Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22905 and AO356_22910 are separated by 4 nucleotides AO356_22910 and AO356_22915 are separated by 56 nucleotides AO356_22915 and AO356_22920 are separated by 41 nucleotides
AO356_22905: AO356_22905 - transcriptional regulator, at 5,115,225 to 5,115,926
_22905
AO356_22910: AO356_22910 - histidine kinase, at 5,115,931 to 5,117,232
_22910
AO356_22915: AO356_22915 - hypothetical protein, at 5,117,289 to 5,117,930
_22915
AO356_22920: AO356_22920 - nicotinamidase, at 5,117,972 to 5,118,625
_22920
Position (kb) 5115
5116
5117
5118 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5115.023 kb on + strand at 5115.024 kb on - strand at 5115.037 kb on + strand at 5115.053 kb on - strand at 5115.053 kb on - strand at 5115.068 kb on + strand at 5115.068 kb on + strand at 5115.069 kb on - strand at 5115.069 kb on - strand at 5115.069 kb on - strand at 5115.069 kb on - strand at 5115.069 kb on - strand at 5115.070 kb on + strand at 5115.071 kb on - strand at 5115.282 kb on + strand at 5115.519 kb on + strand, within AO356_22905 at 5115.519 kb on + strand, within AO356_22905 at 5115.520 kb on - strand, within AO356_22905 at 5115.520 kb on - strand, within AO356_22905 at 5115.520 kb on - strand, within AO356_22905 at 5115.520 kb on - strand, within AO356_22905 at 5115.520 kb on - strand, within AO356_22905 at 5115.702 kb on + strand, within AO356_22905 at 5115.702 kb on + strand, within AO356_22905 at 5115.703 kb on - strand, within AO356_22905 at 5115.703 kb on - strand, within AO356_22905 at 5115.955 kb on + strand at 5115.992 kb on - strand at 5115.992 kb on - strand at 5115.992 kb on - strand at 5116.043 kb on - strand at 5116.043 kb on - strand at 5116.055 kb on - strand at 5116.345 kb on + strand, within AO356_22910 at 5116.346 kb on - strand, within AO356_22910 at 5116.346 kb on - strand, within AO356_22910 at 5116.479 kb on + strand, within AO356_22910 at 5116.479 kb on + strand, within AO356_22910 at 5116.741 kb on + strand, within AO356_22910 at 5116.741 kb on + strand, within AO356_22910 at 5116.742 kb on - strand, within AO356_22910 at 5117.312 kb on + strand at 5117.373 kb on + strand, within AO356_22915 at 5117.450 kb on - strand, within AO356_22915 at 5117.464 kb on + strand, within AO356_22915 at 5117.464 kb on + strand, within AO356_22915 at 5117.465 kb on - strand, within AO356_22915 at 5117.465 kb on - strand, within AO356_22915 at 5117.465 kb on - strand, within AO356_22915 at 5117.524 kb on + strand, within AO356_22915 at 5117.524 kb on + strand, within AO356_22915 at 5117.524 kb on + strand, within AO356_22915 at 5117.524 kb on + strand, within AO356_22915 at 5117.525 kb on - strand, within AO356_22915 at 5117.527 kb on + strand, within AO356_22915 at 5117.527 kb on + strand, within AO356_22915 at 5117.527 kb on + strand, within AO356_22915 at 5117.527 kb on + strand, within AO356_22915 