Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25045 and AO356_25050 overlap by 8 nucleotides AO356_25050 and AO356_25055 overlap by 4 nucleotides AO356_25055 and AO356_25060 are separated by 2 nucleotides AO356_25060 and AO356_25065 overlap by 4 nucleotides AO356_25065 and AO356_25070 are separated by 179 nucleotides AO356_25070 and AO356_25075 are separated by 24 nucleotides
AO356_25045: AO356_25045 - protein nirG, at 5,674,392 to 5,674,835
_25045
AO356_25050: AO356_25050 - protein nirL, at 5,674,828 to 5,675,340
_25050
AO356_25055: AO356_25055 - AsnC family transcriptional regulator, at 5,675,337 to 5,675,795
_25055
AO356_25060: AO356_25060 - protein nirF, at 5,675,798 to 5,676,976
_25060
AO356_25065: AO356_25065 - disulfide bond formation protein DsbD, at 5,676,973 to 5,677,302
_25065
AO356_25070: AO356_25070 - cytochrome C biogenesis protein CcsA, at 5,677,482 to 5,677,790
_25070
AO356_25075: AO356_25075 - nitrite reductase, at 5,677,815 to 5,679,494
_25075
Position (kb)
5675
5676
5677 Strain fitness (log2 ratio)
-2
-1
0
1 at 5674.903 kb on + strand, within AO356_25050 at 5674.904 kb on - strand, within AO356_25050 at 5674.904 kb on - strand, within AO356_25050 at 5675.006 kb on + strand, within AO356_25050 at 5675.006 kb on + strand, within AO356_25050 at 5675.006 kb on + strand, within AO356_25050 at 5675.006 kb on + strand, within AO356_25050 at 5675.007 kb on - strand, within AO356_25050 at 5675.007 kb on - strand, within AO356_25050 at 5675.007 kb on - strand, within AO356_25050 at 5675.491 kb on + strand, within AO356_25055 at 5675.491 kb on + strand, within AO356_25055 at 5675.491 kb on + strand, within AO356_25055 at 5675.509 kb on + strand, within AO356_25055 at 5675.509 kb on + strand, within AO356_25055 at 5675.510 kb on - strand, within AO356_25055 at 5675.641 kb on - strand, within AO356_25055 at 5675.723 kb on - strand, within AO356_25055 at 5676.234 kb on + strand, within AO356_25060 at 5676.234 kb on + strand, within AO356_25060 at 5676.351 kb on + strand, within AO356_25060 at 5676.351 kb on + strand, within AO356_25060 at 5676.351 kb on + strand, within AO356_25060 at 5676.351 kb on + strand, within AO356_25060 at 5676.351 kb on + strand, within AO356_25060 at 5676.351 kb on + strand, within AO356_25060 at 5676.352 kb on - strand, within AO356_25060 at 5676.352 kb on - strand, within AO356_25060 at 5676.352 kb on - strand, within AO356_25060 at 5676.352 kb on - strand, within AO356_25060 at 5676.352 kb on - strand, within AO356_25060 at 5677.161 kb on - strand, within AO356_25065 at 5677.323 kb on + strand at 5677.344 kb on + strand at 5677.345 kb on - strand at 5677.345 kb on - strand at 5677.442 kb on + strand at 5677.442 kb on + strand at 5677.442 kb on + strand at 5677.442 kb on + strand at 5677.442 kb on + strand at 5677.443 kb on - strand at 5677.443 kb on - strand at 5677.446 kb on + strand at 5677.446 kb on + strand at 5677.446 kb on + strand at 5677.446 kb on + strand at 5677.446 kb on + strand at 5677.447 kb on - strand at 5677.447 kb on - strand at 5677.447 kb on - strand at 5677.447 kb on - strand at 5677.487 kb on + strand at 5677.781 kb on + strand at 5677.781 kb on + strand at 5677.803 kb on + strand at 5677.819 kb on + strand at 5677.819 kb on + strand at 5677.819 kb on + strand at 5677.819 kb on + strand at 5677.820 kb on - strand at 5677.820 kb on - strand at 5677.820 kb on - strand at 5677.820 kb on - strand at 5677.840 kb on + strand at 5677.841 kb on - strand at 5677.841 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 5,674,903 + AO356_25050 0.15 -0.1 5,674,904 - AO356_25050 0.15 -0.0 5,674,904 - AO356_25050 0.15 +0.5 5,675,006 + AO356_25050 0.35 -0.2 5,675,006 + AO356_25050 0.35 -0.1 5,675,006 + AO356_25050 0.35 -0.1 5,675,006 + AO356_25050 0.35 -0.3 5,675,007 - AO356_25050 0.35 +0.4 5,675,007 - AO356_25050 0.35 +0.0 5,675,007 - AO356_25050 0.35 +0.6 5,675,491 + AO356_25055 0.34 +0.6 5,675,491 + AO356_25055 0.34 +0.7 5,675,491 + AO356_25055 0.34 -0.1 5,675,509 + AO356_25055 0.37 -1.5 5,675,509 + AO356_25055 0.37 -0.6 5,675,510 - AO356_25055 0.38 -1.5 5,675,641 - AO356_25055 0.66 +0.4 5,675,723 - AO356_25055 0.84 +0.5 5,676,234 + AO356_25060 0.37 -0.8 5,676,234 + AO356_25060 0.37 +0.2 5,676,351 + AO356_25060 0.47 +0.1 5,676,351 + AO356_25060 0.47 +0.8 5,676,351 + AO356_25060 0.47 -0.5 5,676,351 + AO356_25060 0.47 +0.1 5,676,351 + AO356_25060 0.47 -1.2 5,676,351 + AO356_25060 0.47 -0.9 5,676,352 - AO356_25060 0.47 +0.2 5,676,352 - AO356_25060 0.47 -0.4 5,676,352 - AO356_25060 0.47 -0.4 5,676,352 - AO356_25060 0.47 +0.5 5,676,352 - AO356_25060 0.47 -1.7 5,677,161 - AO356_25065 0.57 +0.1 5,677,323 + -0.2 5,677,344 + -0.1 5,677,345 - -1.1 5,677,345 - +0.2 5,677,442 + +0.1 5,677,442 + -0.0 5,677,442 + -0.1 5,677,442 + +0.1 5,677,442 + -0.7 5,677,443 - +0.6 5,677,443 - -0.9 5,677,446 + -0.9 5,677,446 + -0.5 5,677,446 + +0.2 5,677,446 + -0.2 5,677,446 + +0.4 5,677,447 - +1.1 5,677,447 - +0.9 5,677,447 - -1.0 5,677,447 - -2.1 5,677,487 + +0.8 5,677,781 + +1.1 5,677,781 + +0.5 5,677,803 + +0.0 5,677,819 + -0.4 5,677,819 + +0.0 5,677,819 + +0.1 5,677,819 + +0.4 5,677,820 - -0.3 5,677,820 - +0.4 5,677,820 - -0.3 5,677,820 - -0.4 5,677,840 + +0.6 5,677,841 - +0.1 5,677,841 - -0.5
Or see this region's nucleotide sequence