Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_15910 and AO356_15915 overlap by 11 nucleotides AO356_15915 and AO356_15920 are separated by 125 nucleotides AO356_15920 and AO356_15925 are separated by 150 nucleotides
AO356_15910: AO356_15910 - histidine kinase, at 3,602,458 to 3,604,047
_15910
AO356_15915: AO356_15915 - hypothetical protein, at 3,604,037 to 3,604,270
_15915
AO356_15920: AO356_15920 - DNA transporter, at 3,604,396 to 3,605,412
_15920
AO356_15925: AO356_15925 - ribosomal large subunit pseudouridine synthase D, at 3,605,563 to 3,606,525
_15925
Position (kb)
3604
3605
3606 Strain fitness (log2 ratio)
-2
-1
0
1 at 3603.591 kb on - strand, within AO356_15910 at 3603.591 kb on - strand, within AO356_15910 at 3603.591 kb on - strand, within AO356_15910 at 3603.591 kb on - strand, within AO356_15910 at 3603.591 kb on - strand, within AO356_15910 at 3603.872 kb on + strand, within AO356_15910 at 3603.872 kb on + strand, within AO356_15910 at 3603.872 kb on + strand, within AO356_15910 at 3603.872 kb on + strand, within AO356_15910 at 3603.872 kb on + strand, within AO356_15910 at 3603.873 kb on - strand, within AO356_15910 at 3603.873 kb on - strand, within AO356_15910 at 3603.969 kb on + strand at 3603.980 kb on + strand at 3603.990 kb on - strand at 3603.990 kb on - strand at 3604.086 kb on + strand, within AO356_15915 at 3604.086 kb on + strand, within AO356_15915 at 3604.087 kb on - strand, within AO356_15915 at 3604.087 kb on - strand, within AO356_15915 at 3604.087 kb on - strand, within AO356_15915 at 3604.087 kb on - strand, within AO356_15915 at 3604.130 kb on + strand, within AO356_15915 at 3604.130 kb on + strand, within AO356_15915 at 3604.131 kb on - strand, within AO356_15915 at 3604.131 kb on - strand, within AO356_15915 at 3604.226 kb on + strand, within AO356_15915 at 3604.226 kb on + strand, within AO356_15915 at 3604.227 kb on - strand, within AO356_15915 at 3604.259 kb on + strand at 3604.259 kb on + strand at 3604.260 kb on - strand at 3604.273 kb on + strand at 3604.305 kb on + strand at 3604.306 kb on - strand at 3604.336 kb on + strand at 3604.336 kb on + strand at 3604.337 kb on - strand at 3604.337 kb on - strand at 3604.337 kb on - strand at 3604.380 kb on + strand at 3604.380 kb on + strand at 3604.381 kb on - strand at 3604.381 kb on - strand at 3604.421 kb on + strand at 3604.421 kb on + strand at 3604.421 kb on + strand at 3604.421 kb on + strand at 3604.422 kb on - strand at 3604.422 kb on - strand at 3604.422 kb on - strand at 3604.546 kb on + strand, within AO356_15920 at 3604.546 kb on + strand, within AO356_15920 at 3604.547 kb on - strand, within AO356_15920 at 3604.547 kb on - strand, within AO356_15920 at 3604.547 kb on - strand, within AO356_15920 at 3604.547 kb on - strand, within AO356_15920 at 3604.695 kb on - strand, within AO356_15920 at 3604.695 kb on - strand, within AO356_15920 at 3605.429 kb on - strand at 3605.464 kb on - strand at 3605.572 kb on - strand at 3605.577 kb on - strand at 3605.577 kb on - strand at 3605.577 kb on - strand at 3605.821 kb on + strand, within AO356_15925 at 3605.821 kb on + strand, within