at 5117.527 kb on + strand, within AO356_22915 at 5117.528 kb on - strand, within AO356_22915 at 5117.605 kb on + strand, within AO356_22915 at 5117.605 kb on + strand, within AO356_22915 at 5117.605 kb on + strand, within AO356_22915 at 5117.606 kb on - strand, within AO356_22915 at 5117.865 kb on - strand, within AO356_22915 at 5117.960 kb on + strand at 5117.960 kb on + strand at 5117.960 kb on + strand at 5117.960 kb on + strand at 5117.960 kb on + strand at 5117.960 kb on + strand at 5117.961 kb on - strand at 5117.961 kb on - strand at 5117.961 kb on - strand at 5117.961 kb on - strand at 5117.961 kb on - strand at 5117.963 kb on + strand at 5117.964 kb on - strand at 5118.174 kb on + strand, within AO356_22920
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 5,115,023 + +0.4 5,115,024 - -0.2 5,115,037 + +0.2 5,115,053 - -1.1 5,115,053 - -1.2 5,115,068 + +0.5 5,115,068 + +0.6 5,115,069 - -0.9 5,115,069 - -0.1 5,115,069 - -0.4 5,115,069 - -0.0 5,115,069 - -0.3 5,115,070 + +0.1 5,115,071 - -1.6 5,115,282 + -2.2 5,115,519 + AO356_22905 0.42 -2.8 5,115,519 + AO356_22905 0.42 -0.2 5,115,520 - AO356_22905 0.42 -1.5 5,115,520 - AO356_22905 0.42 +1.3 5,115,520 - AO356_22905 0.42 -1.1 5,115,520 - AO356_22905 0.42 -3.1 5,115,520 - AO356_22905 0.42 -0.9 5,115,702 + AO356_22905 0.68 -1.5 5,115,702 + AO356_22905 0.68 -1.1 5,115,703 - AO356_22905 0.68 -1.8 5,115,703 - AO356_22905 0.68 -1.1 5,115,955 + -0.1 5,115,992 - -1.4 5,115,992 - -0.8 5,115,992 - -0.9 5,116,043 - -0.7 5,116,043 - -1.7 5,116,055 - -1.4 5,116,345 + AO356_22910 0.32 -1.9 5,116,346 - AO356_22910 0.32 +0.1 5,116,346 - AO356_22910 0.32 -0.9 5,116,479 + AO356_22910 0.42 +0.6 5,116,479 + AO356_22910 0.42 +0.2 5,116,741 + AO356_22910 0.62 +3.0 5,116,741 + AO356_22910 0.62 +1.3 5,116,742 - AO356_22910 0.62 +0.6 5,117,312 + -0.1 5,117,373 + AO356_22915 0.13 +0.6 5,117,450 - AO356_22915 0.25 +0.4 5,117,464 + AO356_22915 0.27 -0.2 5,117,464 + AO356_22915 0.27 -0.8 5,117,465 - AO356_22915 0.27 -0.5 5,117,465 - AO356_22915 0.27 +0.0 5,117,465 - AO356_22915 0.27 +0.2 5,117,524 + AO356_22915 0.37 -1.4 5,117,524 + AO356_22915 0.37 -0.2 5,117,524 + AO356_22915 0.37 +0.1 5,117,524 + AO356_22915 0.37 +0.3 5,117,525 - AO356_22915 0.37 -0.1 5,117,527 + AO356_22915 0.37 -0.6 5,117,527 + AO356_22915 0.37 +0.3 5,117,527 + AO356_22915 0.37 +0.7 5,117,527 + AO356_22915 0.37 +0.1 5,117,527 + AO356_22915 0.37 +0.0 5,117,528 - AO356_22915 0.37 -0.2 5,117,605 + AO356_22915 0.49 +0.5 5,117,605 + AO356_22915 0.49 -0.5 5,117,605 + AO356_22915 0.49 -1.2 5,117,606 - AO356_22915 0.49 +0.4 5,117,865 - AO356_22915 0.90 +0.4 5,117,960 + +0.1 5,117,960 + +0.9 5,117,960 + -0.3 5,117,960 + +0.4 5,117,960 + -0.4 5,117,960 + -0.0 5,117,961 - +0.6 5,117,961 - -1.2 5,117,961 - -0.3 5,117,961 - +0.7 5,117,961 - +0.4 5,117,963 + -0.2 5,117,964 - +0.1 5,118,174 + AO356_22920 0.31 -0.1
Or see this region's nucleotide sequence