Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_30115 and AO356_30120 are separated by 3 nucleotides AO356_30120 and AO356_30125 are separated by 591 nucleotides
AO356_30115: AO356_30115 - aminomethyltransferase, at 7,027,229 to 7,030,126
_30115
AO356_30120: AO356_30120 - sarcosine oxidase, at 7,030,130 to 7,030,714
_30120
AO356_30125: AO356_30125 - xylose isomerase, at 7,031,306 to 7,032,142
_30125
Position (kb) 7030
7031 Strain fitness (log2 ratio) -1
0
1 at 7029.206 kb on + strand, within AO356_30115 at 7029.206 kb on + strand, within AO356_30115 at 7029.206 kb on + strand, within AO356_30115 at 7029.207 kb on - strand, within AO356_30115 at 7029.207 kb on - strand, within AO356_30115 at 7029.324 kb on - strand, within AO356_30115 at 7029.596 kb on + strand, within AO356_30115 at 7029.596 kb on + strand, within AO356_30115 at 7030.086 kb on + strand at 7030.124 kb on + strand at 7030.124 kb on + strand at 7030.124 kb on + strand at 7030.253 kb on + strand, within AO356_30120 at 7030.275 kb on + strand, within AO356_30120 at 7030.349 kb on + strand, within AO356_30120 at 7030.433 kb on + strand, within AO356_30120 at 7030.433 kb on + strand, within AO356_30120 at 7030.434 kb on - strand, within AO356_30120 at 7030.434 kb on - strand, within AO356_30120 at 7030.732 kb on - strand at 7030.732 kb on - strand at 7030.779 kb on + strand at 7030.780 kb on - strand at 7030.780 kb on - strand at 7030.780 kb on - strand at 7030.780 kb on - strand at 7030.880 kb on + strand at 7031.012 kb on - strand at 7031.077 kb on + strand at 7031.078 kb on - strand at 7031.108 kb on + strand at 7031.108 kb on + strand at 7031.108 kb on + strand at 7031.109 kb on - strand at 7031.119 kb on + strand at 7031.119 kb on + strand at 7031.119 kb on + strand at 7031.191 kb on + strand at 7031.202 kb on + strand at 7031.203 kb on - strand at 7031.256 kb on + strand at 7031.256 kb on + strand at 7031.256 kb on + strand at 7031.256 kb on + strand at 7031.259 kb on - strand at 7031.275 kb on + strand at 7031.303 kb on - strand at 7031.384 kb on + strand at 7031.416 kb on - strand, within AO356_30125 at 7031.570 kb on - strand, within AO356_30125 at 7031.570 kb on - strand, within AO356_30125 at 7031.570 kb on - strand, within AO356_30125 at 7031.607 kb on - strand, within AO356_30125 at 7031.607 kb on - strand, within AO356_30125 at 7031.607 kb on - strand, within AO356_30125 at 7031.673 kb on + strand, within AO356_30125 at 7031.673 kb on + strand, within AO356_30125 at 7031.674 kb on - strand, within AO356_30125
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 7,029,206 + AO356_30115 0.68 +0.7 7,029,206 + AO356_30115 0.68 +0.4 7,029,206 + AO356_30115 0.68 +0.7 7,029,207 - AO356_30115 0.68 +0.1 7,029,207 - AO356_30115 0.68 -0.3 7,029,324 - AO356_30115 0.72 +0.1 7,029,596 + AO356_30115 0.82 -0.9 7,029,596 + AO356_30115 0.82 +0.6 7,030,086 + -0.7 7,030,124 + +0.1 7,030,124 + +0.1 7,030,124 + -0.2 7,030,253 + AO356_30120 0.21 -0.4 7,030,275 + AO356_30120 0.25 -1.1 7,030,349 + AO356_30120 0.37 +0.0 7,030,433 + AO356_30120 0.52 -0.7 7,030,433 + AO356_30120 0.52 -0.4 7,030,434 - AO356_30120 0.52 -0.7 7,030,434 - AO356_30120 0.52 +0.4 7,030,732 - -0.8 7,030,732 - +0.5 7,030,779 + +0.1 7,030,780 - -0.2 7,030,780 - +0.8 7,030,780 - -0.8 7,030,780 - +0.1 7,030,880 + -0.1 7,031,012 - +0.2 7,031,077 + +0.1 7,031,078 - +0.6 7,031,108 + -0.6 7,031,108 + +1.6 7,031,108 + +0.1 7,031,109 - -0.6 7,031,119 + -0.6 7,031,119 + +0.7 7,031,119 + +0.1 7,031,191 + -0.9 7,031,202 + -1.0 7,031,203 - +0.4 7,031,256 + +1.0 7,031,256 + +0.7 7,031,256 + +0.2 7,031,256 + +0.6 7,031,259 - +0.3 7,031,275 + +0.4 7,031,303 - +0.4 7,031,384 + -0.1 7,031,416 - AO356_30125 0.13 -0.1 7,031,570 - AO356_30125 0.32 -0.2 7,031,570 - AO356_30125 0.32 -0.4 7,031,570 - AO356_30125 0.32 -0.9 7,031,607 - AO356_30125 0.36 -0.5 7,031,607 - AO356_30125 0.36 +0.9 7,031,607 - AO356_30125 0.36 -0.0 7,031,673 + AO356_30125 0.44 +0.4 7,031,673 + AO356_30125 0.44 +0.4 7,031,674 - AO356_30125 0.44 -0.8
Or see this region's nucleotide sequence