Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_00970 and AO356_00975 are separated by 133 nucleotides AO356_00975 and AO356_00980 are separated by 204 nucleotides AO356_00980 and AO356_00985 are separated by 5 nucleotides AO356_00985 and AO356_00990 are separated by 156 nucleotides
AO356_00970: AO356_00970 - LacI family transcriptional regulator, at 220,733 to 221,692
_00970
AO356_00975: AO356_00975 - hypothetical protein, at 221,826 to 222,278
_00975
AO356_00980: AO356_00980 - DNA damage resistance protein <small>(from data)</small>, at 222,483 to 222,782
_00980
AO356_00985: AO356_00985 - deoxyribonuclease, at 222,788 to 223,477
_00985
AO356_00990: AO356_00990 - penicillin-binding protein activator LpoB, at 223,634 to 223,999
_00990
Position (kb) 222
223 Strain fitness (log2 ratio) -2
-1
0
1 at 221.517 kb on + strand, within AO356_00970 at 221.518 kb on - strand, within AO356_00970 at 221.518 kb on - strand, within AO356_00970 at 221.624 kb on - strand at 221.709 kb on + strand at 221.709 kb on + strand at 221.710 kb on - strand at 221.715 kb on + strand at 221.715 kb on + strand at 221.716 kb on - strand at 221.770 kb on + strand at 221.771 kb on - strand at 221.771 kb on - strand at 221.771 kb on - strand at 221.775 kb on + strand at 221.837 kb on + strand at 221.837 kb on + strand at 221.838 kb on - strand at 221.977 kb on + strand, within AO356_00975 at 222.161 kb on + strand, within AO356_00975 at 222.305 kb on + strand at 222.305 kb on + strand at 222.306 kb on - strand at 222.393 kb on + strand at 222.394 kb on - strand at 222.472 kb on + strand at 222.472 kb on + strand at 222.472 kb on + strand at 222.473 kb on - strand at 222.473 kb on - strand at 222.480 kb on + strand at 222.498 kb on + strand at 222.519 kb on - strand, within AO356_00980 at 222.528 kb on - strand, within AO356_00980 at 222.565 kb on + strand, within AO356_00980 at 222.566 kb on - strand, within AO356_00980 at 222.566 kb on - strand, within AO356_00980 at 222.566 kb on - strand, within AO356_00980 at 222.574 kb on - strand, within AO356_00980 at 222.574 kb on - strand, within AO356_00980 at 222.574 kb on - strand, within AO356_00980 at 222.575 kb on + strand, within AO356_00980 at 222.575 kb on + strand, within AO356_00980 at 222.576 kb on - strand, within AO356_00980 at 222.805 kb on - strand at 222.893 kb on + strand, within AO356_00985 at 222.893 kb on + strand, within AO356_00985 at 222.893 kb on + strand, within AO356_00985 at 222.893 kb on + strand, within AO356_00985 at 222.894 kb on - strand, within AO356_00985 at 222.894 kb on - strand, within AO356_00985 at 222.894 kb on - strand, within AO356_00985 at 222.894 kb on - strand, within AO356_00985 at 222.917 kb on + strand, within AO356_00985 at 222.918 kb on - strand, within AO356_00985 at 222.928 kb on + strand, within AO356_00985 at 222.928 kb on + strand, within AO356_00985 at 222.929 kb on - strand, within AO356_00985 at 222.932 kb on + strand, within AO356_00985 at 222.933 kb on - strand, within AO356_00985 at 222.972 kb on + strand, within AO356_00985 at 222.972 kb on + strand, within AO356_00985 at 223.018 kb on + strand, within AO356_00985 at 223.018 kb on + strand, within AO356_00985 at 223.018 kb on + strand, within AO356_00985 at 223.018 kb on + strand, within AO356_00985 at 223.018 kb on + strand, within AO356_00985 at 223.019 