2,575,013 + Rru_A2215 0.78 +0.1 2,575,013 + Rru_A2215 0.78 +0.1 2,575,013 + Rru_A2215 0.78 +0.1 2,575,014 - Rru_A2215 0.79 +0.1 2,575,014 - Rru_A2215 0.79 +0.1 2,575,014 - Rru_A2215 0.79 +0.1 2,575,150 - -0.8 2,575,308 - -0.8 2,575,308 - -2.2 2,575,308 - +0.1 2,575,442 + Rru_A2216 0.22 -2.0 2,575,562 + Rru_A2216 0.56 +0.1 2,575,563 - Rru_A2216 0.57 +0.1 2,575,563 - Rru_A2216 0.57 +0.1 2,575,812 + -1.9 2,575,813 - +0.1 2,575,813 - +0.1 2,575,813 - -1.2 2,575,813 - +0.1 2,575,813 - -1.0 2,576,491 - Rru_A2217 0.86 +0.1 2,576,717 + -0.9 2,576,717 + -1.4 2,576,717 + +0.1 2,576,717 + -1.9 2,576,717 + -0.9 2,576,717 + -1.1 2,577,560 + -0.9 2,577,560 + -0.9
Or see this region's nucleotide sequence