Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_30105 and AO356_30110 are separated by 13 nucleotides AO356_30110 and AO356_30115 overlap by 4 nucleotides AO356_30115 and AO356_30120 are separated by 3 nucleotides
AO356_30105: AO356_30105 - sarcosine oxidase subunit beta, at 7,025,675 to 7,026,916
_30105
AO356_30110: AO356_30110 - sarcosine oxidase subunit delta, at 7,026,930 to 7,027,232
_30110
AO356_30115: AO356_30115 - aminomethyltransferase, at 7,027,229 to 7,030,126
_30115
AO356_30120: AO356_30120 - sarcosine oxidase, at 7,030,130 to 7,030,714
_30120
Position (kb) 7027
7028
7029
7030
7031 Strain fitness (log2 ratio) -1
0
1 at 7026.509 kb on + strand, within AO356_30105 at 7026.510 kb on - strand, within AO356_30105 at 7026.757 kb on + strand, within AO356_30105 at 7026.985 kb on - strand, within AO356_30110 at 7026.985 kb on - strand, within AO356_30110 at 7026.985 kb on - strand, within AO356_30110 at 7027.048 kb on - strand, within AO356_30110 at 7027.051 kb on + strand, within AO356_30110 at 7027.052 kb on - strand, within AO356_30110 at 7027.052 kb on - strand, within AO356_30110 at 7027.052 kb on - strand, within AO356_30110 at 7027.052 kb on - strand, within AO356_30110 at 7027.114 kb on - strand, within AO356_30110 at 7027.203 kb on - strand at 7027.385 kb on + strand at 7027.386 kb on - strand at 7027.386 kb on - strand at 7027.386 kb on - strand at 7027.589 kb on + strand, within AO356_30115 at 7027.589 kb on + strand, within AO356_30115 at 7027.590 kb on - strand, within AO356_30115 at 7027.592 kb on + strand, within AO356_30115 at 7027.604 kb on + strand, within AO356_30115 at 7027.604 kb on + strand, within AO356_30115 at 7027.605 kb on - strand, within AO356_30115 at 7027.709 kb on + strand, within AO356_30115 at 7027.709 kb on + strand, within AO356_30115 at 7027.838 kb on + strand, within AO356_30115 at 7027.838 kb on + strand, within AO356_30115 at 7027.839 kb on - strand, within AO356_30115 at 7027.967 kb on + strand, within AO356_30115 at 7027.967 kb on + strand, within AO356_30115 at 7027.967 kb on + strand, within AO356_30115 at 7027.997 kb on + strand, within AO356_30115 at 7027.997 kb on + strand, within AO356_30115 at 7027.997 kb on + strand, within AO356_30115 at 7027.997 kb on + strand, within AO356_30115 at 7027.998 kb on - strand, within AO356_30115 at 7027.998 kb on - strand, within AO356_30115 at 7027.998 kb on - strand, within AO356_30115 at 7027.998 kb on - strand, within AO356_30115 at 7028.120 kb on + strand, within AO356_30115 at 7028.121 kb on - strand, within AO356_30115 at 7028.175 kb on - strand, within AO356_30115 at 7028.288 kb on + strand, within AO356_30115 at 7028.288 kb on + strand, within AO356_30115 at 7028.312 kb on + strand, within AO356_30115 at 7028.312 kb on + strand, within AO356_30115 at 7028.344 kb on + strand, within AO356_30115 at 7028.344 kb on + strand, within AO356_30115 at 7028.345 kb on - strand, within AO356_30115 at 7028.421 kb on + strand, within AO356_30115 at 7028.421 kb on + strand, within AO356_30115 at 7028.442 kb on - strand, within AO356_30115 at 7028.686 kb on + strand, within AO356_30115 at 7028.686 kb on + strand, within AO356_30115 at 7028.686 kb on + strand, within AO356_30115 at 7028.686 kb on + strand, within AO356_30115 at 7028.687 kb on - strand, within AO356_30115 at 7029.062 kb on + strand, within AO356_30115 at 7029.066 kb on - strand, within AO356_30115 at 7029.206 kb on + strand, within AO356_30115 at 7029.206 kb on + strand, within AO356_30115 at 7029.206 kb on + strand, within AO356_30115 at 7029.207 kb on - strand, within AO356_30115 at 7029.207 kb on - strand, within AO356_30115 at 7029.324 kb on - strand, within AO356_30115 at 7029.596 kb on + strand, within AO356_30115 at 7029.596 kb on + strand, within AO356_30115 at 7030.086 kb on + strand at 7030.124 kb on + strand at 7030.124 kb on + strand at 7030.124 kb on + strand at 7030.253 kb on + strand, within AO356_30120 at 7030.275 kb on + strand, within AO356_30120 at 7030.349 kb on + strand, within AO356_30120 at 7030.433 kb on + strand, within AO356_30120 at 7030.433 kb on + strand, within AO356_30120 at 7030.434 kb on - strand, within AO356_30120 at 7030.434 kb on - strand, within AO356_30120 at 7030.732 kb on - strand at 7030.732 kb on - strand at 7030.779 kb on + strand at 7030.780 kb on - strand at 7030.780 kb on - strand at 7030.780 kb on - strand at 7030.780 kb on - strand at 7030.880 kb on + strand at 7031.012 kb on - strand at 7031.077 kb on + strand at 7031.078 kb on - strand at 7031.108 kb on + strand at 7031.108 kb on + strand at 7031.108 kb on + strand at 7031.109 kb on - strand at 7031.119 kb on + strand at 7031.119 kb on + strand at 7031.119 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 7,026,509 + AO356_30105 0.67 -0.8 7,026,510 - AO356_30105 0.67 -0.8 7,026,757 + AO356_30105 0.87 -0.4 7,026,985 - AO356_30110 0.18 -0.4 7,026,985 - AO356_30110 0.18 +0.9 7,026,985 - AO356_30110 0.18 +0.3 7,027,048 - AO356_30110 0.39 -0.2 7,027,051 + AO356_30110 0.40 +0.6 7,027,052 - AO356_30110 0.40 +0.6 7,027,052 - AO356_30110 0.40 -0.5 7,027,052 - AO356_30110 0.40 +0.1 7,027,052 - AO356_30110 0.40 +0.1 7,027,114 - AO356_30110 0.61 +0.3 7,027,203 - -0.4 7,027,385 + -0.8 7,027,386 - -0.6 7,027,386 - -0.5 7,027,386 - -0.0 7,027,589 + AO356_30115 0.12 -0.0 7,027,589 + AO356_30115 0.12 +0.2 7,027,590 - AO356_30115 0.12 -0.7 7,027,592 + AO356_30115 0.13 +1.0 7,027,604 + AO356_30115 0.13 +0.0 7,027,604 + AO356_30115 0.13 -0.6 7,027,605 - AO356_30115 0.13 +0.5 7,027,709 + AO356_30115 0.17 -0.2 7,027,709 + AO356_30115 0.17 +0.1 7,027,838 + AO356_30115 0.21 -0.0 7,027,838 + AO356_30115 0.21 +0.1 7,027,839 - AO356_30115 0.21 +0.3 7,027,967 + AO356_30115 0.25 -0.0 7,027,967 + AO356_30115 0.25 +0.4 7,027,967 + AO356_30115 0.25 -0.8 7,027,997 + AO356_30115 0.27 -1.2 7,027,997 + AO356_30115 0.27 +1.1 7,027,997 + AO356_30115 0.27 +0.2 7,027,997 + AO356_30115 0.27 -0.2 7,027,998 - AO356_30115 0.27 +0.5 7,027,998 - AO356_30115 0.27 -1.2 7,027,998 - AO356_30115 0.27 -1.6 7,027,998 - AO356_30115 0.27 -0.8 7,028,120 + AO356_30115 0.31 +0.2 7,028,121 - AO356_30115 0.31 +0.3 7,028,175 - AO356_30115 0.33 -1.0 7,028,288 + AO356_30115 0.37 +0.0 7,028,288 + AO356_30115 0.37 +0.2 7,028,312 + AO356_30115 0.37 +0.2 7,028,312 + AO356_30115 0.37 -0.2 7,028,344 + AO356_30115 0.38 -0.2 7,028,344 + AO356_30115 0.38 +0.7 7,028,345 - AO356_30115 0.39 +0.4 7,028,421 + AO356_30115 0.41 +0.8 7,028,421 + AO356_30115 0.41 +0.4 7,028,442 - AO356_30115 0.42 -0.1 7,028,686 + AO356_30115 0.50 +0.5 7,028,686 + AO356_30115 0.50 +0.4 7,028,686 + AO356_30115 0.50 +0.3 7,028,686 + AO356_30115 0.50 +1.1 7,028,687 - AO356_30115 0.50 +0.2 7,029,062 + AO356_30115 0.63 -0.2 7,029,066 - AO356_30115 0.63 -0.5 7,029,206 + AO356_30115 0.68 +0.7 7,029,206 + AO356_30115 0.68 +0.4 7,029,206 + AO356_30115 0.68 +0.7 7,029,207 - AO356_30115 0.68 +0.1 7,029,207 - AO356_30115 0.68 -0.3 7,029,324 - AO356_30115 0.72 +0.1 7,029,596 + AO356_30115 0.82 -0.9 7,029,596 + AO356_30115 0.82 +0.6 7,030,086 + -0.7 7,030,124 + +0.1 7,030,124 + +0.1 7,030,124 + -0.2 7,030,253 + AO356_30120 0.21 -0.4 7,030,275 + AO356_30120 0.25 -1.1 7,030,349 + AO356_30120 0.37 +0.0 7,030,433 + AO356_30120 0.52 -0.7 7,030,433 + AO356_30120 0.52 -0.4 7,030,434 - AO356_30120 0.52 -0.7 7,030,434 - AO356_30120 0.52 +0.4 7,030,732 - -0.8 7,030,732 - +0.5 7,030,779 + +0.1 7,030,780 - -0.2 7,030,780 - +0.8 7,030,780 - -0.8 7,030,780 - +0.1 7,030,880 + -0.1 7,031,012 - +0.2 7,031,077 + +0.1 7,031,078 - +0.6 7,031,108 + -0.6 7,031,108 + +1.6 7,031,108 + +0.1 7,031,109 - -0.6 7,031,119 + -0.6 7,031,119 + +0.7 7,031,119 + +0.1
Or see this region's nucleotide sequence