kb on - strand, within AO356_00985 at 223.019 kb on - strand, within AO356_00985 at 223.019 kb on - strand, within AO356_00985 at 223.103 kb on + strand, within AO356_00985 at 223.103 kb on + strand, within AO356_00985 at 223.104 kb on - strand, within AO356_00985 at 223.104 kb on - strand, within AO356_00985 at 223.295 kb on + strand, within AO356_00985 at 223.295 kb on + strand, within AO356_00985 at 223.295 kb on + strand, within AO356_00985 at 223.301 kb on + strand, within AO356_00985 at 223.302 kb on - strand, within AO356_00985 at 223.317 kb on - strand, within AO356_00985 at 223.352 kb on + strand, within AO356_00985 at 223.352 kb on + strand, within AO356_00985 at 223.352 kb on + strand, within AO356_00985 at 223.352 kb on + strand, within AO356_00985 at 223.352 kb on + strand, within AO356_00985 at 223.373 kb on + strand, within AO356_00985 at 223.635 kb on + strand at 223.636 kb on - strand at 223.651 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 221,517 + AO356_00970 0.82 -0.3 221,518 - AO356_00970 0.82 +0.2 221,518 - AO356_00970 0.82 -0.6 221,624 - -0.2 221,709 + -0.3 221,709 + -0.4 221,710 - +0.0 221,715 + -0.2 221,715 + +0.6 221,716 - +0.3 221,770 + -0.1 221,771 - -0.4 221,771 - +1.0 221,771 - -0.9 221,775 + -1.3 221,837 + +0.3 221,837 + +0.6 221,838 - -0.3 221,977 + AO356_00975 0.33 +0.0 222,161 + AO356_00975 0.74 -2.5 222,305 + +0.1 222,305 + +0.2 222,306 - -2.1 222,393 + -0.1 222,394 - +0.6 222,472 + -0.9 222,472 + -0.8 222,472 + +0.2 222,473 - +0.2 222,473 - -0.6 222,480 + -1.6 222,498 + -0.6 222,519 - AO356_00980 0.12 -0.5 222,528 - AO356_00980 0.15 +0.9 222,565 + AO356_00980 0.27 -0.3 222,566 - AO356_00980 0.28 -0.3 222,566 - AO356_00980 0.28 -1.3 222,566 - AO356_00980 0.28 -1.1 222,574 - AO356_00980 0.30 +0.9 222,574 - AO356_00980 0.30 -0.1 222,574 - AO356_00980 0.30 +0.1 222,575 + AO356_00980 0.31 -0.4 222,575 + AO356_00980 0.31 -1.4 222,576 - AO356_00980 0.31 +0.0 222,805 - +0.8 222,893 + AO356_00985 0.15 +0.2 222,893 + AO356_00985 0.15 +0.1 222,893 + AO356_00985 0.15 +0.4 222,893 + AO356_00985 0.15 -0.9 222,894 - AO356_00985 0.15 -0.8 222,894 - AO356_00985 0.15 -1.2 222,894 - AO356_00985 0.15 +0.6 222,894 - AO356_00985 0.15 +1.7 222,917 + AO356_00985 0.19 -0.4 222,918 - AO356_00985 0.19 +0.0 222,928 + AO356_00985 0.20 -0.4 222,928 + AO356_00985 0.20 -0.5 222,929 - AO356_00985 0.20 +0.4 222,932 + AO356_00985 0.21 +0.4 222,933 - AO356_00985 0.21 -0.7 222,972 + AO356_00985 0.27 -0.2 222,972 + AO356_00985 0.27 +0.5 223,018 + AO356_00985 0.33 +0.5 223,018 + AO356_00985 0.33 -0.1 223,018 + AO356_00985 0.33 -1.3 223,018 + AO356_00985 0.33 -0.4 223,018 + AO356_00985 0.33 -0.3 223,019 - AO356_00985 0.33 -1.6 223,019 - AO356_00985 0.33 -0.0 223,019 - AO356_00985 0.33 -0.5 223,103 + AO356_00985 0.46 +0.3 223,103 + AO356_00985 0.46 -0.5 223,104 - AO356_00985 0.46 -1.3 223,104 - AO356_00985 0.46 -1.3 223,295 + AO356_00985 0.73 -0.3 223,295 + AO356_00985 0.73 -0.2 223,295 + AO356_00985 0.73 +0.7 223,301 + AO356_00985 0.74 +0.3 223,302 - AO356_00985 0.74 -0.5 223,317 - AO356_00985 0.77 -1.4 223,352 + AO356_00985 0.82 -0.5 223,352 + AO356_00985 0.82 -0.2 223,352 + AO356_00985 0.82 -0.0 223,352 + AO356_00985 0.82 -0.2 223,352 + AO356_00985 0.82 -0.7 223,373 + AO356_00985 0.85 +0.3 223,635 + -0.0 223,636 - +0.4 223,651 - +0.7
Or see this region's nucleotide sequence