AO356_15925 at 3606.070 kb on + strand, within AO356_15925 at 3606.070 kb on + strand, within AO356_15925 at 3606.070 kb on + strand, within AO356_15925 at 3606.070 kb on + strand, within AO356_15925 at 3606.070 kb on + strand, within AO356_15925 at 3606.070 kb on + strand, within AO356_15925 at 3606.070 kb on + strand, within AO356_15925 at 3606.071 kb on - strand, within AO356_15925 at 3606.071 kb on - strand, within AO356_15925 at 3606.071 kb on - strand, within AO356_15925 at 3606.071 kb on - strand, within AO356_15925 at 3606.071 kb on - strand, within AO356_15925 at 3606.071 kb on - strand, within AO356_15925 at 3606.071 kb on - strand, within AO356_15925 at 3606.255 kb on + strand, within AO356_15925 at 3606.316 kb on + strand, within AO356_15925
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 3,603,591 - AO356_15910 0.71 +0.5 3,603,591 - AO356_15910 0.71 -0.4 3,603,591 - AO356_15910 0.71 +0.1 3,603,591 - AO356_15910 0.71 +0.9 3,603,591 - AO356_15910 0.71 +1.1 3,603,872 + AO356_15910 0.89 +0.4 3,603,872 + AO356_15910 0.89 +0.3 3,603,872 + AO356_15910 0.89 +0.3 3,603,872 + AO356_15910 0.89 +0.7 3,603,872 + AO356_15910 0.89 -0.8 3,603,873 - AO356_15910 0.89 -0.2 3,603,873 - AO356_15910 0.89 +0.0 3,603,969 + -1.5 3,603,980 + -0.1 3,603,990 - +0.3 3,603,990 - -0.2 3,604,086 + AO356_15915 0.21 +0.0 3,604,086 + AO356_15915 0.21 -2.2 3,604,087 - AO356_15915 0.21 -0.4 3,604,087 - AO356_15915 0.21 +0.2 3,604,087 - AO356_15915 0.21 -0.8 3,604,087 - AO356_15915 0.21 -0.1 3,604,130 + AO356_15915 0.40 -0.1 3,604,130 + AO356_15915 0.40 +0.6 3,604,131 - AO356_15915 0.40 +0.1 3,604,131 - AO356_15915 0.40 +0.1 3,604,226 + AO356_15915 0.81 +0.4 3,604,226 + AO356_15915 0.81 -0.2 3,604,227 - AO356_15915 0.81 -0.2 3,604,259 + +0.5 3,604,259 + +0.8 3,604,260 - +0.5 3,604,273 + +0.0 3,604,305 + -0.0 3,604,306 - -0.7 3,604,336 + +1.1 3,604,336 + +0.6 3,604,337 - +0.3 3,604,337 - +0.0 3,604,337 - +0.9 3,604,380 + +1.1 3,604,380 + -0.1 3,604,381 - +0.5 3,604,381 - -0.7 3,604,421 + -1.3 3,604,421 + -0.6 3,604,421 + -1.1 3,604,421 + -0.1 3,604,422 - +0.0 3,604,422 - -0.6 3,604,422 - +0.8 3,604,546 + AO356_15920 0.15 -1.0 3,604,546 + AO356_15920 0.15 -1.1 3,604,547 - AO356_15920 0.15 -1.0 3,604,547 - AO356_15920 0.15 -1.4 3,604,547 - AO356_15920 0.15 -0.6 3,604,547 - AO356_15920 0.15 -1.0 3,604,695 - AO356_15920 0.29 -1.3 3,604,695 - AO356_15920 0.29 +1.1 3,605,429 - +1.1 3,605,464 - +0.5 3,605,572 - -0.3 3,605,577 - -2.6 3,605,577 - +1.3 3,605,577 - +0.5 3,605,821 + AO356_15925 0.27 -0.1 3,605,821 + AO356_15925 0.27 -1.7 3,606,070 + AO356_15925 0.53 -0.3 3,606,070 + AO356_15925 0.53 +0.6 3,606,070 + AO356_15925 0.53 +0.6 3,606,070 + AO356_15925 0.53 +0.3 3,606,070 + AO356_15925 0.53 -0.5 3,606,070 + AO356_15925 0.53 -0.1 3,606,070 + AO356_15925 0.53 -0.8 3,606,071 - AO356_15925 0.53 +0.9 3,606,071 - AO356_15925 0.53 +0.7 3,606,071 - AO356_15925 0.53 -1.6 3,606,071 - AO356_15925 0.53 -0.0 3,606,071 - AO356_15925 0.53 -0.1 3,606,071 - AO356_15925 0.53 -0.6 3,606,071 - AO356_15925 0.53 +0.2 3,606,255 + AO356_15925 0.72 +0.2 3,606,316 + AO356_15925 0.78 -0.3
Or see this region's nucleotide sequence